对受到口腔微生物挑战的牙龈细胞进行光生物调节

IF 8.3 2区 材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY
J. Tanum, H.E. Kim, S.M. Lee, A. Kim, J. Korostoff, G. Hwang
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引用次数: 0

摘要

口腔是一个独特的生态系统,蕴藏着多种微生物,通过平衡的微生物菌群维持健康。破坏平衡可能导致疾病,这就强调了牙龈上皮细胞(GECs)在防止口腔病原微生物危害方面的保护作用。将牙龈上皮细胞的反应从促炎性转变为抗菌性,可能是治疗牙周炎的一种新策略。光生物调节疗法(PBMT)是一种非药物的宿主调节方法,被认为是药物的替代疗法。虽然单一类型的病原体相关分子模式(PAMPs)诱导的宿主细胞反应已被广泛研究,但这一模型并没有解决细胞对完整微生物的反应问题,因为完整微生物会表现出多种可能调节反应的 PAMPs。受此启发,我们开发了一种体外模型,模拟宿主细胞与完整病原体之间的直接相互作用,并从细胞和分子水平评估了 PBMT 对人牙龈角质细胞(HGKs)挑战有活力口腔微生物的反应的影响。我们的数据表明,用特定连续波长(红色:615 纳米;近红外:880 纳米)的 LED 对受到微生物挑战的 HGKs 进行预处理,可诱导产生各种抗菌肽,提高细胞活力和增殖,促进活性氧清除,并降低促炎活性。这些数据还为近红外光处理在增强 HGK 的抗菌活性和减轻细胞炎症方面的功效优于红光提供了可能的解释。综上所述,研究结果表明,PBMT 可增强牙龈上皮的整体屏障功能,同时最大限度地减少炎症介导的底层结构破坏。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Photobiomodulation of Gingival Cells Challenged with Viable Oral Microbes
The oral cavity, a unique ecosystem harboring diverse microorganisms, maintains health through a balanced microflora. Disruption may lead to disease, emphasizing the protective role of gingival epithelial cells (GECs) in preventing harm from pathogenic oral microbes. Shifting GECs’ response from proinflammatory to antimicrobial could be a novel strategy for periodontitis. Photobiomodulation therapy (PBMT), a nonpharmacologic host modulatory approach, is considered an alternative to drugs. While the host cell response induced by a single type of pathogen-associated molecular patterns (PAMPs) was widely studied, this model does not address the cellular response to intact microbes that exhibit multiple PAMPs that might modulate the response. Inspired by this, we developed an in vitro model that simulates direct interactions between host cells and intact pathogens and evaluated the effect of PBMT on the response of human gingival keratinocytes (HGKs) to challenge viable oral microbes at both the cellular and molecular levels. Our data demonstrated that LED pretreatment on microbially challenged HGKs with specific continuous wavelengths (red: 615 nm; near-infrared: 880 nm) induced the production of various antimicrobial peptides, enhanced cell viability and proliferation, promoted reactive oxygen species scavenging, and down-modulated proinflammatory activity. The data also suggest a potential explanation regarding the superior efficacy of near-infrared light treatment compared with red light in enhancing antimicrobial activity and reducing cellular inflammation of HGKs. Taken together, the findings suggest that PBMT enhances the overall barrier function of gingival epithelium while minimizing inflammation-mediated breakdown of the underlying structures.
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来源期刊
ACS Applied Materials & Interfaces
ACS Applied Materials & Interfaces 工程技术-材料科学:综合
CiteScore
16.00
自引率
6.30%
发文量
4978
审稿时长
1.8 months
期刊介绍: ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.
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