基于液滴的蛋白质组学发现 CD36 是乳腺基底上皮细胞祖细胞的标记物。

IF 4.3 Q1 BIOCHEMICAL RESEARCH METHODS

Cell Reports Methods Pub Date : 2024-04-22 Epub Date: 2024-04-02 DOI:10.1016/j.crmeth.2024.100741

Matthew Waas, Amanda Khoo, Pirashaanthy Tharmapalan, Curtis W McCloskey, Meinusha Govindarajan, Bowen Zhang, Shahbaz Khan, Paul D Waterhouse, Rama Khokha, Thomas Kislinger

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引用次数: 0

摘要

稀有细胞群的深度蛋白质组图谱分析一直受到样品输入要求的限制。在这里，我们介绍了 DROPPS（基于液滴的蛋白质组样品一锅制备），这是一种可访问的低投入平台，可生成 100-2500 个细胞的高保真蛋白质组图谱。通过在乳腺上皮细胞中应用 DROPPS，我们阐明了线粒体活性与克隆性之间的联系，确定了 CD36 作为基底细胞区祖细胞能力的标志物。我们预计 DROPPS 将加速对多种稀有细胞群进行生物学驱动的蛋白质组学研究。

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Droplet-based proteomics reveals CD36 as a marker for progenitors in mammary basal epithelium.

Deep proteomic profiling of rare cell populations has been constrained by sample input requirements. Here, we present DROPPS (droplet-based one-pot preparation for proteomic samples), an accessible low-input platform that generates high-fidelity proteomic profiles of 100-2,500 cells. By applying DROPPS within the mammary epithelium, we elucidated the connection between mitochondrial activity and clonogenicity, identifying CD36 as a marker of progenitor capacity in the basal cell compartment. We anticipate that DROPPS will accelerate biology-driven proteomic research for a multitude of rare cell populations.

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来源期刊

Cell Reports Methods Chemistry (General), Biochemistry, Genetics and Molecular Biology (General), Immunology and Microbiology (General)

CiteScore

3.80

自引率

0.00%

发文量

审稿时长

111 days