{"title":"Vepris afzelii根部水提取物对醋酸环丙孕酮诱导的大鼠性腺功能减退症的壮阳和雄激素作用","authors":"Modeste Wankeu-Nya, Ornéla Néely Djeumeni, Zacharie Nde, Marie Claire Tchamadeu, Tomutou Inès Kengne, Towo Dominique Hyacinthe Hatho, Brice Landry Koloko, Lembè Dieudonné Massoma, Alain Bertrand Dongmo, Fewou Paul Moundipa, Pierre Watcho","doi":"10.1038/s41443-024-00892-9","DOIUrl":null,"url":null,"abstract":"<p>This work aimed to evaluate the effects of the aqueous extract of <i>Vepris afzelii</i> roots on a rat model of hypogonadism. Phytochemical screening and acute toxicity of the extract were performed using different procedures. Hypogonadism was induced orally in adult Wistar rats using cyproterone acetate (30 mg/kg) for ten days. Besides six normal rats (10 ml/kg of distilled water, normal control), 30 hypogonadal rats were subdivided into five groups of six animals each, receiving for 14 days: distilled water (10 ml/kg, hypogonadal control), testosterone (4 mg/kg/3days) and the extract of <i>V. afzelii</i> (100, 200 and 400 mg/kg). Sexual behavior, sperm parameters, testes function and structure were assessed. Compared to the normal controls, significant (<i>p</i> = 0.0000) increases in mount (24 ± 0.94 seconds vs. 1200 ± 00 seconds) and intromission (49.16 ± 10.85 seconds vs. 1200 ± 00 seconds) latencies, and post-ejaculatory interval (381.72 ± 37.55 seconds vs. 1200 ± 00 seconds) were observed in all groups receiving cyproterone acetate on day 0. Total inhibitions of mounts (63.50 ± 8.91 vs. 00 ± 00), intromissions (36.66 ± 3.51 vs. 00 ± 00) (<i>p</i> = 0.0000), ejaculations (2.83 ± 00 vs. 00 ± 00, <i>p</i> = 0.0002) frequencies and mean copulatory interval (627.30 ± 81.80 vs. 00 ± 00, <i>p</i> = 0.0000) were also observed in these groups. Moreover, decreases in daily sperm production (2.65 ± 0.19 vs. 1.17 ± 0.08, <i>p</i> = 0.0498), percentage of sperm mobility (78.64 ± 8.41 vs. 10.12 ± 2.32), serum testosterone level (8.39 ± 0.63 ng/dl vs. 1.68 ± 0.19 ng/dl), diameter of seminiferous tubules (111.97 ± 0.51 µm vs. 94.51 ± 0.57 µm) and height of germinal epithelium (46.58 ± 0.34 µm vs. 33.74 ± 0.66 µm) (p = 0.0000) associated with increases in sperm transit (3.13 ± 0.45 vs. 11.07 ± 1.45, <i>p</i> = 0.0000) were also observed in these groups. Interestingly, compared to hypogonadal control and day 0, the administration of <i>V. afzelii</i> extract induced significant (<i>p</i> = 0.0000) improvements in all these altered parameters with 400 mg/kg being the most active dose. These results, attributed to saponins, flavonoids, polyphenols and triterpenes detected in this plant’s extract confirm its traditional usage and could be useful for the management of patients suffering from hypogonadism.</p><figure></figure>","PeriodicalId":14068,"journal":{"name":"International Journal of Impotence Research","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Aphrodisiac and androgenic effects of the aqueous extract of the roots of Vepris afzelii on cyproterone acetate-induced hypogonadism in rat\",\"authors\":\"Modeste Wankeu-Nya, Ornéla Néely Djeumeni, Zacharie Nde, Marie Claire Tchamadeu, Tomutou Inès Kengne, Towo Dominique Hyacinthe Hatho, Brice Landry Koloko, Lembè Dieudonné Massoma, Alain Bertrand Dongmo, Fewou Paul Moundipa, Pierre Watcho\",\"doi\":\"10.1038/s41443-024-00892-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>This work aimed to evaluate the effects of the aqueous extract of <i>Vepris afzelii</i> roots on a rat model of hypogonadism. Phytochemical screening and acute toxicity of the extract were performed using different procedures. Hypogonadism was induced orally in adult Wistar rats using cyproterone acetate (30 mg/kg) for ten days. Besides six normal rats (10 ml/kg of distilled water, normal control), 30 hypogonadal rats were subdivided into five groups of six animals each, receiving for 14 days: distilled water (10 ml/kg, hypogonadal control), testosterone (4 mg/kg/3days) and the extract of <i>V. afzelii</i> (100, 200 and 400 mg/kg). Sexual behavior, sperm parameters, testes function and structure were assessed. Compared to the normal controls, significant (<i>p</i> = 0.0000) increases in mount (24 ± 0.94 seconds vs. 1200 ± 00 seconds) and intromission (49.16 ± 10.85 seconds vs. 1200 ± 00 seconds) latencies, and post-ejaculatory interval (381.72 ± 37.55 seconds vs. 1200 ± 00 seconds) were observed in all groups receiving cyproterone acetate on day 0. Total inhibitions of mounts (63.50 ± 8.91 vs. 00 ± 00), intromissions (36.66 ± 3.51 vs. 00 ± 00) (<i>p</i> = 0.0000), ejaculations (2.83 ± 00 vs. 00 ± 00, <i>p</i> = 0.0002) frequencies and mean copulatory interval (627.30 ± 81.80 vs. 00 ± 00, <i>p</i> = 0.0000) were also observed in these groups. Moreover, decreases in daily sperm production (2.65 ± 0.19 vs. 1.17 ± 0.08, <i>p</i> = 0.0498), percentage of sperm mobility (78.64 ± 8.41 vs. 10.12 ± 2.32), serum testosterone level (8.39 ± 0.63 ng/dl vs. 1.68 ± 0.19 ng/dl), diameter of seminiferous tubules (111.97 ± 0.51 µm vs. 94.51 ± 0.57 µm) and height of germinal epithelium (46.58 ± 0.34 µm vs. 33.74 ± 0.66 µm) (p = 0.0000) associated with increases in sperm transit (3.13 ± 0.45 vs. 11.07 ± 1.45, <i>p</i> = 0.0000) were also observed in these groups. Interestingly, compared to hypogonadal control and day 0, the administration of <i>V. afzelii</i> extract induced significant (<i>p</i> = 0.0000) improvements in all these altered parameters with 400 mg/kg being the most active dose. These results, attributed to saponins, flavonoids, polyphenols and triterpenes detected in this plant’s extract confirm its traditional usage and could be useful for the management of patients suffering from hypogonadism.</p><figure></figure>\",\"PeriodicalId\":14068,\"journal\":{\"name\":\"International Journal of Impotence Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.8000,\"publicationDate\":\"2024-04-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Impotence Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1038/s41443-024-00892-9\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"UROLOGY & NEPHROLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Impotence Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1038/s41443-024-00892-9","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"UROLOGY & NEPHROLOGY","Score":null,"Total":0}
引用次数: 0
摘要
这项研究旨在评估 Vepris afzelii 根的水提取物对大鼠性腺功能减退症模型的影响。采用不同的程序对提取物进行了植物化学筛选和急性毒性研究。用醋酸环丙孕酮(30 毫克/千克)诱导成年 Wistar 大鼠性腺功能减退症,连续十天。除了 6 只正常大鼠(10 毫升/千克蒸馏水,正常对照组)外,30 只性腺功能减退大鼠被分成 5 组,每组 6 只,分别接受蒸馏水(10 毫升/千克,性腺功能减退对照组)、睾酮(4 毫克/千克/3 天)和 V. afzelii 提取物(100、200 和 400 毫克/千克)治疗 14 天。对性行为、精子参数、睾丸功能和结构进行了评估。与正常对照组相比,在第 0 天接受醋酸环丙孕酮治疗的所有组中,都观察到勃起(24 ± 0.94 秒 vs. 1200 ± 00 秒)和射精(49.16 ± 10.85 秒 vs. 1200 ± 00 秒)潜伏期和射精后间歇(381.72 ± 37.55 秒 vs. 1200 ± 00 秒)显著增加(p = 0.0000)。在这些组别中还观察到骑乘(63.50 ± 8.91 vs. 00 ± 00)、插入(36.66 ± 3.51 vs. 00 ± 00)(p = 0.0000)、射精(2.83 ± 00 vs. 00 ± 00,p = 0.0002)频率和平均交配间隔(627.30 ± 81.80 vs. 00 ± 00,p = 0.0000)的总抑制。此外,日精子产量(2.65 ± 0.19 vs. 1.17 ± 0.08,p = 0.0498)、精子活动率(78.64 ± 8.41 vs. 10.12 ± 2.32)、血清睾酮水平(8.39 ± 0.63 ng/dl vs. 1.68 ± 0.19 ng/dl)、曲细精管直径(111.97 ± 0.51 µm vs. 94.51 ± 0.57 µm)和生精上皮的高度(46.58 ± 0.34 µm vs. 33.74 ± 0.66 µm)(p = 0.0000)(p = 0.0000),同时还观察到这些组中精子转运量的增加(3.13 ± 0.45 vs. 11.07 ± 1.45,p = 0.0000)。有趣的是,与性腺功能低下的对照组和第 0 天相比,服用 V. afzelii 提取物可显著(p = 0.0000)改善所有这些改变的参数,其中 400 毫克/千克是最有效的剂量。这些结果归功于在这种植物的提取物中检测到的皂苷、黄酮类、多酚和三萜类化合物,证实了它的传统用途,可用于治疗性腺功能减退症患者。
Aphrodisiac and androgenic effects of the aqueous extract of the roots of Vepris afzelii on cyproterone acetate-induced hypogonadism in rat
This work aimed to evaluate the effects of the aqueous extract of Vepris afzelii roots on a rat model of hypogonadism. Phytochemical screening and acute toxicity of the extract were performed using different procedures. Hypogonadism was induced orally in adult Wistar rats using cyproterone acetate (30 mg/kg) for ten days. Besides six normal rats (10 ml/kg of distilled water, normal control), 30 hypogonadal rats were subdivided into five groups of six animals each, receiving for 14 days: distilled water (10 ml/kg, hypogonadal control), testosterone (4 mg/kg/3days) and the extract of V. afzelii (100, 200 and 400 mg/kg). Sexual behavior, sperm parameters, testes function and structure were assessed. Compared to the normal controls, significant (p = 0.0000) increases in mount (24 ± 0.94 seconds vs. 1200 ± 00 seconds) and intromission (49.16 ± 10.85 seconds vs. 1200 ± 00 seconds) latencies, and post-ejaculatory interval (381.72 ± 37.55 seconds vs. 1200 ± 00 seconds) were observed in all groups receiving cyproterone acetate on day 0. Total inhibitions of mounts (63.50 ± 8.91 vs. 00 ± 00), intromissions (36.66 ± 3.51 vs. 00 ± 00) (p = 0.0000), ejaculations (2.83 ± 00 vs. 00 ± 00, p = 0.0002) frequencies and mean copulatory interval (627.30 ± 81.80 vs. 00 ± 00, p = 0.0000) were also observed in these groups. Moreover, decreases in daily sperm production (2.65 ± 0.19 vs. 1.17 ± 0.08, p = 0.0498), percentage of sperm mobility (78.64 ± 8.41 vs. 10.12 ± 2.32), serum testosterone level (8.39 ± 0.63 ng/dl vs. 1.68 ± 0.19 ng/dl), diameter of seminiferous tubules (111.97 ± 0.51 µm vs. 94.51 ± 0.57 µm) and height of germinal epithelium (46.58 ± 0.34 µm vs. 33.74 ± 0.66 µm) (p = 0.0000) associated with increases in sperm transit (3.13 ± 0.45 vs. 11.07 ± 1.45, p = 0.0000) were also observed in these groups. Interestingly, compared to hypogonadal control and day 0, the administration of V. afzelii extract induced significant (p = 0.0000) improvements in all these altered parameters with 400 mg/kg being the most active dose. These results, attributed to saponins, flavonoids, polyphenols and triterpenes detected in this plant’s extract confirm its traditional usage and could be useful for the management of patients suffering from hypogonadism.
期刊介绍:
International Journal of Impotence Research: The Journal of Sexual Medicine addresses sexual medicine for both genders as an interdisciplinary field. This includes basic science researchers, urologists, endocrinologists, cardiologists, family practitioners, gynecologists, internists, neurologists, psychiatrists, psychologists, radiologists and other health care clinicians.