直接提取和测定卷烟烟雾中的游离尼古丁

IF 2.3 3区化学 Q3 CHEMISTRY, ANALYTICAL

Journal of Analytical Methods in Chemistry Pub Date : 2024-05-04 DOI:10.1155/2024/9273705

Li Li, Jing Wen, Yuyang Deng, Juan Yang, Yue Yuan, Yi Shen, Guoce Liu, Yonghong Tian, Dean Lei

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引用次数: 0

摘要

准确测定卷烟烟气中的游离尼古丁含量对于评估卷烟质量、研究危害和成瘾性以及降低焦油含量至关重要。目前，烟草制品中游离尼古丁的测定主要依靠 pH 值计算、核磁共振（NMR）光谱、顶空固相微萃取（HS-SPME）和传统溶剂萃取等方法。然而，这些方法都存在局限性，限制了其广泛应用。本研究按照传统的溶剂萃取方法，使用环己烷直接萃取卷烟烟气中的游离尼古丁，并通过气相色谱-质谱法进行检测。与传统的两相溶剂萃取法相比，我们的实验方法简便易行，消除了水溶液对烟气颗粒物中尼古丁原始分布的影响。此外，烟草中质子化尼古丁的存在也不会影响测定结果。与 HS-SPME 和 NMR 光谱法相比，我们的方法是先进行溶剂萃取，然后进行色谱分离和仪器检测，因此操作简单、精度高、检测限更高，而且减少了仪器检测阶段的干扰。实验中标准添加回收率为 96.2% 至 102.5%。检测限为 2.8 微克/支，标准曲线的二次回归相关系数超过 0.999。平行样品的相对标准偏差在 1.7% 至 3.4% 之间（n = 5），完全符合测定卷烟中游离尼古丁的要求。对 38 个市售品牌卷烟样品的分析表明，游离尼古丁的含量范围为 0.376 至 0.716 毫克/支，平均为 0.540 毫克/支，游离尼古丁占尼古丁总含量的 39.1%-88.8%。

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Direct Extraction and Determination of Free Nicotine in Cigarette Smoke

The accurate determination of the free nicotine content in cigarette smoke is crucial for assessing cigarette quality, studying harm and addiction, and reducing tar levels. Currently, the determination of free nicotine in tobacco products primarily relies on methods such as pH calculation, nuclear magnetic resonance (NMR) spectroscopy, headspace solid-phase microextraction (HS-SPME), and traditional solvent extraction. However, these methods have limitations that restrict their widespread application. In this study, the free nicotine in cigarette smoke was directly extracted by using cyclohexane according to the traditional solvent extraction method and detected via gas chromatography-mass spectrometry. Compared with the traditional two-phase solvent extraction, our experimental method is easy to execute and eliminates the influence of aqueous solutions on the original distribution of nicotine in cigarette smoke particulate matter. Furthermore, the presence of protonated nicotine in tobacco does not affect the determination. Compared with HS-SPME and NMR spectroscopy, our approach, which involves solvent extraction followed by chromatographic separation and instrumental detection, offers simplicity, improved precision, better detection limits, and reduced interference during the instrumental detection stage. The standard addition recoveries in the conducted experiment ranged from 96.2% to 102.5%. The limit of detection was 2.8 μg/cig, and the correlation coefficient for the quadratic regression of the standard curve exceeded 0.999. The relative standard deviation for parallel samples was between 1.7% and 3.4% (n = 5), fully meeting the requirements for the determination of free nicotine in cigarette smoke. Analysis of cigarette samples from 38 commercially available brands revealed that the content of free nicotine ranged from 0.376 to 0.716 mg/cig, with an average of 0.540 mg/cig, and free nicotine accounted for 39.1%–88.8% of the total nicotine content.

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来源期刊

Journal of Analytical Methods in Chemistry CHEMISTRY, ANALYTICAL-ENGINEERING, CIVIL

CiteScore

4.80

自引率

3.80%

发文量

审稿时长

6-12 weeks

期刊介绍： Journal of Analytical Methods in Chemistry publishes papers reporting methods and instrumentation for chemical analysis, and their application to real-world problems. Articles may be either practical or theoretical. Subject areas include (but are by no means limited to): Separation Spectroscopy Mass spectrometry Chromatography Analytical Sample Preparation Electrochemical analysis Hyphenated techniques Data processing As well as original research, Journal of Analytical Methods in Chemistry also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.