姜酮通过氧化应激、内质网应激、炎症、细胞凋亡和自噬途径对亚砷酸钠诱导的大鼠睾丸毒性的影响

IF 2.1 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
Sibel Çiğdem Tuncer, Cihan Gur, Sefa Kucukler, Serkan Ali Akarsu, Fatih Mehmet Kandemir
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引用次数: 0

摘要

研究目的本研究旨在探讨姜酮(ZNG)治疗对亚砷酸钠(SA)诱导的大鼠睾丸毒性的影响:研究共分为五组(n=7),实验组分别为:车辆组、ZNG 组、SA 组、SA+ZNG 25 组和 SA+ZNG 50 组。大鼠口服 SA 的剂量为 10 毫克/千克/体重,口服 ZNG 的剂量为 25 毫克/千克/体重和 50 毫克/千克/体重,连续 14 天:结果:本研究观察到,给大鼠口服 SA 后,其睾丸组织中的 MDA 含量增加,而 GSH 含量、SOD、CAT 和 GPx 活性显著降低。施用 SA 后,促炎症基因 NF-κB、TNF-α、IL-1β 和 IL-6 的 mRNA 转录水平被触发。此外,施用 SA 会增加 RAGE、NLRP3 和 JAK-2/STAT3 基因的表达,从而引起炎症。此外,在 SA 处理的大鼠睾丸组织中还出现了内质网(ER)应激,从而导致 ATF-6、PERK、IRE1 和 GRP78 基因上调。SA 通过上调睾丸中 Bax 和 Caspase-3 的表达以及抑制 Bcl-2 的表达而导致细胞凋亡。SA引起睾丸组织学异常,导致精子质量下降:结论:ZNG治疗可降低SA诱导的氧化应激、ER应激、炎症、细胞凋亡和睾丸组织学异常,同时提高精子质量。因此,ZNG 可以减轻 SA 对睾丸的毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of zingerone on rat induced testicular toxicity by sodium arsenite via oxidative stress, endoplasmic reticulum stress, inflammation, apoptosis, and autophagy pathways.

Objectives: This study aimed to investigate the effects of zingerone (ZNG) treatment on testicular toxicity in rats induced by sodium arsenite (SA).

Materials and methods: In the study, five groups were formed (n=7) and the experimental groups were designated as follows; Vehicle group, ZNG group, SA group, SA+ZNG 25 group, and SA+ZNG 50 group. While SA was administered orally to rats at 10 mg/kg/bw, ZNG was given to rats orally at 25 and 50 mg/kg/bw doses for 14 days.

Results: As a result of the presented study, an increase was observed in the MDA contents of the testicular tissue of the rats administered SA, while significant decreases were observed in GSH levels, SOD, CAT, and GPx activities. The mRNA transcript levels of the pro-inflammatory genes NF-κB, TNF-α, IL-1β, and IL-6 were triggered after SA administration. Additionally, SA administration caused inflammation by increasing RAGE, NLRP3, and JAK-2/STAT3 gene expression. Moreover, endoplasmic reticulum (ER) stress occurred in the testicular tissues of SA-treated rats and thus ATF-6, PERK, IRE1, and GRP78 genes were up-regulated. SA caused apoptosis by up-regulating Bax and Caspase-3 expressions and inhibiting Bcl-2 expression in testicles. SA caused histological irregularities in the testicles, resulting in decreased sperm quality.

Conclusion: ZNG treatment reduced SA-induced oxidative stress, ER stress, inflammation, apoptosis, and histological irregularities in the testicles while increasing sperm quality. As a result, it was observed that ZNG could alleviate the toxicity caused by SA in the testicles.

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来源期刊
Iranian Journal of Basic Medical Sciences
Iranian Journal of Basic Medical Sciences MEDICINE, RESEARCH & EXPERIMENTAL-PHARMACOLOGY & PHARMACY
CiteScore
4.00
自引率
4.50%
发文量
142
审稿时长
6-12 weeks
期刊介绍: The Iranian Journal of Basic Medical Sciences (IJBMS) is a peer-reviewed, monthly publication by Mashhad University of Medical Sciences (MUMS), Mashhad, Iran . The Journal of "IJBMS” is a modern forum for scientific communication. Data and information, useful to investigators in any discipline in basic medical sciences mainly including Anatomical Sciences, Biochemistry, Genetics, Immunology, Microbiology, Pathology, Pharmacology, Pharmaceutical Sciences, and Physiology, will be published after they have been peer reviewed. This will also include reviews and multidisciplinary research.
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