铜绿假单胞菌的高风险克隆污染了法国城市的饮用水网络

IF 10.4 1区 工程技术 Q1 ENGINEERING, CHEMICAL
Ani Horikian, Audrey Jeanvoine, Abdallah Amarache, Morgane Tourtet, Jérôme Ory, Hélène Boulestreau, Nathalie Van der Mee Marquet, Nadine Lemaitre, Matthieu Eveillard, Didier Lepelletier, Xavier Bertrand, Benoît Valot, Didier Hocquet
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引用次数: 0

摘要

铜绿假单胞菌(Pseudomonas aeruginosa)是一种主要的机会性病原体,可导致免疫力低下患者的严重感染。这种病原体对饮用水网(DWN)的污染被低估了,因为它大多处于持续存在的细胞状态,无法被推荐的监测技术检测到。我们采集了八个相距较远的城市的水样,并采用一种基于培养的方法来寻找铜绿假单胞菌,这种方法可以复苏宿主细胞。对分离物的基因组进行了测序。在检测的 8 个 DWN 中,有 5 个(62.5%)受到铜绿假单胞菌污染,其中 4 个受到高风险克隆(ST308、ST395)的污染。令人惊讶的是,从 4 个独立且距离较远的 DWN 中分离出的 ST308 菌株是克隆型的。大多数铜绿假单胞菌分离物共享一个基因组岛,赋予其对铜离子的耐受性。收集到的菌群结构可能既来自于水管供应的共同污染源,也来自于对共享遗传因子的克隆的选择,这些遗传因子可能有助于它们在 DWN 中的繁殖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

High-risk clones of Pseudomonas aeruginosa contaminate the drinking water networks of French cities

High-risk clones of Pseudomonas aeruginosa contaminate the drinking water networks of French cities

High-risk clones of Pseudomonas aeruginosa contaminate the drinking water networks of French cities
Pseudomonas aeruginosa is a major opportunistic pathogen responsible for severe infections in immunocompromised patients. The contamination of drinking water networks (DWNs) with this pathogen is underestimated, as it is mostly in the state of persister cells undetected by the recommended monitoring technique. We collected water samples from eight cities distant from each other and searched for P. aeruginosa using a culture-based method that resuscitates persister cells. The genomes of isolates were sequenced. Five DWNs of the eight tested (62.5%) were contaminated with P. aeruginosa, of which four were contaminated with high-risk clones (ST308, ST395). Surprisingly, the ST308 isolates retrieved from the four independent and distant DWNs were clonal. Most P. aeruginosa isolates shared a genomic island conferring tolerance to copper-ions. The population structure of the collection may result from both a common source of contamination by plumbing supplies and the selection of clones sharing genetic elements that presumably aided their propagation in DWNs.
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来源期刊
npj Clean Water
npj Clean Water Environmental Science-Water Science and Technology
CiteScore
15.30
自引率
2.60%
发文量
61
审稿时长
5 weeks
期刊介绍: npj Clean Water publishes high-quality papers that report cutting-edge science, technology, applications, policies, and societal issues contributing to a more sustainable supply of clean water. The journal's publications may also support and accelerate the achievement of Sustainable Development Goal 6, which focuses on clean water and sanitation.
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