{"title":"从 Rhodanobacter sp.中提取的生物荧光色素 PHB 纳米颗粒的药物输送配方。","authors":"Ksheerabdhi Tanaya, Swati Mohapatra, Deviprasad Samantaray","doi":"10.1007/s10482-024-01973-x","DOIUrl":null,"url":null,"abstract":"<div><p>Biogenic nanoparticles (NPs) have emerged as promising therapeutic formulations in effective drug delivery. Despite of various positive attributes, these NPs are often conjugated with various cytotoxic organic fluorophores for bioimaging, thereby reducing its effectiveness as a potential carrier. Herein, we aim to formulate biogenic fluorescent pigmented polyhydroxybutyrate (PHB) NPs from <i>Rhodanobacter</i> sp. strain KT31 (OK001852) for drug delivery. The bacterial strain produced 0.5 g L<sup>−1</sup> of polyhydroxyalkanoates (PHAs) from 2.04 g L<sup>−1</sup> of dry cell weight (DCW) under optimised conditions via submerged fermentation. Further, structural, thermal, and morphological charactersiation of the extracted PHAs was conducted using advance analytical technologies. IR spectra at 1719.25 cm<sup>−1</sup> confirmed presence of C = O functional group PHB. NMR and XRD analysis validated the chemical structure and crystallinity of PHB. TG–DTA revealed <i>Tm</i> (168 °C), <i>Td</i> (292 °C), and <i>Xc</i> (35%) of the PHB. FE-SEM imaging indicated rough surface of the PHB film and the biodegradability was confirmed from open windro composting. WST1 assay showed no significant cell death (> 50%) from 100 to 500 µg/mL, endorsing non-cytotoxic nature of PHB. PHB NPs were uniform, smooth and spherical with size distribution and mean zeta potential 44.73 nm and 0.5 mV. IR and XRD peaks obtained at 1721.75 cm<sup>−1</sup> and 48.42 Å denoted C = O and crystalline nature of PHB. Cell proliferation rate of PHB NPs was quite significant at 50 µg/mL, establishing the non-cytotoxic nature of NPs. Further, in vitro efficacy of the PHB NPs needs to be evaluated prior to the biomedical applications.</p><h3>Graphical Abstract</h3><p>Schematic diagram depicting synthesis and characterisation of fluorescent PHB NPs</p>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":50746,"journal":{"name":"Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology","volume":"117 1","pages":""},"PeriodicalIF":1.8000,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Formulation of biogenic fluorescent pigmented PHB nanoparticles from Rhodanobacter sp. for drug delivery\",\"authors\":\"Ksheerabdhi Tanaya, Swati Mohapatra, Deviprasad Samantaray\",\"doi\":\"10.1007/s10482-024-01973-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Biogenic nanoparticles (NPs) have emerged as promising therapeutic formulations in effective drug delivery. Despite of various positive attributes, these NPs are often conjugated with various cytotoxic organic fluorophores for bioimaging, thereby reducing its effectiveness as a potential carrier. Herein, we aim to formulate biogenic fluorescent pigmented polyhydroxybutyrate (PHB) NPs from <i>Rhodanobacter</i> sp. strain KT31 (OK001852) for drug delivery. The bacterial strain produced 0.5 g L<sup>−1</sup> of polyhydroxyalkanoates (PHAs) from 2.04 g L<sup>−1</sup> of dry cell weight (DCW) under optimised conditions via submerged fermentation. Further, structural, thermal, and morphological charactersiation of the extracted PHAs was conducted using advance analytical technologies. IR spectra at 1719.25 cm<sup>−1</sup> confirmed presence of C = O functional group PHB. NMR and XRD analysis validated the chemical structure and crystallinity of PHB. TG–DTA revealed <i>Tm</i> (168 °C), <i>Td</i> (292 °C), and <i>Xc</i> (35%) of the PHB. FE-SEM imaging indicated rough surface of the PHB film and the biodegradability was confirmed from open windro composting. WST1 assay showed no significant cell death (> 50%) from 100 to 500 µg/mL, endorsing non-cytotoxic nature of PHB. PHB NPs were uniform, smooth and spherical with size distribution and mean zeta potential 44.73 nm and 0.5 mV. IR and XRD peaks obtained at 1721.75 cm<sup>−1</sup> and 48.42 Å denoted C = O and crystalline nature of PHB. Cell proliferation rate of PHB NPs was quite significant at 50 µg/mL, establishing the non-cytotoxic nature of NPs. 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引用次数: 0
摘要
生物纳米粒子(NPs)已成为有效给药的有前途的治疗制剂。尽管这些 NPs 具有各种积极的特性,但它们通常会与用于生物成像的各种细胞毒性有机荧光团共轭,从而降低了其作为潜在载体的有效性。在此,我们旨在利用罗丹菌株 KT31(OK001852)配制生物荧光色素聚羟丁酸(PHB)NPs,用于药物输送。在优化条件下,该细菌菌株通过浸没发酵从 2.04 g L-1 的干细胞重量(DCW)中产生了 0.5 g L-1 的聚羟基烷酸酯(PHA)。此外,还利用先进的分析技术对提取的 PHAs 进行了结构、热和形态特征分析。1719.25 cm-1 的红外光谱证实了 C = O 官能团 PHB 的存在。核磁共振和 XRD 分析证实了 PHB 的化学结构和结晶度。TG-DTA 显示了 PHB 的 Tm(168 °C)、Td(292 °C)和 Xc(35%)。FE-SEM 图像显示 PHB 薄膜表面粗糙,露天堆肥证实了其生物降解性。WST1 检测显示,从 100 微克/毫升到 500 微克/毫升,细胞死亡率均不明显(> 50%),这表明 PHB 无细胞毒性。PHB NPs 大小分布均匀、光滑、呈球形,平均 zeta 电位为 44.73 nm 和 0.5 mV。在 1721.75 cm-1 和 48.42 Å 处出现的 IR 和 XRD 峰表明 PHB 具有 C = O 和结晶性质。在 50 µg/mL 的浓度下,PHB NPs 的细胞增殖率非常显著,这证明了 NPs 的无毒性。此外,在生物医学应用之前,还需要对 PHB NPs 的体外功效进行评估。
Formulation of biogenic fluorescent pigmented PHB nanoparticles from Rhodanobacter sp. for drug delivery
Biogenic nanoparticles (NPs) have emerged as promising therapeutic formulations in effective drug delivery. Despite of various positive attributes, these NPs are often conjugated with various cytotoxic organic fluorophores for bioimaging, thereby reducing its effectiveness as a potential carrier. Herein, we aim to formulate biogenic fluorescent pigmented polyhydroxybutyrate (PHB) NPs from Rhodanobacter sp. strain KT31 (OK001852) for drug delivery. The bacterial strain produced 0.5 g L−1 of polyhydroxyalkanoates (PHAs) from 2.04 g L−1 of dry cell weight (DCW) under optimised conditions via submerged fermentation. Further, structural, thermal, and morphological charactersiation of the extracted PHAs was conducted using advance analytical technologies. IR spectra at 1719.25 cm−1 confirmed presence of C = O functional group PHB. NMR and XRD analysis validated the chemical structure and crystallinity of PHB. TG–DTA revealed Tm (168 °C), Td (292 °C), and Xc (35%) of the PHB. FE-SEM imaging indicated rough surface of the PHB film and the biodegradability was confirmed from open windro composting. WST1 assay showed no significant cell death (> 50%) from 100 to 500 µg/mL, endorsing non-cytotoxic nature of PHB. PHB NPs were uniform, smooth and spherical with size distribution and mean zeta potential 44.73 nm and 0.5 mV. IR and XRD peaks obtained at 1721.75 cm−1 and 48.42 Å denoted C = O and crystalline nature of PHB. Cell proliferation rate of PHB NPs was quite significant at 50 µg/mL, establishing the non-cytotoxic nature of NPs. Further, in vitro efficacy of the PHB NPs needs to be evaluated prior to the biomedical applications.
Graphical Abstract
Schematic diagram depicting synthesis and characterisation of fluorescent PHB NPs
期刊介绍:
Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.