Mastaneh Zeraatiannejad, Mohammad Javad Mokhtari, Afshin Borhani-Haghighi
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引用次数: 0
摘要
背景:快速诊断缺血性脑卒中(IS)是临床研究中的一个关键问题,因为它可以更有效地治疗和阻止IS的进展。中风后血液中的环状核糖核酸(CircRNAs)水平可能是一种快速诊断标志物:本研究使用实时聚合酶链反应(PCR)评估血液中的循环 RNA 水平。结果:与对照组相比,患者血液中的 circRNA DLG 相关蛋白 4(CircDLGAP4)含量降低,逻辑回归显示其表达与 IS 风险呈负相关。人类基因组版本38_Circular_0008980(hg38_circ_0008980)的表达水平在小血管疾病(SVD)患者中显著降低,线性回归分析表明hg38_circ_0008980的表达与SVD亚型呈负相关:综上所述,我们发现 IS 后 hg38_circ_0008980 水平显著下降;它可能是 IS 病理生理学中的一种新型 circRNA,与中风严重程度呈正相关。
Association of Circulating Circular RNAs (hg38_circ_0008980, and CircDLGAP4) in Diagnosis, Diseases Severity, and Prognosis of Ischemic Stroke.
Background: Fast diagnosing ischemic stroke (IS) is a critical issue in clinical studies, as it allows more effective therapy and stops the progression of IS. The blood level of circular RNAs (CircRNAs) after stroke may be a rapid diagnostic marker.
Methods: In this study, the blood level of circRNAs was evaluated using a real-time polymerase chain reaction (PCR). We used logistic and linear regression analysis to assess the potential of circRNAs levels with the risk of IS.
Results: circRNA DLG associated protein 4 (CircDLGAP4) was decreased in patients compared with controls, and logistic regression showed its expression negatively associated with IS risk. The expression level of human genome version 38_Circular_0008980 (hg38_circ_0008980) was reduced significantly in patients with small vessel disease (SVD), and the linear regression analysis showed a negative relationship between hg38_circ_0008980 expressions with SVD subtype. hg38_circ_0008980 expression relative to controls showed a significant association with IS risk.
Conclusion: Taken together, we found a significant decrease in the level of hg38_circ_0008980 after IS; it may act as a novel circRNA in IS pathophysiology with a positive correlation with stroke severity.
期刊介绍:
The Reports of Biochemistry & Molecular Biology (RBMB) is the official journal of the Varastegan Institute for Medical Sciences and is dedicated to furthering international exchange of medical and biomedical science experience and opinion and a platform for worldwide dissemination. The RBMB is a medical journal that gives special emphasis to biochemical research and molecular biology studies. The Journal invites original and review articles, short communications, reports on experiments and clinical cases, and case reports containing new insights into any aspect of biochemistry and molecular biology that are not published or being considered for publication elsewhere. Publications are accepted in the form of reports of original research, brief communications, case reports, structured reviews, editorials, commentaries, views and perspectives, letters to authors, book reviews, resources, news, and event agenda.