miR-221-3p 在急性肺栓塞并发肺动脉高压中上调,并通过抑制 PTEN 促进肺动脉平滑肌细胞增殖和迁移

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Lei Tang, Shuai Niu, Jinwei Xu, Wei Lu, Li Zhou
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引用次数: 0

摘要

肺动脉平滑肌细胞(PASMCs)的功能与肺动脉高压(PH)的发病机制有关,而肺动脉高压是急性肺栓塞(APE)的一种危及生命的并发症。本研究旨在探讨微RNA(miR)-221-3p在APE-PH患者中的表达模式及其在PASMC增殖和迁移中的作用。研究收集了 APE-PH 患者的临床资料和静脉血。测定血清中miR-221-3p和磷酸酶与天丝蛋白同源物(PTEN)的表达水平,然后对miR-221-3p的诊断效果进行受体运算特征曲线分析。用 miR-221-3p mimics 和 PTEN 表达载体转染 PASMC,然后通过细胞计数试剂盒-8、5-乙炔基-2′-脱氧尿苷、Transwell 和伤口愈合试验评估细胞活力、增殖和迁移。miR-221-3p与PTEN 3′UTR区域的结合通过双荧光素酶检测得到证实。miR-221在APE-PH患者的血清中上调,具有良好的诊断效果,临界值为1.155,敏感性为66.25%,特异性为67.50%。在 APE-PH 患者中,miR-221 与 PTEN 呈负相关。miR-221 的过表达促进了体外 PASMCs 的增殖和迁移。PTEN的过量表达消除了miR-221-3p在PASMCs中的促进作用。总之,miR-221-3p靶向PTEN促进了PASMC的增殖和迁移。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

miR-221-3p is upregulated in acute pulmonary embolism complicated with pulmonary hypertension and promotes pulmonary arterial smooth muscle cells proliferation and migration by inhibiting PTEN

miR-221-3p is upregulated in acute pulmonary embolism complicated with pulmonary hypertension and promotes pulmonary arterial smooth muscle cells proliferation and migration by inhibiting PTEN

Pulmonary arterial smooth muscle cells (PASMCs) functions are associated with the pathogenesis of pulmonary hypertension (PH) which is a life-threatening complication of acute pulmonary embolism (APE). This study sought to explore the expression pattern of microRNA (miR)-221-3p in APE-PH patients and its role in PASMCs proliferation and migration. The clinical data and venous blood of APE-PH patients were collected. The expression levels of miR-221-3p and phosphatase and tensin homolog (PTEN) in serum were determined, followed by receiver operator characteristic curve analysis of miR-221-3p diagnostic efficacy. PASMCs were transfected with miR-221-3p mimics and PTEN-overexpressed vector, followed by assessment of cell viability, proliferation, and migration through cell counting kit-8, 5‐ethynyl‐2′‐deoxyuridine, Transwell, and wound healing assays. The binding between miR-221-3p and PTEN 3′UTR region was testified by the dual-luciferase assay. miR-221 was upregulated in the serum of APE-PH patients and presented with good diagnostic efficacy with 1.155 cutoff value, 66.25% sensitivity, and 67.50% specificity. miR-221 was negatively correlated with PTEN in APE-PH patients. miR-221 overexpression facilitated PASMCs proliferation and migration in vitro. miR-221-3p bound to PTEN 3′UTR region to decrease PTEN protein levels. PTEN overexpression abolished the promotive role of miR-221-3p in PASMCs. Overall, miR-221-3p targeted PTEN to facilitate PASMC proliferation and migration.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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