T. Zibar Belasic , M. Badnjevic , M. Zigante , B. Mohar Vitezic , S. Spalj , E.P. Markova-Car
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Changes of the bacteria in the biofilm were analyzed by the quantitative polymerase chain reaction</p></div><div><h3>Results</h3><p>Increase in the plaque index, pH of biofilm, and WSL was observed during orthodontic treatment with standard oral hygiene. Large interindividual variability was present, and the effects of one-month use of fluorides and CHX on clinical parameters were not significant. Despite standard hygiene the abundance of studied biofilm bacteria increased - the most <em>Streptoccocus mutans</em> (14.2x) and <em>S. salivarius</em> (3.3x), moderate <em>Veillonella parvula</em> (3x) and the least <em>S. sobrinus</em> (2.3x) and <em>Agregatibacter actinomycetemcomitans</em> (1.9x). The use of CHX reduced <em>S. sobrinus</em> (2.2x) and <em>A. actinomycetemcomitans</em> (1.9x). Fluoride use reduced <em>A. actinomycetemcomitans</em> (1.3x) and <em>S. sobrinus</em> (1.2x). Fluorides better controlled <em>S. mutans</em> than CHX.</p></div><div><h3>Conclusion</h3><p>Bacterial biomass in supragingival biofilm increased during treatment with metal orthodontic appliances, with greater increase in cariogenic bacteria than periopathogens. Fluoride controlled <em>S. mutans</em>, while CHX <em>S. sobrinus</em> and <em>A. actinomycetemcomitans</em>.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"164 ","pages":"Article 105984"},"PeriodicalIF":2.2000,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Supragingival dental biofilm profile and biofilm control during orthodontic treatment with fixed orthodontic appliance: A randomized controlled trial\",\"authors\":\"T. Zibar Belasic , M. Badnjevic , M. Zigante , B. Mohar Vitezic , S. Spalj , E.P. 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引用次数: 0
摘要
目的 分析正畸治疗期间龈上牙龈生物膜控制的有效性以及细菌状况的变化。 设计 64 名年龄在 12-22 岁之间的参与者(57% 为女性)参加了这项研究。参加者使用固定矫治器进行正畸治疗,并被随机分配到三组中的一组:(I)使用洗必泰二氯葡萄糖酸盐(CHX),(II)使用高浓度氟化物(F)凝胶,(III)进行标准口腔卫生。对牙菌斑和牙龈炎指数、生物膜的 pH 值和白斑病变(WSL)进行了评估。通过定量聚合酶链反应分析了生物膜中细菌的变化。结果在使用标准口腔卫生方法进行正畸治疗期间,观察到牙菌斑指数、生物膜 pH 值和白斑病变(WSL)均有所上升。个体之间的差异很大,使用一个月的氟化物和 CHX 对临床参数的影响并不显著。尽管采取了标准的口腔卫生措施,但所研究的生物膜细菌的数量还是有所增加--最多的是变异链球菌(14.2 倍)和唾液酸链球菌(3.3 倍),中等的是副龈维龙菌(3 倍),最少的是梭状芽孢杆菌(2.3 倍)和放线菌(1.9 倍)。使用 CHX 可减少 S. sobrinus(2.2 倍)和 A. actinomycetemcomitans(1.9 倍)。使用氟化物可减少放线菌(1.3 倍)和 S. sobrinus(1.2 倍)。结论在金属正畸矫治器治疗期间,龈上牙龈生物膜中的细菌生物量增加,致龋细菌比周病原菌增加更多。氟化物可控制 S. mutans,而 CHX 可控制 S. sobrinus 和 A. actinomycetemcomitans。
Supragingival dental biofilm profile and biofilm control during orthodontic treatment with fixed orthodontic appliance: A randomized controlled trial
Objective
The effectiveness of supragingival dental biofilm control during orthodontic treatment and changes in the bacterial profile were analyzed.
Design
Sixty-four participants aged 12–22 years (57% female) were included in the study. Participants underwent orthodontic treatment with fixed appliances and were randomly assigned to one of the three groups, which during a period of one month: (I) used chlorhexidine digluconate (CHX), (II) used high concentration of fluoride (F) gel and (III) performed standard oral hygiene. The plaque and gingivitis index, pH of biofilm and white spot lesions (WSL) were assessed. Changes of the bacteria in the biofilm were analyzed by the quantitative polymerase chain reaction
Results
Increase in the plaque index, pH of biofilm, and WSL was observed during orthodontic treatment with standard oral hygiene. Large interindividual variability was present, and the effects of one-month use of fluorides and CHX on clinical parameters were not significant. Despite standard hygiene the abundance of studied biofilm bacteria increased - the most Streptoccocus mutans (14.2x) and S. salivarius (3.3x), moderate Veillonella parvula (3x) and the least S. sobrinus (2.3x) and Agregatibacter actinomycetemcomitans (1.9x). The use of CHX reduced S. sobrinus (2.2x) and A. actinomycetemcomitans (1.9x). Fluoride use reduced A. actinomycetemcomitans (1.3x) and S. sobrinus (1.2x). Fluorides better controlled S. mutans than CHX.
Conclusion
Bacterial biomass in supragingival biofilm increased during treatment with metal orthodontic appliances, with greater increase in cariogenic bacteria than periopathogens. Fluoride controlled S. mutans, while CHX S. sobrinus and A. actinomycetemcomitans.
期刊介绍:
Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including:
Cell and molecular biology
Molecular genetics
Immunology
Pathogenesis
Cellular microbiology
Embryology
Syndromology
Forensic dentistry