分离和鉴定用于降解塑料(高密度聚乙烯)的生物探究性肠道菌株 Bacillus safensis CGK192 和 Bacillus australimaris CGK221。

IF 2 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Biotechnology Letters Pub Date : 2024-08-01 Epub Date: 2024-05-06 DOI:10.1007/s10529-024-03486-z
Kamal Kant Sharma, Himalaya Panwar, Kartikey Kumar Gupta
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引用次数: 0

摘要

本研究报告了新型肠道菌株 Bacillus safensis CGK192(登录号:OM658336)和 Bacillus australimaris CGK221(登录号:OM658338)在合成聚合物(即高密度聚乙烯(HDPE))生物降解中的应用。以聚合物重量损失为基础的生物降解试验是在实验室条件下进行的,为期 90 天,同时以总蛋白含量和存活细胞数(CFU/cm2)定期评估细菌的生物量。值得注意的是,与对照组相比,两种菌株都能在不进行任何物理或化学预处理的情况下显著降低高密度聚乙烯薄膜的重量。为了评估菌株在聚合物表面形成细菌生物膜的能力,还对疏水性和生物表面活性剂进行了表征。此外,还使用傅立叶变换红外光谱(FTIR)、X 射线衍射(XRD)、场发射扫描电子显微镜(FE-SEM)和能量色散 X 射线(EDX)以及气相色谱-质谱联用仪(GC-MS)等分析技术对高密度聚乙烯进行了降解后表征,以确认降解情况。有趣的是,与菌株 CGK192 相比,菌株 CGK221 在聚合物表面形成生物膜的效率更高,半衰期更短(即 0.00032 天-1),羰基指数更高。这些发现反映出我们的菌株能够形成生物膜,并在聚合物表面引入含氧官能团,从而使聚合物更容易降解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and characterization of bio-prospecting gut strains Bacillus safensis CGK192 and Bacillus australimaris CGK221 for plastic (HDPE) degradation.

The present work reports the application of novel gut strains Bacillus safensis CGK192 (Accession No. OM658336) and Bacillus australimaris CGK221 (Accession No. OM658338) in the biological degradation of synthetic polymer i.e., high-density polyethylene (HDPE). The biodegradation assay based on polymer weight loss was conducted under laboratory conditions for a period of 90 days along with regular evaluation of bacterial biomass in terms of total protein content and viable cells (CFU/cm2). Notably, both strains achieved significant weight reduction for HDPE films without any physical or chemical pretreatment in comparison to control. Hydrophobicity and biosurfactant characterization were also done in order to assess strains ability to form bacterial biofilm over the polymer surface. The post-degradation characterization of HDPE was also performed to confirm degradation using analytical techniques such as Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Field emission scanning electronic microscopy (FE-SEM) coupled with energy dispersive X-ray (EDX), and Gas chromatography-mass spectrometry (GC-MS). Interestingly strain CGK221 was found to be more efficient in forming biofilm over polymer surface as indicated by lower half-life (i.e., 0.00032 day-1) and higher carbonyl index in comparison to strain CGK192. The findings reflect the ability of our strains to develop biofilm and introduce an oxygenic functional group into the polymer surface, thereby making it more susceptible to degradation.

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来源期刊
Biotechnology Letters
Biotechnology Letters 工程技术-生物工程与应用微生物
CiteScore
5.90
自引率
3.70%
发文量
108
审稿时长
1.2 months
期刊介绍: Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.
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