根瘤菌对根圈持久性和根定植的决定因素

Hayley E Knights, Vinoy K Ramachandran, Beatriz Jorrin, Raphael Ledermann, Jack D Parsons, Samuel T N Aroney, Philip S Poole
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引用次数: 0

摘要

细菌在根圈中的持久性和根部壁龛的定殖对于许多有益的植物-细菌(包括豆科根瘤菌与其寄主豆科植物之间的相互作用)的建立至关重要。尽管如此,有关豆角根瘤菌的大多数研究都集中在其作为根瘤内共生体的共生生活方式上。在这里,我们使用随机条形码转座子测序(RB-TnSeq)来检测豆角菌在根瘤层中竞争性生长和定殖各种植物物种时的基因贡献。这有助于鉴定 189 个在不同植物根圈中生长所需的常见基因,其中 111 个基因的突变也会影响随后的根定植(根圈渐变),另外 119 个基因是定植所必需的。共同的决定因素揭示了合成必需化合物(氨基酸、核糖核苷酸和辅助因子)、适应新陈代谢功能、对外界刺激做出反应以及承受各种压力(如渗透压变化)的需要。此外,趋化和鞭毛介导的运动是根定植的先决条件。许多基因表现出植物特有的依赖性,突出了对不同植物物种的显著适应性。这项研究加深了人们对促进植物有益细菌的根圈适应性和根定植的因素的了解,有助于开发利用这些细菌为农业造福。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Rhizobium determinants of rhizosphere persistence and root colonisation
Bacterial persistence in the rhizosphere and colonisation of root niches are critical for the establishment of many beneficial plant-bacteria interactions including those between Rhizobium leguminosarum and its host legumes. Despite this, most studies on R. leguminosarum have focused on its symbiotic lifestyle as an endosymbiont in root nodules. Here, we use random barcode transposon sequencing (RB-TnSeq) to assay gene contributions of R. leguminosarum during competitive growth in the rhizosphere and colonisation of various plant species. This facilitated the identification of 189 genes commonly required for growth in diverse plant rhizospheres, mutation of 111 of which also affected subsequent root colonisation (rhizosphere progressive), and a further 119 genes necessary for colonisation. Common determinants reveal a need to synthesise essential compounds (amino acids, ribonucleotides, and cofactors), adapt metabolic function, respond to external stimuli, and withstand various stresses (such as changes in osmolarity). Additionally, chemotaxis and flagella-mediated motility are prerequisites for root colonisation. Many genes showed plant-specific dependencies highlighting significant adaptation to different plant species. This work provides a greater understanding of factors promoting rhizosphere fitness and root colonisation in plant-beneficial bacteria, facilitating their exploitation for agricultural benefit.
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