Laura Ratinho, Laurent Bacri, Bénédicte Thiebot*, Benjamin Cressiot* and Juan Pelta*,
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引用次数: 0
摘要
迄今为止,由于蛋白质/肽对映体的质量和电荷完全相同,还没有快速、低成本和直接的技术来识别和检测它们。由于 l 型和 d 型蛋白质对映体具有独特的构象,因此它们具有不同的生物活性,因此这种技术至关重要。对映体具有诊断多种疾病或正常身体功能的潜力,但尚未得到利用。这项研究利用气溶胶纳米孔和电学检测技术来鉴定与不同生物过程和病理相关的血管加压素对映体--l-AVP 和 d-AVP。我们根据它们在原生或还原条件下的构象,利用其特定的电学特征对它们进行了识别。为了更好地识别它们,我们使用了主成分分析方法来确定与识别它们最相关的电学参数。最后,我们使用蒙特卡罗预测法将每种事件类型分配给特定的 l- 或 d-AVP 对映异构体。
Identification and Detection of a Peptide Biomarker and Its Enantiomer by Nanopore
Until now, no fast, low-cost, and direct technique exists to identify and detect protein/peptide enantiomers, because their mass and charge are identical. They are essential since l- and d-protein enantiomers have different biological activities due to their unique conformations. Enantiomers have potential for diagnostic purposes for several diseases or normal bodily functions but have yet to be utilized. This work uses an aerolysin nanopore and electrical detection to identify vasopressin enantiomers, l-AVP and d-AVP, associated with different biological processes and pathologies. We show their identification according to their conformations, in either native or reducing conditions, using their specific electrical signature. To improve their identification, we used a principal component analysis approach to define the most relevant electrical parameters for their identification. Finally, we used the Monte Carlo prediction to assign each event type to a specific l- or d-AVP enantiomer.
An aerolysin nanopore allows the identification of vasopressin enantiomers as well as their conformation. A Monte Carlo prediction assigns each event type to a specific l- or d-AVP enantiomer.
期刊介绍:
ACS Central Science publishes significant primary reports on research in chemistry and allied fields where chemical approaches are pivotal. As the first fully open-access journal by the American Chemical Society, it covers compelling and important contributions to the broad chemistry and scientific community. "Central science," a term popularized nearly 40 years ago, emphasizes chemistry's central role in connecting physical and life sciences, and fundamental sciences with applied disciplines like medicine and engineering. The journal focuses on exceptional quality articles, addressing advances in fundamental chemistry and interdisciplinary research.