Can Gong, Xiang-Ting Lu, Shi-Ding Zhang, Kun Xiao, Xu Xu
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引用次数: 0
摘要
采用多孔石墨碳柱和正辛烷-异丙醇流动相进行液相色谱-质谱法分离各种食用油中的三酰甘油(TAGs),旨在鉴别大豆油、玉米油和葵花籽油中的猪油掺假。实验采用 Hypercarb 色谱柱(2.1 mm × 100 mm,5 µm)和正辛烷-异丙醇(70:30,V/V)流动相,流速为 0.25 mL-min-1,柱温为 60 °C。检测采用常压化学电离质谱法。对各种食用油样品的分析表明,同一类型的油具有相似的 TAG 组成,而不同类型的油则表现出不同的 TAG 特征。在不同的食用油中观察到甘油三酯组成的明显变化。基于液相色谱-质谱分析,通过主成分分析和正交偏最小二乘法判别分析,确定了猪油中的特征成分 1-硬脂酸-2-棕榈酸-3-油酸甘油酯(SPO),其中可能还包括 PSO。该成分是检测大豆油、玉米油和葵花籽油中低至 0.1% 的猪油掺假的标记。该技术为识别这些食用油中的猪油掺假提供了一种精确有效的方法。
Detection of lard adulteration in 3 kinds of vegetable oils by liquid chromatography-mass spectrometry with porous graphite carbon column.
Liquid chromatography‒mass spectrometry employing porous graphite carbon columns and an n-octane-isopropanol mobile phase was utilized for the separation of triacylglycerols (TAGs) in various edible oils, aiming to identify lard adulteration in soybean, corn, and sunflower seed oils. Experiments were conducted using a Hypercarb column (2.1 mm × 100 mm, 5 µm) and an n-octane-isopropanol (70:30, V/V) mobile phase at a flow rate of 0.25 mL· min-1 and a column temperature of 60 °C. Detection was achieved through atmospheric pressure chemical ionization-mass spectrometry. Analysis of diverse edible oil samples revealed that oils of the same type shared similar TAG compositions, while different types exhibited distinct TAG profiles. Distinct variations in triglyceride composition were observed across different edible oils. Based on liquid chromatography‒mass spectrometry analysis, the characteristic component 1-stearic acid-2-palmitic acid-3-oleic acid glyceride (SPO), which may also include PSO, was identified in lard through principal component analysis and orthogonal partial least squares discriminant analysis. This component served as a marker for detecting as low as 0.1% lard adulteration in soybean, corn, and sunflower seed oils. The technique offers a precise and effective approach for the identification of lard adulteration in these edible oils.
期刊介绍:
Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods.
This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.