Fatemeh Noroozi, Masoumeh Asle-Rousta, Rahim Amini, Zeinab Sahraeian
{"title":"α-蒎烯通过靶向氧化应激、炎症和细胞凋亡，减轻了 CCl4 引起的大鼠肾脏和睾丸损伤。","authors":"Fatemeh Noroozi, Masoumeh Asle-Rousta, Rahim Amini, Zeinab Sahraeian","doi":"10.22038/IJBMS.2024.73116.15890","DOIUrl":null,"url":null,"abstract":"<p><strong>Objectives: </strong>Renal and testicular disorders are primarily associated with oxidative damage and inflammation. Here, alpha-pinene (a type of monoterpene) was investigated for its effect on oxidative/nitrosative stress and the expression of inflammatory and apoptotic factors in the kidneys and testes of rats treated with CCl<sub>4</sub>.</p><p><strong>Materials and methods: </strong>CCl<sub>4</sub> was injected intraperitoneally (IP) at a dose of 2 ml/kg (twice a week for six weeks). Alpha-pinene (50 mg/kg/day, IP) was also treated during the same period.</p><p><strong>Results: </strong>CCl<sub>4</sub> increased the level of malondialdehyde (<i>P</i><0.01 in the kidney and <i>P</i><0.001 in the testis) and nitric oxide (<i>P</i><0.001 in the kidney and <i>P</i><0.01 in the testis) and decreased the levels of glutathione (<i>P</i><0.05) in the kidneys and testicles of rats. CCl<sub>4</sub> also reduced the catalase enzyme activity in the kidneys (<i>P</i><0.05) but did not affect its activity in the testis. In addition, CCl<sub>4</sub> enhanced the mRNA expression of TNF-α (<i>P</i><0.01), nuclear factor-κB (P<0.05), and Bax (<i>P</i><0.05 in the kidney and <i>P</i><0.01 in the testis) and decreased the expression of Bcl-2 (<i>P</i><0.05) in both organs. Alpha-pinene prevented all the mentioned changes, but it did not influence the expression of Bcl-2 in the kidneys of rats receiving CCl<sub>4</sub>.</p><p><strong>Conclusion: </strong>Alpha-pinene may have the potential to prevent renal and testicular diseases by strengthening the antioxidant system in the kidneys and testis, and inhibiting oxidative/nitrosative stress, inflammation, and apoptosis caused by CCl<sub>4</sub>.</p>","PeriodicalId":14495,"journal":{"name":"Iranian Journal of Basic Medical Sciences","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11024401/pdf/","citationCount":"0","resultStr":"{\"title\":\"Alpha-pinene alleviates CCl<sub>4</sub>-induced renal and testicular injury in rats by targeting oxidative stress, inflammation, and apoptosis.\",\"authors\":\"Fatemeh Noroozi, Masoumeh Asle-Rousta, Rahim Amini, Zeinab Sahraeian\",\"doi\":\"10.22038/IJBMS.2024.73116.15890\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objectives: </strong>Renal and testicular disorders are primarily associated with oxidative damage and inflammation. Here, alpha-pinene (a type of monoterpene) was investigated for its effect on oxidative/nitrosative stress and the expression of inflammatory and apoptotic factors in the kidneys and testes of rats treated with CCl<sub>4</sub>.</p><p><strong>Materials and methods: </strong>CCl<sub>4</sub> was injected intraperitoneally (IP) at a dose of 2 ml/kg (twice a week for six weeks). Alpha-pinene (50 mg/kg/day, IP) was also treated during the same period.</p><p><strong>Results: </strong>CCl<sub>4</sub> increased the level of malondialdehyde (<i>P</i><0.01 in the kidney and <i>P</i><0.001 in the testis) and nitric oxide (<i>P</i><0.001 in the kidney and <i>P</i><0.01 in the testis) and decreased the levels of glutathione (<i>P</i><0.05) in the kidneys and testicles of rats. CCl<sub>4</sub> also reduced the catalase enzyme activity in the kidneys (<i>P</i><0.05) but did not affect its activity in the testis. In addition, CCl<sub>4</sub> enhanced the mRNA expression of TNF-α (<i>P</i><0.01), nuclear factor-κB (P<0.05), and Bax (<i>P</i><0.05 in the kidney and <i>P</i><0.01 in the testis) and decreased the expression of Bcl-2 (<i>P</i><0.05) in both organs. Alpha-pinene prevented all the mentioned changes, but it did not influence the expression of Bcl-2 in the kidneys of rats receiving CCl<sub>4</sub>.</p><p><strong>Conclusion: </strong>Alpha-pinene may have the potential to prevent renal and testicular diseases by strengthening the antioxidant system in the kidneys and testis, and inhibiting oxidative/nitrosative stress, inflammation, and apoptosis caused by CCl<sub>4</sub>.</p>\",\"PeriodicalId\":14495,\"journal\":{\"name\":\"Iranian Journal of Basic Medical Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11024401/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Basic Medical Sciences\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.22038/IJBMS.2024.73116.15890\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MEDICINE, RESEARCH & EXPERIMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Basic Medical Sciences","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.22038/IJBMS.2024.73116.15890","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}
Alpha-pinene alleviates CCl4-induced renal and testicular injury in rats by targeting oxidative stress, inflammation, and apoptosis.
Objectives: Renal and testicular disorders are primarily associated with oxidative damage and inflammation. Here, alpha-pinene (a type of monoterpene) was investigated for its effect on oxidative/nitrosative stress and the expression of inflammatory and apoptotic factors in the kidneys and testes of rats treated with CCl4.
Materials and methods: CCl4 was injected intraperitoneally (IP) at a dose of 2 ml/kg (twice a week for six weeks). Alpha-pinene (50 mg/kg/day, IP) was also treated during the same period.
Results: CCl4 increased the level of malondialdehyde (P<0.01 in the kidney and P<0.001 in the testis) and nitric oxide (P<0.001 in the kidney and P<0.01 in the testis) and decreased the levels of glutathione (P<0.05) in the kidneys and testicles of rats. CCl4 also reduced the catalase enzyme activity in the kidneys (P<0.05) but did not affect its activity in the testis. In addition, CCl4 enhanced the mRNA expression of TNF-α (P<0.01), nuclear factor-κB (P<0.05), and Bax (P<0.05 in the kidney and P<0.01 in the testis) and decreased the expression of Bcl-2 (P<0.05) in both organs. Alpha-pinene prevented all the mentioned changes, but it did not influence the expression of Bcl-2 in the kidneys of rats receiving CCl4.
Conclusion: Alpha-pinene may have the potential to prevent renal and testicular diseases by strengthening the antioxidant system in the kidneys and testis, and inhibiting oxidative/nitrosative stress, inflammation, and apoptosis caused by CCl4.
期刊介绍：
The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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