穗状病毒蛋白 RBD 中的糖基化位点可降低 PEDV AH2012/12 的免疫原性

IF 2.5 4区医学 Q3 VIROLOGY

Virus research Pub Date : 2024-05-02 DOI:10.1016/j.virusres.2024.199381

Gege Zhang , Qi Peng , Shiyu Liu , Baochao Fan , Chuanhong Wang , Xu Song , Qiuxia Cao , Chengcheng Li , Hong Xu , Hongting Lu , Meiying Bao , Shanshan Yang , Yunchuan Li , Jiaxiang Wang , Bin Li

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引用次数: 0

摘要

猪流行性腹泻（PED）是由猪流行性腹泻病毒（PEDV）引起的一种高度传染性猪肠道疾病。接种疫苗是预防和控制猪流行性腹泻的有效策略。以往的研究证实，糖基化可调节病毒抗原的免疫原性。在本研究中，我们构建了三种去除了 RBD 中糖基化位点的重组 PEDV。病毒感染试验表明，重组病毒与亲本PEDV具有相似的复制特性。尽管动物挑战性研究表明RBD中的糖基化位点不会影响PEDV的致病性，但我们发现去除RBD区域的糖基化位点可以提高体内的IgG和中和滴度，这表明RBD中的去糖基化可以增强PEDV的免疫原性。这些研究结果表明，去除RBD上的糖基化位点是开发PEDV疫苗的一种可行方法。

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The glycosylation sites in RBD of spike protein attenuate the immunogenicity of PEDV AH2012/12

Porcine epidemic diarrhea (PED) is a highly contagious swine intestinal disease caused by PED virus (PEDV). Vaccination is a promising strategy to prevent and control PED. Previous studies have confirmed that glycosylation could regulate the immunogenicity of viral antigens. In this study, we constructed three recombinant PEDVs which removed the glycosylation sites in RBD. Viral infection assays revealed that similar replication characteristics between the recombinant viruses and parental PEDV. Although animal challenging study demonstrated that the glycosylation sites in RBD do not affect the pathogenicity of PEDV, we found that removing the glycosylation sites on the RBD regions could promote the IgG and neutralization titer in vivo, suggesting deglycosylation in RBD could enhance the immunogenicity of PEDV. These findings demonstrated that removal of the glycosylation sites in RBD is a promising method to develop PEDV vaccines.

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来源期刊

Virus research 医学-病毒学

CiteScore

9.50

自引率

2.00%

发文量

239

审稿时长

43 days

期刊介绍： Virus Research provides a means of fast publication for original papers on fundamental research in virology. Contributions on new developments concerning virus structure, replication, pathogenesis and evolution are encouraged. These include reports describing virus morphology, the function and antigenic analysis of virus structural components, virus genome structure and expression, analysis on virus replication processes, virus evolution in connection with antiviral interventions, effects of viruses on their host cells, particularly on the immune system, and the pathogenesis of virus infections, including oncogene activation and transduction.