SIT1 可识别系统性红斑狼疮患者中细胞毒性分子分泌增加的循环低活性 T 细胞

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Ainizati Hasimu, Ayibaota Bahabayi, Ziqi Xiong, Qi Li, Zhonghui Zhang, Xingyue Zeng, Mohan Zheng, Zihang Yuan, Chen Liu
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引用次数: 0

摘要

本研究旨在阐明 SIT1 在人体循环 CD8/CD4 + T 细胞中的表达和功能,并明确其在系统性红斑狼疮(SLE)患者中的意义。我们采用多参数流式细胞术研究了循环 CD8/CD4 + T 细胞及其各自亚群中 SIT1 的表达,并对健康对照组(HCs)和系统性红斑狼疮患者进行了比较。此外,我们还评估了健康对照组和系统性红斑狼疮患者在 PMA 刺激前后的 SIT1 相关 CD8/CD4 + T 细胞中颗粒酶 B、穿孔素、IL-17 和 IFN-γ 的水平。在临床上,我们进行了接收者操作特征曲线分析和相关性分析,以评估 SIT1 相关 CD8/CD4 + T 细胞在系统性红斑狼疮患者中的临床意义。SIT1 在 CD4 + T 细胞中的表达量较高,与 SIT1 + T 细胞相比,SIT1 - T 细胞的颗粒酶 B、穿孔素和 IFN-γ 水平升高。与未受刺激的 T 细胞相比,受 PMA 刺激的 T 细胞的 SIT1 表达减少。系统性红斑狼疮患者的 CD8 + T 细胞中 SIT1 + 比例增加,而 SIT1 + CD4 + T 细胞数量减少。此外,与 HCs 相比,系统性红斑狼疮患者的 SIT1 + 细胞表现出明显更高的颗粒酶 B 和穿孔素水平。SIT1 + 细胞与系统性红斑狼疮患者的临床指标有明显关联,与 SIT1 相关的指标在系统性红斑狼疮患者的诊断中很有价值。SIT1 与 T 细胞活化成反比。在系统性红斑狼疮患者中,随着细胞毒性分子分泌的增加,T 细胞中的 SIT1 表达也发生了改变。这种上调可能有助于系统性红斑狼疮的发病机制并提高其诊断潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

SIT1 identifies circulating hypoactive T cells with elevated cytotoxic molecule secretion in systemic lupus erythematosus patients

SIT1 identifies circulating hypoactive T cells with elevated cytotoxic molecule secretion in systemic lupus erythematosus patients

This study aims to elucidate the expression and functionality of SIT1 in circulating CD8/CD4 + T cells in humans and to delineate its significance in systemic lupus erythematosus (SLE) patients. We employed multiparametric flow cytometry to investigate the expression of SIT1 in circulating CD8/CD4 + T cells and their respective subsets, comparing healthy controls (HCs) with SLE patients. Furthermore, we assessed the levels of granzyme B, perforin, IL-17, and IFN-γ in SIT1-related CD8/CD4 + T cells from both HCs and SLE patients, both before and after PMA stimulation. Clinically, we conducted receiver operating characteristic curve analysis and correlation analysis to evaluate the clinical relevance of SIT1-related CD8/CD4 + T cells in SLE patients. SIT1 exhibited higher expression in CD4 + T cells, with SIT1 − T cells demonstrating elevated levels of granzyme B, perforin, and IFN-γ compared to SIT1 + T cells. PMA-stimulated T cells exhibited reduced SIT1 expression compared to unstimulated T cells. SLE patients displayed increased SIT1 + proportions in CD8 + T cells and decreased SIT1 + CD4 + T cell numbers. Additionally, SIT1 + cells in SLE patients exhibited significantly higher levels of granzyme B and perforin compared to HCs. SIT1 + cells demonstrated significant associations with clinical indicators in SLE patients, with indicators related to SIT1 proving valuable in the diagnosis of SLE patients. SIT1 is inversely correlated with T cell activation. In SLE patients, SIT1 expression is altered in T cells concomitant with an augmented secretion of cytotoxic molecules. This upregulation may contribute to the pathogenesis of SLE and enhance its diagnostic potential.

Graphical abstract

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CiteScore
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