Andrea Rossnerova , Irena Chvojkova , Fatima Elzeinova , Daniela Pelclova , Pavlina Klusackova , Vladimir Zdimal , Lucie Ondrackova , Pavel Bradna , Adela Roubickova , Zuzana Simova , Pavel Rossner Jr.
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引用次数: 0
摘要
纳米粒子（NPs）已成为日常生活的重要组成部分，包括在牙科中的应用。除了毋庸置疑的好处之外，纳米粒子对人类健康和/或基因组的风险问题也随之而来。然而，有关细胞遗传学影响的研究却完全缺失。一组妇女在工作前后被纳米复合材料磨牙过程中释放的气溶胶急性暴露。进行了包括纳米（PM0.1）和可吸入（PM4）部分的暴露监测。对 1 号、4 号常染色体和 X 号染色体进行了全染色体绘制，以估算包括结构和数值改变在内的细胞遗传学损伤模式。结果显示，易位的基因组频率（FG/100）保持稳定，而常染色体 1 号、4 号和 X 号基因组的易位频率则高达 37.8%（p<0.05）。
Genetic alteration profiling in middle-aged women acutely exposed during the mechanical processing of dental nanocomposites
Nanoparticles (NPs) have become an important part of everyday life, including their application in dentistry. Aside from their undoubted benefits, questions regarding their risk to human health, and/or genome have arisen. However, studies concerning cytogenetic effects are completely absent. A group of women acutely exposed to an aerosol released during dental nanocomposite grinding was sampled before and after the work. Exposure monitoring including nano (PM0.1) and respirable (PM4) fractions was performed. Whole-chromosome painting for autosomes #1, #4, and gonosome X was applied to estimate the pattern of cytogenetic damage including structural and numerical alterations. The results show stable genomic frequency of translocations (FG/100), in contrast to a significant 37.8% (p<0.05) increase of numerical aberrations caused by monosomies (p<0.05), but not trisomies. Monosomies were mostly observed for chromosome X. In conclusion, exposure to nanocomposites in stomatology may lead to an increase in numerical aberrations which can be dangerous for dividing cells.
期刊介绍：
Environmental Toxicology and Pharmacology publishes the results of studies concerning toxic and pharmacological effects of (human and veterinary) drugs and of environmental contaminants in animals and man.
Areas of special interest are: molecular mechanisms of toxicity, biotransformation and toxicokinetics (including toxicokinetic modelling), molecular, biochemical and physiological mechanisms explaining differences in sensitivity between species and individuals, the characterisation of pathophysiological models and mechanisms involved in the development of effects and the identification of biological markers that can be used to study exposure and effects in man and animals.
In addition to full length papers, short communications, full-length reviews and mini-reviews, Environmental Toxicology and Pharmacology will publish in depth assessments of special problem areas. The latter publications may exceed the length of a full length paper three to fourfold. A basic requirement is that the assessments are made under the auspices of international groups of leading experts in the fields concerned. The information examined may either consist of data that were already published, or of new data that were obtained within the framework of collaborative research programmes. Provision is also made for the acceptance of minireviews on (classes of) compounds, toxicities or mechanisms, debating recent advances in rapidly developing fields that fall within the scope of the journal.
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