{"title":"伊朗戈纳巴德医院转诊患者中大肠埃希菌致病基因(traT、hly、aer、pap 和 fimH)和抗生素耐药基因(blaTEM、blaSHV 和 blaCTX)的鉴定与评估。","authors":"Alireza Mohammadzadeh, Hamid Naghizadeh, Ahmad Mosadegh, Akram Astani, Omid Pouresmaeil, Jalal Mardaneh","doi":"10.61186/rbmb.12.3.465","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Urinary tract infection (UTI) is one of the common bacterial infections. <i>Escherichia coli</i> is the most common cause of UTI. In this research, the prevalence of several virulence factors and beta-lactam resistance genes was investigated.</p><p><strong>Methods: </strong>One hundred <i>E. coli</i> isolates were collected from patients' specimens with UTI referred to Allame-Bohlol Gonabadi hospital. Polymerase chain reaction (PCR) was performed to identify five pathogenic genes (<i>fimH, aer, pap, hly, traT</i>) and three antibiotic resistance genes (<i>blaTEM, blaCTX, blaSHV</i>).</p><p><strong>Results: </strong>The frequencies of <i>blaSHV, blaTEM</i> and <i>blaCTX</i> beta-lactamase genes among extended-spectrum-beta-lactamases (ESBLs) positive isolates were 11.1%, 48.1%, and 93.3%, respectively. A significant number of isolates were resistant to the most commonly used antibiotics.</p><p><strong>Conclusion: </strong>Pathogenic genes may also increase the severity, progression, and expansion of urinary tract infections. Therefore, identifying these genes as critical controllers of illness can use for better manage the treatment.</p>","PeriodicalId":45319,"journal":{"name":"Reports of Biochemistry and Molecular Biology","volume":"12 3","pages":"465-475"},"PeriodicalIF":1.6000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11015931/pdf/","citationCount":"0","resultStr":"{\"title\":\"Identification and Evaluation of Pathogenic Genes (<i>traT, hly, aer, pap,</i> and <i>fimH</i>) and Antibiotic Resistance Genes (<i>blaTEM, blaSHV,</i> and <i>blaCTX</i>) in <i>Escherichia coli</i> in Patients Referred to Gonabad Hospitals, Iran.\",\"authors\":\"Alireza Mohammadzadeh, Hamid Naghizadeh, Ahmad Mosadegh, Akram Astani, Omid Pouresmaeil, Jalal Mardaneh\",\"doi\":\"10.61186/rbmb.12.3.465\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Urinary tract infection (UTI) is one of the common bacterial infections. <i>Escherichia coli</i> is the most common cause of UTI. In this research, the prevalence of several virulence factors and beta-lactam resistance genes was investigated.</p><p><strong>Methods: </strong>One hundred <i>E. coli</i> isolates were collected from patients' specimens with UTI referred to Allame-Bohlol Gonabadi hospital. Polymerase chain reaction (PCR) was performed to identify five pathogenic genes (<i>fimH, aer, pap, hly, traT</i>) and three antibiotic resistance genes (<i>blaTEM, blaCTX, blaSHV</i>).</p><p><strong>Results: </strong>The frequencies of <i>blaSHV, blaTEM</i> and <i>blaCTX</i> beta-lactamase genes among extended-spectrum-beta-lactamases (ESBLs) positive isolates were 11.1%, 48.1%, and 93.3%, respectively. A significant number of isolates were resistant to the most commonly used antibiotics.</p><p><strong>Conclusion: </strong>Pathogenic genes may also increase the severity, progression, and expansion of urinary tract infections. Therefore, identifying these genes as critical controllers of illness can use for better manage the treatment.</p>\",\"PeriodicalId\":45319,\"journal\":{\"name\":\"Reports of Biochemistry and Molecular Biology\",\"volume\":\"12 3\",\"pages\":\"465-475\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11015931/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Reports of Biochemistry and Molecular Biology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.61186/rbmb.12.3.465\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Reports of Biochemistry and Molecular Biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.61186/rbmb.12.3.465","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Identification and Evaluation of Pathogenic Genes (traT, hly, aer, pap, and fimH) and Antibiotic Resistance Genes (blaTEM, blaSHV, and blaCTX) in Escherichia coli in Patients Referred to Gonabad Hospitals, Iran.
Background: Urinary tract infection (UTI) is one of the common bacterial infections. Escherichia coli is the most common cause of UTI. In this research, the prevalence of several virulence factors and beta-lactam resistance genes was investigated.
Methods: One hundred E. coli isolates were collected from patients' specimens with UTI referred to Allame-Bohlol Gonabadi hospital. Polymerase chain reaction (PCR) was performed to identify five pathogenic genes (fimH, aer, pap, hly, traT) and three antibiotic resistance genes (blaTEM, blaCTX, blaSHV).
Results: The frequencies of blaSHV, blaTEM and blaCTX beta-lactamase genes among extended-spectrum-beta-lactamases (ESBLs) positive isolates were 11.1%, 48.1%, and 93.3%, respectively. A significant number of isolates were resistant to the most commonly used antibiotics.
Conclusion: Pathogenic genes may also increase the severity, progression, and expansion of urinary tract infections. Therefore, identifying these genes as critical controllers of illness can use for better manage the treatment.
期刊介绍:
The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.