成人哮喘的气道和全身免疫球蛋白谱分析及免疫反应

IF 4.6 2区 医学 Q1 RESPIRATORY SYSTEM
Lung Pub Date : 2024-06-01 Epub Date: 2024-05-07 DOI:10.1007/s00408-024-00699-x
Laura J Walsh, Ashley Sullivan, Chris Ward, Eoin B Hunt, Susan Lapthorne, Joseph A Eustace, Liam J Fanning, Barry J Plant, Paul M O'Byrne, John A MacSharry, Desmond M Murphy
{"title":"成人哮喘的气道和全身免疫球蛋白谱分析及免疫反应","authors":"Laura J Walsh, Ashley Sullivan, Chris Ward, Eoin B Hunt, Susan Lapthorne, Joseph A Eustace, Liam J Fanning, Barry J Plant, Paul M O'Byrne, John A MacSharry, Desmond M Murphy","doi":"10.1007/s00408-024-00699-x","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Immunoglobulins play a vital role in host immune response and in the pathogenesis of conditions like asthma. Therapeutic agents such as monoclonal antibodies target specific elements of the asthmatic inflammatory cascade. Decisions to utilize these medications are often based on systemic inflammatory profiling without direct insight into the airway inflammatory profile. We sought to investigate the relationship between immunoglobulin and cytokine profiles in the airway and systemic immune compartments of adult asthmatics.</p><p><strong>Methods: </strong>Blood sampling and bronchoscopy with bronchoalveolar lavage (BAL) were performed in 76 well-defined adult asthmatics. Antibody and cytokine profiles were measured in both BAL and serum using ELISA and quantibody arrays.</p><p><strong>Results: </strong>There was no relationship between BAL and serum levels of IgE. This is of significance in an asthma population. For some analytes, correlation analysis was significant (P < 0.05) indicating representativeness of our cohort and experimental setup in those cases. Nevertheless, the predictive power (r<sup>2</sup>) of the BAL-to-serum comparisons was mostly low except for TNF-α (r<sup>2</sup> = 0.73) when assuming a simple (linear) relationship.</p><p><strong>Conclusion: </strong>This study highlights the importance of sample site when investigating the roles of immunoglobulins and cytokines in disease pathogenesis and suggests that both localized and systemic immune responses are at play. The prescription of asthma monoclonal therapy is generally based on systemic evaluation of cytokine and immunoglobulin levels. Our research suggests that this approach may not fully reflect the pathophysiology of the disease and may provide insight into why some patients respond to these targeted therapies while others do not.</p>","PeriodicalId":18163,"journal":{"name":"Lung","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11142944/pdf/","citationCount":"0","resultStr":"{\"title\":\"Airway and Systemic Immunoglobulin Profiling and Immune Response in Adult Asthma.\",\"authors\":\"Laura J Walsh, Ashley Sullivan, Chris Ward, Eoin B Hunt, Susan Lapthorne, Joseph A Eustace, Liam J Fanning, Barry J Plant, Paul M O'Byrne, John A MacSharry, Desmond M Murphy\",\"doi\":\"10.1007/s00408-024-00699-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Immunoglobulins play a vital role in host immune response and in the pathogenesis of conditions like asthma. Therapeutic agents such as monoclonal antibodies target specific elements of the asthmatic inflammatory cascade. Decisions to utilize these medications are often based on systemic inflammatory profiling without direct insight into the airway inflammatory profile. We sought to investigate the relationship between immunoglobulin and cytokine profiles in the airway and systemic immune compartments of adult asthmatics.</p><p><strong>Methods: </strong>Blood sampling and bronchoscopy with bronchoalveolar lavage (BAL) were performed in 76 well-defined adult asthmatics. Antibody and cytokine profiles were measured in both BAL and serum using ELISA and quantibody arrays.</p><p><strong>Results: </strong>There was no relationship between BAL and serum levels of IgE. This is of significance in an asthma population. For some analytes, correlation analysis was significant (P < 0.05) indicating representativeness of our cohort and experimental setup in those cases. Nevertheless, the predictive power (r<sup>2</sup>) of the BAL-to-serum comparisons was mostly low except for TNF-α (r<sup>2</sup> = 0.73) when assuming a simple (linear) relationship.</p><p><strong>Conclusion: </strong>This study highlights the importance of sample site when investigating the roles of immunoglobulins and cytokines in disease pathogenesis and suggests that both localized and systemic immune responses are at play. The prescription of asthma monoclonal therapy is generally based on systemic evaluation of cytokine and immunoglobulin levels. Our research suggests that this approach may not fully reflect the pathophysiology of the disease and may provide insight into why some patients respond to these targeted therapies while others do not.</p>\",\"PeriodicalId\":18163,\"journal\":{\"name\":\"Lung\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2024-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11142944/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Lung\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s00408-024-00699-x\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/5/7 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q1\",\"JCRName\":\"RESPIRATORY SYSTEM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lung","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s00408-024-00699-x","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/7 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"RESPIRATORY SYSTEM","Score":null,"Total":0}
引用次数: 0

摘要

引言免疫球蛋白在宿主免疫反应和哮喘等疾病的发病机制中发挥着重要作用。单克隆抗体等治疗药物以哮喘炎症级联反应的特定成分为靶点。使用这些药物的决定往往是基于全身炎症特征,而没有直接了解气道炎症特征。我们试图研究成年哮喘患者气道和全身免疫分区中免疫球蛋白和细胞因子谱之间的关系:方法:我们对 76 名定义明确的成年哮喘患者进行了血液采样、支气管镜检查和支气管肺泡灌洗(BAL)。结果:BAL 和血清中的抗体和细胞因子图谱均通过酶联免疫吸附试验(ELISA)和量子抗体阵列进行了测定:结果:BAL 和血清中的 IgE 水平之间没有关系。这在哮喘人群中意义重大。对于某些分析物,在假设简单(线性)关系的情况下,BAL 与血清之间的相关性分析具有显著性(P 2),但 TNF-α 除外(r2 = 0.73):本研究强调了在研究免疫球蛋白和细胞因子在疾病发病机制中的作用时取样部位的重要性,并表明局部和全身免疫反应都在起作用。哮喘单克隆疗法的处方通常基于对细胞因子和免疫球蛋白水平的系统评估。我们的研究表明,这种方法可能并不能完全反映疾病的病理生理学,而且可能会让人了解为什么有些患者对这些靶向疗法有反应,而有些患者却没有反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Airway and Systemic Immunoglobulin Profiling and Immune Response in Adult Asthma.

Airway and Systemic Immunoglobulin Profiling and Immune Response in Adult Asthma.

Introduction: Immunoglobulins play a vital role in host immune response and in the pathogenesis of conditions like asthma. Therapeutic agents such as monoclonal antibodies target specific elements of the asthmatic inflammatory cascade. Decisions to utilize these medications are often based on systemic inflammatory profiling without direct insight into the airway inflammatory profile. We sought to investigate the relationship between immunoglobulin and cytokine profiles in the airway and systemic immune compartments of adult asthmatics.

Methods: Blood sampling and bronchoscopy with bronchoalveolar lavage (BAL) were performed in 76 well-defined adult asthmatics. Antibody and cytokine profiles were measured in both BAL and serum using ELISA and quantibody arrays.

Results: There was no relationship between BAL and serum levels of IgE. This is of significance in an asthma population. For some analytes, correlation analysis was significant (P < 0.05) indicating representativeness of our cohort and experimental setup in those cases. Nevertheless, the predictive power (r2) of the BAL-to-serum comparisons was mostly low except for TNF-α (r2 = 0.73) when assuming a simple (linear) relationship.

Conclusion: This study highlights the importance of sample site when investigating the roles of immunoglobulins and cytokines in disease pathogenesis and suggests that both localized and systemic immune responses are at play. The prescription of asthma monoclonal therapy is generally based on systemic evaluation of cytokine and immunoglobulin levels. Our research suggests that this approach may not fully reflect the pathophysiology of the disease and may provide insight into why some patients respond to these targeted therapies while others do not.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Lung
Lung 医学-呼吸系统
CiteScore
9.10
自引率
10.00%
发文量
95
审稿时长
6-12 weeks
期刊介绍: Lung publishes original articles, reviews and editorials on all aspects of the healthy and diseased lungs, of the airways, and of breathing. Epidemiological, clinical, pathophysiological, biochemical, and pharmacological studies fall within the scope of the journal. Case reports, short communications and technical notes can be accepted if they are of particular interest.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信