蚯蚓通过改变细菌群落对氨苄西林污染土壤中抗生素耐药基因清除的影响

IF 2.2 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Xuan Gao, Hong Zhang, Longhui Xu, Lida Wang, Xiqing Li, Yongbin Jiang, Hongmei Yu, Guoping Zhu
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引用次数: 0

摘要

抗生素抗性基因(ARGs)作为污染物出现在土壤中,对公众健康构成了严重威胁。蚯蚓(Eisenia foetida)是土壤中的常见居民,它们对 ARGs 的影响已被广泛研究。然而,蚯蚓对青霉素相关 ARGs 的具体影响仍不清楚。在本研究中,我们调查了蚯蚓在减轻氨苄西林污染土壤中的 ARGs(特别是青霉素相关 ARGs)方面的作用。利用高通量定量 PCR(HT-qPCR)技术,我们对蚯蚓处理过的土壤中青霉素相关 ARGs 的相对丰度进行了定量分析,结果表明,蚯蚓处理过的土壤中青霉素相关 ARGs 的相对丰度明显降低,p 值为 0.05。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Impact of earthworms on antibiotic resistance genes removal in ampicillin-contaminated soil through bacterial community alteration

The emergence of antibiotic resistance genes (ARGs) as contaminants in soil poses a significant threat to public health. Earthworms (Eisenia foetida), which are common inhabitants of soil, have been extensively studied for their influence on ARGs. However, the specific impact of earthworms on penicillin-related ARGs remains unclear. In this study, we investigate the role of earthworms in mitigating ARGs, specifically penicillin-related ARGs, in ampicillin-contaminated soil. Utilizing high-throughput quantitative PCR (HT-qPCR), we quantified a significant reduction in the relative abundance of penicillin-related ARGs in soil treated with earthworms, showing a decrease with a p-value of <0.01. Furthermore, high-throughput 16S rRNA gene sequencing revealed that earthworm intervention markedly alters the microbial community structure, notably enhancing the prevalence of specific bacterial phyla such as Proteobacteria, Firmicutes, Chloroflexi, and Tenericutes. Our findings not only demonstrate the effectiveness of earthworms in reducing the environmental load of penicillin-related ARGs but also provide insight into the alteration of microbial communities as a potential mechanism. This research contributes to our understanding of the role of earthworms in mitigating the spread of antibiotic resistance and provides valuable insights for the development of strategies to combat this global health issue.

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来源期刊
Journal of environmental quality
Journal of environmental quality 环境科学-环境科学
CiteScore
4.90
自引率
8.30%
发文量
123
审稿时长
3 months
期刊介绍: Articles in JEQ cover various aspects of anthropogenic impacts on the environment, including agricultural, terrestrial, atmospheric, and aquatic systems, with emphasis on the understanding of underlying processes. To be acceptable for consideration in JEQ, a manuscript must make a significant contribution to the advancement of knowledge or toward a better understanding of existing concepts. The study should define principles of broad applicability, be related to problems over a sizable geographic area, or be of potential interest to a representative number of scientists. Emphasis is given to the understanding of underlying processes rather than to monitoring. Contributions are accepted from all disciplines for consideration by the editorial board. Manuscripts may be volunteered, invited, or coordinated as a special section or symposium.
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