Victor Gomes de Paula , Rayane Sarafim de Sousa , Rafaella Christina Moreira Rocha da Silva , Everton Giovani Alves , Alexandre Rodrigues Caetano , Patrícia Ianella , Tatiana Amabile de Campos
{"title":"fim3-24/ptxP-3 基因型与巴西中西部的百日咳疫情有关:疫苗免疫驱动的百日咳杆菌菌株选择","authors":"Victor Gomes de Paula , Rayane Sarafim de Sousa , Rafaella Christina Moreira Rocha da Silva , Everton Giovani Alves , Alexandre Rodrigues Caetano , Patrícia Ianella , Tatiana Amabile de Campos","doi":"10.1016/j.meegid.2024.105599","DOIUrl":null,"url":null,"abstract":"<div><p>Whopping cough (or Pertussis) is an acute infectious respiratory disease caused by <em>Bordetella pertussis</em> bacteria. The disease is highly transmissible and can be fatal in children under two years old. Since the introduction of vaccine immunization in 1940, Pertussis incidence decreased worldwide. In Brazil, the immunization was introduced in 1977 using the whole cell (wP) vaccine. Despite the high vaccination coverage, an unexpected increase in the number of observed Pertussis cases was observed in 2012. In this year, 2257 cases were reported exceeding the average incidence rate of <1000 cases per year until 2010. This outbreak reached a peak level in 2014 and ended in 2018 according to the Brazilian National Surveillance System (SINAN). To understand the relationship between the outbreak and the vaccination, bacterial isolates (<em>n</em> = 136) from the Brazilian Midwest region obtained during the outbreak were submitted to genotyping of two vaccine loci: <em>ptx</em>P and <em>fim</em>3. Most of isolates (102) were obtained from nursing children (29 days to 2 years old). Genotyping of 94 isolates revealed that <em>fim</em>3–24/<em>ptx</em>P-3 was the most prevalent genotype (68%) associated with the outbreak peak. Two additional genotypes were also observed: <em>fim</em>3–1/<em>ptx</em>P-3 (15%) and <em>fim3</em>–3/<em>ptx</em>P-3 (17%). Conversely, the <em>fim</em>3–1/<em>ptx</em>P-2 genotype, which is harbored by the strain used in the wP vaccine (Bp137), was not observed. These results showed that <em>B. pertussis</em> circulating strains in the outbreak analyzed were different from the strain used for Pertussis immunization in Brazil. These observations provide insights that could be used to target vaccination programs to prevent future whooping cough outbreaks in Brazil.</p></div>","PeriodicalId":54986,"journal":{"name":"Infection Genetics and Evolution","volume":null,"pages":null},"PeriodicalIF":2.6000,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1567134824000509/pdfft?md5=5575f966c2ef631471d1dc56ef1dc0af&pid=1-s2.0-S1567134824000509-main.pdf","citationCount":"0","resultStr":"{\"title\":\"fim3–24/ptxP-3 genotype is associated to whooping cough outbreak in Brazilian Midwest: The selection of Bordetella pertussis strains driven by vaccine immunization\",\"authors\":\"Victor Gomes de Paula , Rayane Sarafim de Sousa , Rafaella Christina Moreira Rocha da Silva , Everton Giovani Alves , Alexandre Rodrigues Caetano , Patrícia Ianella , Tatiana Amabile de Campos\",\"doi\":\"10.1016/j.meegid.2024.105599\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Whopping cough (or Pertussis) is an acute infectious respiratory disease caused by <em>Bordetella pertussis</em> bacteria. The disease is highly transmissible and can be fatal in children under two years old. Since the introduction of vaccine immunization in 1940, Pertussis incidence decreased worldwide. In Brazil, the immunization was introduced in 1977 using the whole cell (wP) vaccine. Despite the high vaccination coverage, an unexpected increase in the number of observed Pertussis cases was observed in 2012. In this year, 2257 cases were reported exceeding the average incidence rate of <1000 cases per year until 2010. This outbreak reached a peak level in 2014 and ended in 2018 according to the Brazilian National Surveillance System (SINAN). To understand the relationship between the outbreak and the vaccination, bacterial isolates (<em>n</em> = 136) from the Brazilian Midwest region obtained during the outbreak were submitted to genotyping of two vaccine loci: <em>ptx</em>P and <em>fim</em>3. Most of isolates (102) were obtained from nursing children (29 days to 2 years old). Genotyping of 94 isolates revealed that <em>fim</em>3–24/<em>ptx</em>P-3 was the most prevalent genotype (68%) associated with the outbreak peak. Two additional genotypes were also observed: <em>fim</em>3–1/<em>ptx</em>P-3 (15%) and <em>fim3</em>–3/<em>ptx</em>P-3 (17%). Conversely, the <em>fim</em>3–1/<em>ptx</em>P-2 genotype, which is harbored by the strain used in the wP vaccine (Bp137), was not observed. These results showed that <em>B. pertussis</em> circulating strains in the outbreak analyzed were different from the strain used for Pertussis immunization in Brazil. These observations provide insights that could be used to target vaccination programs to prevent future whooping cough outbreaks in Brazil.</p></div>\",\"PeriodicalId\":54986,\"journal\":{\"name\":\"Infection Genetics and Evolution\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-04-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S1567134824000509/pdfft?md5=5575f966c2ef631471d1dc56ef1dc0af&pid=1-s2.0-S1567134824000509-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Infection Genetics and Evolution\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1567134824000509\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"INFECTIOUS DISEASES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Infection Genetics and Evolution","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1567134824000509","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}
fim3–24/ptxP-3 genotype is associated to whooping cough outbreak in Brazilian Midwest: The selection of Bordetella pertussis strains driven by vaccine immunization
Whopping cough (or Pertussis) is an acute infectious respiratory disease caused by Bordetella pertussis bacteria. The disease is highly transmissible and can be fatal in children under two years old. Since the introduction of vaccine immunization in 1940, Pertussis incidence decreased worldwide. In Brazil, the immunization was introduced in 1977 using the whole cell (wP) vaccine. Despite the high vaccination coverage, an unexpected increase in the number of observed Pertussis cases was observed in 2012. In this year, 2257 cases were reported exceeding the average incidence rate of <1000 cases per year until 2010. This outbreak reached a peak level in 2014 and ended in 2018 according to the Brazilian National Surveillance System (SINAN). To understand the relationship between the outbreak and the vaccination, bacterial isolates (n = 136) from the Brazilian Midwest region obtained during the outbreak were submitted to genotyping of two vaccine loci: ptxP and fim3. Most of isolates (102) were obtained from nursing children (29 days to 2 years old). Genotyping of 94 isolates revealed that fim3–24/ptxP-3 was the most prevalent genotype (68%) associated with the outbreak peak. Two additional genotypes were also observed: fim3–1/ptxP-3 (15%) and fim3–3/ptxP-3 (17%). Conversely, the fim3–1/ptxP-2 genotype, which is harbored by the strain used in the wP vaccine (Bp137), was not observed. These results showed that B. pertussis circulating strains in the outbreak analyzed were different from the strain used for Pertussis immunization in Brazil. These observations provide insights that could be used to target vaccination programs to prevent future whooping cough outbreaks in Brazil.
期刊介绍:
(aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID)
Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance.
However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors.
Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases.
Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .