碳水化合物结合模块:在碳水化合物活性酶的特异性底物结合和性能进化方面的紧凑而有效的配件

IF 12.1 1区 工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yuxian You , Haocun Kong , Caiming Li , Zhengbiao Gu , Xiaofeng Ban , Zhaofeng Li
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引用次数: 0

摘要

碳水化合物结合模块(CBMs)是广泛存在于碳水化合物活性酶(CAZymes)中的独立非催化结构域,它们通过引导附加的催化模块与目标底物结合,在CAZymes的底物结合过程中发挥着至关重要的作用。过去几十年来,CBMs 因其对不同底物的精确识别和选择性亲和力而受到越来越多的研究关注。迄今为止,来自不同产地的 CBMs 已经形成了大量的家族,表现出多种底物类型、结构特征和配体识别机制。此外,通过对 CBMs 特定位点的修饰以及异源 CBMs 与催化结构域的融合,许多 CAZymes 的酶性质和催化模式得到了改善。基于计算生物学、基因编辑和蛋白质工程等前沿技术,CBMs 作为辅助元件已成为 CAZymes 进化和应用的便携式高效工具。为了给未来新型 CBMs 的功能研究、合理设计和定向利用提供理论参考,我们在这篇综述中系统回顾了 CAZyme 衍生 CBMs 的功能相关特性和潜力,包括底物识别和结合机制、对酶性能的非催化贡献、模块修饰以及在各领域的创新应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Carbohydrate binding modules: Compact yet potent accessories in the specific substrate binding and performance evolution of carbohydrate-active enzymes

Carbohydrate binding modules (CBMs) are independent non-catalytic domains widely found in carbohydrate-active enzymes (CAZymes), and they play an essential role in the substrate binding process of CAZymes by guiding the appended catalytic modules to the target substrates. Owing to their precise recognition and selective affinity for different substrates, CBMs have received increasing research attention over the past few decades. To date, CBMs from different origins have formed a large number of families that show a variety of substrate types, structural features, and ligand recognition mechanisms. Moreover, through the modification of specific sites of CBMs and the fusion of heterologous CBMs with catalytic domains, improved enzymatic properties and catalytic patterns of numerous CAZymes have been achieved. Based on cutting-edge technologies in computational biology, gene editing, and protein engineering, CBMs as auxiliary components have become portable and efficient tools for the evolution and application of CAZymes. With the aim to provide a theoretical reference for the functional research, rational design, and targeted utilization of novel CBMs in the future, we systematically reviewed the function-related characteristics and potentials of CAZyme-derived CBMs in this review, including substrate recognition and binding mechanisms, non-catalytic contributions to enzyme performances, module modifications, and innovative applications in various fields.

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来源期刊
Biotechnology advances
Biotechnology advances 工程技术-生物工程与应用微生物
CiteScore
25.50
自引率
2.50%
发文量
167
审稿时长
37 days
期刊介绍: Biotechnology Advances is a comprehensive review journal that covers all aspects of the multidisciplinary field of biotechnology. The journal focuses on biotechnology principles and their applications in various industries, agriculture, medicine, environmental concerns, and regulatory issues. It publishes authoritative articles that highlight current developments and future trends in the field of biotechnology. The journal invites submissions of manuscripts that are relevant and appropriate. It targets a wide audience, including scientists, engineers, students, instructors, researchers, practitioners, managers, governments, and other stakeholders in the field. Additionally, special issues are published based on selected presentations from recent relevant conferences in collaboration with the organizations hosting those conferences.
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