评估未患癌症男性前列腺癌风险升高的 MicroRNA 方法

IF 1.5 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2024-04-01 DOI:10.1615/critreveukaryotgeneexpr.2024053672

Scott Perrapato, Nicholas Farina, Adrian Berg, James Wallace, Steven Ades, Thomas Ahern, Janet Stein, Gary Stein, Jane Lian

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引用次数: 0

摘要

前列腺癌风险评估、治疗决策、治疗评估以及复发的初步迹象都需要客观的标准。循环微RNA被用作生物标志物，用于区分前列腺癌患者与无癌患者或良性前列腺增生患者。研究人员开发了一个由 60 种 microRNA 组成的小组，这些 microRNA 在前列腺癌的发生、发展、转移和复发过程中发挥着既定的作用。利用用于 microRNA 分析的 FirePlex® 平台，我们证明了基于血清的前列腺癌生物标记物快速、高通量测定的有效性和可重复性，该测定避免了对患者标本进行提取和分馏的要求，支持扩大临床研究和诊断应用的可行性。

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A MicroRNA Approach to Evaluate Elevated Prostate Cancer Risk in Cancer-Free Men

Objective criteria are required for prostate cancer risk assessment, treatment decisions, evaluation of therapy, and initial indications of recurrence. Circulating microRNAs were utilized as biomarkers to distinguish prostate cancer patients from cancer-free subjects or those encountering benign prostate hyperplasia. A panel of sixty microRNAs was developed with established roles in prostate cancer initiation, progression, metastasis, and recurrence. Utilizing the FirePlex® platform for microRNA analysis, we demonstrated the efficacy and reproducibility of a rapid, high-throughput, serum-based assay for prostate cancer biomarkers that circumvents the requirement for extraction and fractionation of patient specimens supporting feasibility for expanded clinical research and diagnostic applications.

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来源期刊

Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.