利用天然深共晶溶剂介导的亚临界水水解作用从虾废料中回收蛋白质和多糖,用于生物降解薄膜。

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Md Sadek Ali, Vikash Chandra Roy, Jin-Seok Park, Ahmed Redwan Haque, Jin Hong Mok, Wei Zhang, Byung-Soo Chun
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引用次数: 0

摘要

由于塑料和虾壳在户外的不当处置,环境污染已成为一个重大问题。因此,利用废弃材料合成可生物降解薄膜就显得尤为重要。本研究的新颖之处在于从虾壳中提取蛋白质水解物和壳聚糖,并用这些材料制成生物可降解薄膜。本研究采用溶液浇铸法制备了复合薄膜。此外,还研究了超声波预处理(UPT)和天然深共晶溶剂(NADES)作为萃取介质的综合效果,以确定它们对虾废料亚临界水水解(SWH)的潜在影响。虾壳在 NADES 溶液中进行 UPT,然后在 150 至 230 °C 的不同温度和 3 兆帕的压力下进行 20 分钟的 SWH。然后,对水解物的理化性质和生物活性进行评估,以确定其是否适合用于生物降解包装膜。此外,还对所得水解物的理化性质和生物活性进行了分析。在 190 °C/UPT/NADES 条件下,蛋白质含量最高(391.96 ± 0.48 mg BSA/g),不同种类的肽的蛋白质分子的平均分子大小均小于 1000 Da。总体而言,UPT 和 SWH 联合处理比单独处理的抗氧化活性水平更高。最后,通过包裹鱼类样本并评估其脂质氧化情况,对复合膜的应用进行了评估。使用较高浓度的蛋白水解物可以明显延缓样品的变化,从而证明了薄膜的适用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Protein and Polysaccharide Recovery from Shrimp Wastes by Natural Deep Eutectic Solvent Mediated Subcritical Water Hydrolysis for Biodegradable Film

Protein and Polysaccharide Recovery from Shrimp Wastes by Natural Deep Eutectic Solvent Mediated Subcritical Water Hydrolysis for Biodegradable Film

Environmental pollution is a significant problem due to the improper disposal of plastics and shrimp shells outdoors. Therefore, the synthesis of biodegradable film from waste materials is highly important. The novelty of this research lies in the extraction of protein hydrolysates and chitosan from shrimp shells, as well as the fabrication of biodegradable film from these materials. In this study, the composite films were produced using the solution casting method. Moreover, the combined effect of ultrasound pretreatments (UPT) and natural deep eutectic solvents (NADES) was investigated as extraction media, to determine their potential impact on shrimp waste subcritical water hydrolysis (SWH). Shrimp shells were submitted to UPT in NADES solution, followed by SWH at different temperatures ranging from 150 to 230 °C under 3 MPa for 20 min. Then, the physiochemical properties and bioactivities of the hydrolysates were assessed to determine their suitability for use in biodegradable packaging films. Additionally, the physiochemical properties and bioactivities of the resulting hydrolysates were also analyzed. The highest amount of protein (391.96 ± 0.48 mg BSA/g) was obtained at 190 °C/UPT/NADES, and the average molecular size of the protein molecules was less than 1000 Da with different kinds of peptide. Overall, combined UPT and SWH treatments yielded higher antioxidant activity levels than individual treatments. Finally, the application of composite films was evaluated by wrapping fish samples and assessing their lipid oxidation. The use of higher concentrations of protein hydrolysates significantly delayed changes in the samples, thereby demonstrating the film’s applicability.

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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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