PVT-1和miR-29a/29b的表达作为肝硬化的可靠生物标志物及其与炎症生物标志物谱的相关性。

IF 2.7 4区医学 Q3 TOXICOLOGY

Human & Experimental Toxicology Pub Date : 2024-04-30 DOI:10.1177/09603271241251451

Shaza E. Khalaf, Shima N. Abdelfattah, Amal K. Khaliefa, Sahar A. Daoud, Enas Yahia, Nabil A. Hasona

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引用次数: 0

摘要

背景& 目的肝脏是负责众多新陈代谢过程的重要器官，长非编码RNA（lncRNA）和microRNA（miRNA）可对这些过程产生重大影响。这些核糖核酸（RNA）分子已被证明在调节基因表达方面起着至关重要的作用，它们的失调与许多肝脏疾病有关。我们的研究旨在探讨浆细胞瘤变异易位-1（PVT-1）、microRNA-29a/29b（miR-29a/miR-29b）和炎症生物标志物[白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、转化生长因子-β（TGF-β）和胰岛素生长因子-1（IGF-1）]作为肝硬化诊断和预后生物标志物的诊断准确性。因此，了解 lncRNAs 和 miRNAs 影响肝脏代谢的机制对于开发有效治疗肝脏相关疾病的方法至关重要。方法收集了 164 名参与者的血清样本，其中包括 114 名不同等级的肝硬化患者（35 名 I 级、35 名 II 级和 44 名 III 级）和 50 名健康对照者。通过反转录-定量聚合酶链反应（RT-PCR）分析了PVT-1和miR-29a/miR-29b的表达，同时使用酶联免疫吸附测定法（ELISA）评估了血清中炎症生物标志物的水平。ROC分析表明，PVT-1和miR-29a/miR-29b在区分肝硬化患者和健康对照组方面具有很好的鉴别力。我们的研究结果还加强了循环炎症生物标志物（IL-6、TNF-α、TGF-β 和 IGF-1）水平在肝硬化筛查中的诊断价值。

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Expression of PVT-1 and miR-29a/29b as reliable biomarkers for liver cirrhosis and their correlation with the inflammatory biomarkers profile.

Background & AimsThe liver is a vital organ responsible for numerous metabolic processes, which can be significantly impacted by long non-coding RNAs (lncRNAs) and microRNAs (miRNAs). These ribonucleic acid (RNA) molecules have been shown to play a crucial role in regulating gene expression, and their dysregulation has been implicated in numerous liver disorders. Our study aimed to investigate the diagnostic accuracy of plasmacytoma variant translocation-1 (PVT-1), microRNA-29a/29b (miR-29a/miR-29b), and inflammatory biomarkers [ interleukine-6 (IL-6), tumor necrosis factor-alpha (TNF-α), transforming growth factor-beta (TGF-β), and insulin growth factor-1 (IGF-1)] as diagnostic and prognostic biomarkers for liver cirrhosis. Therefore, understanding the mechanisms by which lncRNAs and miRNAs influence liver metabolism is of paramount importance in developing effective treatments for liver-related diseases.MethodsSerum samples were collected from 164 participants, comprising 114 cirrhotic patients with varying grades (35 grade I, 35 grade II, and 44 grade III) and 50 healthy controls. PVT-1 and miR-29a/miR-29b expression was analyzed by reverse transcription-quantitative polymerase chain reaction (RT-PCR), while the serum levels of inflammatory biomarkers were assessed using enzyme-linked immunosorbent assay (ELISA).ResultsThe study participants exhibited notable differences in PVT-1 and miR-29a/miR-29b expression. ROC analysis revealed excellent discriminative power for PVT-1 and miR-29a/miR-29b in distinguishing cirrhotic patients from healthy controls.ConclusionThis study demonstrates the promising potential of PVT-1 and miR-29a/miR-29b as early diagnostic biomarkers for liver cirrhosis detection, requiring further validation in larger cohorts. Our findings also reinforce the diagnostic value of circulating inflammatory biomarkers (IL-6, TNF-α, TGF-β, and IGF-1) levels for liver cirrhosis screening.

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来源期刊

Human & Experimental Toxicology 医学-毒理学

CiteScore

5.70

自引率

3.60%

发文量

128

审稿时长

2.3 months

期刊介绍： Human and Experimental Toxicology (HET), an international peer reviewed journal, is dedicated to publishing preclinical and clinical original research papers and in-depth reviews that comprehensively cover studies of functional, biochemical and structural disorders in toxicology. The principal aim of the HET is to publish timely high impact hypothesis driven scholarly work with an international scope. The journal publishes on: Structural, functional, biochemical, and molecular effects of toxic agents; Studies that address mechanisms/modes of toxicity; Safety evaluation of novel chemical, biotechnologically-derived products, and nanomaterials for human health assessment including statistical and mechanism-based approaches; Novel methods or approaches to research on animal and human tissues (medical and veterinary patients) investigating functional, biochemical and structural disorder; in vitro techniques, particularly those supporting alternative methods