HBV 相关肝病中 10 种循环细胞因子/趋化因子的表达情况

IF 3.1 2区医学 Q3 IMMUNOLOGY

Infectious Agents and Cancer Pub Date : 2024-05-01 DOI:10.1186/s13027-024-00580-9

Yanfang Jia, Xiaolei Jiao, Wenxia Shi, Ying Luo, Huiling Xiang, Jing Liang, Yingtang Gao

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引用次数: 0

摘要

细胞因子/凝血因子在乙型肝炎病毒（HBV）感染的发生和发展过程中起着至关重要的作用。本研究旨在观察 10 种相关细胞因子/造血因子在健康人、自限性患者和不同疾病阶段（慢性乙型肝炎（CHB）、肝硬化（LC）、肝细胞增生异常结节（DNs）和肝细胞癌（HCC））的 HBV 感染者血清中的表达模式，并分析这些细胞因子/造血因子与疾病进展的关系。研究采用 Luminex 多路复用技术对六种细胞因子（FGF-2、IFN-α2、IL-4、IL-6、IL-10 和 VEGF-A）和四种趋化因子（GRO-α、IL-8、IP-10 和 MCP-1）的水平进行了量化。10 种细胞因子/趋化因子的表达在健康人和自限性患者之间无明显差异。CHB组和LC组的IL-4、IL-6和IL-8水平明显升高。IL-10在HCC组中表达量较高。所有肝病组（CHB、LC、DN 和 HCC）的 IP-10 水平均明显高于 HI 和 SL-HBV 组，而所有肝病组的 GRO 水平均明显低于 HI 和 SL-HBV 组。术前组和术后两天组的 10 种细胞因子/凝血因子水平无明显差异。术后 3 个月，观察到 IL-4、VEGF-A 和 IL-8 水平明显升高，IL-10 和 GRO-α 水平明显降低。相关性分析表明，大多数相关性差异显著的细胞因子/凝血因子在 HCC 手术前后呈正相关。我们的研究结果突显了特定细胞因子在 HBV 感染相关疾病进展中的波动状态。据推测，这些细胞因子可作为血清标志物，用于监测 HBV 相关肝病进展过程中的动态变化，并预测患者的预后。

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Expression of 10 circulating cytokines/chemokines in HBV-related liver disease

Cytokines/chemokines play essential roles in the occurrence and progression of hepatitis B virus (HBV) infection. This study aimed to observe the expression patterns of 10 related cytokines/chemokines in the serum of healthy individuals, self-limited patients and HBV-infected patients at different stages of disease (chronic hepatitis B (CHB), liver cirrhosis (LC), hepatocellular dysplastic nodules (DNs) and hepatocellular carcinoma (HCC)) and to analyze the relationships of these cytokines/chemokines with disease progression. The levels of six cytokines (FGF-2, IFN-α2, IL-4, IL-6, IL-10 and VEGF-A) and four chemokines (GRO-α, IL-8, IP-10 and MCP-1) were quantified using Luminex multiplex technology. There were no significant differences in the expression of the 10 cytokines/chemokines between healthy individuals and self-limited patients. The levels of IL-4, IL-6, and IL-8 increased significantly in the CHB and LC groups. IL-10 was highly expressed in the HCC group. The level of IP-10 was significantly greater in all liver disease groups (CHB, LC, DN and HCC) than in the HI and SL-HBV groups, while the level of GRO was significantly lower in all liver disease groups than in the HI and SL-HBV groups. The levels of the 10 cytokines/chemokines were not significantly different between the preoperative group and the two-day postoperative group. Significant increases in the levels of IL-4, VEGF-A and IL-8 and significant decreases in those of IL-10 and GRO-α were observed 3 months after surgery. Correlation analysis revealed that most of the cytokines/chemokines with significant correlation differences were positively correlated before and after HCC surgery. Our results highlight the fluctuating status of specific cytokines in HBV infection-related disease progression. It is speculated that these cytokines may be used as serum markers to monitor dynamic changes during the progression of HBV-related liver disease and to predict patient prognosis.

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来源期刊

Infectious Agents and Cancer ONCOLOGY-IMMUNOLOGY

CiteScore

5.80

自引率

2.70%

发文量

期刊介绍： Infectious Agents and Cancer is an open access, peer-reviewed online journal that encompasses all aspects of basic, clinical, epidemiological and translational research providing an insight into the association between chronic infections and cancer. The journal welcomes submissions in the pathogen-related cancer areas and other related topics, in particular: • HPV and anogenital cancers, as well as head and neck cancers; • EBV and Burkitt lymphoma; • HCV/HBV and hepatocellular carcinoma as well as lymphoproliferative diseases; • HHV8 and Kaposi sarcoma; • HTLV and leukemia; • Cancers in Low- and Middle-income countries. The link between infection and cancer has become well established over the past 50 years, and infection-associated cancer contribute up to 16% of cancers in developed countries and 33% in less developed countries. Preventive vaccines have been developed for only two cancer-causing viruses, highlighting both the opportunity to prevent infection-associated cancers by vaccination and the gaps that remain before vaccines can be developed for other cancer-causing agents. These gaps are due to incomplete understanding of the basic biology, natural history, epidemiology of many of the pathogens that cause cancer, the mechanisms they exploit to cause cancer, and how to interrupt progression to cancer in human populations. Early diagnosis or identification of lesions at high risk of progression represent the current most critical research area of the field supported by recent advances in genomics and proteomics technologies.