探索疟原虫表面蛋白,设计高免疫原性的恶性疟原虫多表位亚单位疫苗

IF 3.5 Q3 Biochemistry, Genetics and Molecular Biology
Preshita Bhalerao , Satyendra Singh , Vijay Kumar Prajapati , Tarun Kumar Bhatt
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引用次数: 0

摘要

背景疟疾数十年来一直是热带和亚热带国家居民的主要健康问题。恶性疟原虫是导致严重疟疾的重要寄生虫之一,也是造成严重死亡的主要原因。此外,这种寄生虫对世界卫生组织推荐的所有药物和疗法都产生了抗药性。因此,迫切需要以可靠疫苗的形式采取预防措施,以实现无疟疾世界的目标。表面蛋白是亚单位疫苗开发的首选,因为它们能被宿主免疫细胞和疫苗诱导抗体快速检测和参与。此外,丰富的表面或膜蛋白可能有助于疫苗诱导抗体对病原体的疏松作用。结果 在我们的研究中,我们列出了文献中所有可能对疟原虫感染和免疫逃避具有重要功能且必不可少的表面蛋白。我们列出了前红细胞期和红细胞期的 8 种疟原虫表面蛋白和膜蛋白。利用免疫信息学工具预测了这些蛋白的 37 个表位(B 细胞、CTL 和 HTL 表位),并将其与合适的肽连接物连接起来设计了疫苗构建物。在多表位系列的 N 端添加了 TLR-4 激动剂肽佐剂,然后是 PADRE 序列和 EAAAK 连接器。TLR-4 受体与构建物的预期模型结构进行了对接。在模拟生理环境下,疫苗和 TLR-4 的能量最低的复合物 -1514 是稳定的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exploring malaria parasite surface proteins to devise highly immunogenic multi-epitope subunit vaccine for Plasmodium falciparum

Background

Malaria has remained a major health concern for decades among people living in tropical and sub-tropical countries. Plasmodium falciparum is one of the critical species that cause severe malaria and is responsible for major mortality. Moreover, the parasite has generated resistance against all WHO recommended drugs and therapies. Therefore, there is an urgent need for preventive measures in the form of reliable vaccines to achieve the target of a malaria-free world. Surface proteins are the preferable choice for subunit vaccine development because they are rapidly detected and engaged by host immune cells and vaccination-induced antibodies. Additionally, abundant surface or membrane proteins may contribute to the opsonization of pathogens by vaccine-induced antibodies.

Results

In our study, we have listed all those surface proteins from the literature that could be functionally important and essential for infection and immune evasion of the malaria parasite. Eight Plasmodium surface and membrane proteins from the pre-erythrocyte and erythrocyte stages were shortlisted. Thirty-seven epitopes (B-cell, CTL, and HTL epitopes) from these proteins were predicted using immune-informatic tools and joined with suitable peptide linkers to design a vaccine construct. A TLR-4 agonist peptide adjuvant was added at the N-terminus of the multi-epitope series, followed by the PADRE sequence and EAAAK linker. The TLR-4 receptor was docked with the construct’s anticipated model structure. The complex of vaccine and TLR-4, with the lowest energy −1514, was found to be stable under simulated physiological settings.

Conclusion

This study has provided a novel multi-epitope construct that may be exploited further for the development of an efficient vaccine for malaria.

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来源期刊
Journal of Genetic Engineering and Biotechnology
Journal of Genetic Engineering and Biotechnology Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
5.70
自引率
5.70%
发文量
159
审稿时长
16 weeks
期刊介绍: Journal of genetic engineering and biotechnology is devoted to rapid publication of full-length research papers that leads to significant contribution in advancing knowledge in genetic engineering and biotechnology and provide novel perspectives in this research area. JGEB includes all major themes related to genetic engineering and recombinant DNA. The area of interest of JGEB includes but not restricted to: •Plant genetics •Animal genetics •Bacterial enzymes •Agricultural Biotechnology, •Biochemistry, •Biophysics, •Bioinformatics, •Environmental Biotechnology, •Industrial Biotechnology, •Microbial biotechnology, •Medical Biotechnology, •Bioenergy, Biosafety, •Biosecurity, •Bioethics, •GMOS, •Genomic, •Proteomic JGEB accepts
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