奥克兰岛猪的遗传多样性、生长和心脏功能--器官异种移植的潜在来源

IF 3.3 4区医学 Q2 MEDICINE, RESEARCH & EXPERIMENTAL

Xenotransplantation Pub Date : 2024-04-22 DOI:10.1111/xen.12858

Andreas Lange, Ivica Medugorac, Asghar Ali, Barbara Kessler, Mayuko Kurome, Valeri Zakhartchenko, Sabine E. Hammer, Andreas Hauser, Joachim Denner, Britta Dobenecker, Gerhard Wess, Paul L. J. Tan, Olga Garkavenko, Bruno Reichart, Eckhard Wolf, Elisabeth Kemter

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引用次数: 0

摘要

器官异种移植成功的先决条件之一是猪器官与受体器官的大小要合理匹配。因此，选择合适的源猪遗传背景非常重要。在这项研究中，我们对慕尼黑大学创新医学模型中心（CiMM）建立的奥克兰岛猪群体的身体和器官生长、心脏功能和遗传多样性进行了调查。雌雄奥克兰岛猪肾细胞（经筛选不含猪内源性逆转录病毒 C）从新西兰进口，并通过体细胞核移植（SCNT）建立了创始动物群。从形态上看，奥克兰岛猪与许多国内猪种相比身材较小，因此其器官尺寸非常适合人体移植。此外，对奥克兰岛猪心脏的超声心动图评估表明，在整个研究过程中，各年龄组的心脏结构和功能均正常。单核苷酸多态性（SNP）分析表明，与其他家猪品种相比，奥克兰岛猪的同源性（ROH）更高，并证明编码猪白细胞抗原（SLAs）的整个基因座都是同源的。基于这些发现，奥克兰岛猪是器官异种移植的理想遗传背景。

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Genetic diversity, growth and heart function of Auckland Island pigs, a potential source for organ xenotransplantation

One of the prerequisites for successful organ xenotransplantation is a reasonable size match between the porcine organ and the recipient's organ to be replaced. Therefore, the selection of a suitable genetic background of source pigs is important. In this study, we investigated body and organ growth, cardiac function, and genetic diversity of a colony of Auckland Island pigs established at the Center for Innovative Medical Models (CiMM), LMU Munich. Male and female Auckland Island pig kidney cells (selected to be free of porcine endogenous retrovirus C) were imported from New Zealand, and founder animals were established by somatic cell nuclear transfer (SCNT). Morphologically, Auckland Island pigs have smaller body stature compared to many domestic pig breeds, rendering their organ dimensions well‐suited for human transplantation. Furthermore, echocardiography assessments of Auckland Island pig hearts indicated normal structure and functioning across various age groups throughout the study. Single nucleotide polymorphism (SNP) analysis revealed higher runs of homozygosity (ROH) in Auckland Island pigs compared to other domestic pig breeds and demonstrated that the entire locus coding the swine leukocyte antigens (SLAs) was homozygous. Based on these findings, Auckland Island pigs represent a promising genetic background for organ xenotransplantation.

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来源期刊

Xenotransplantation 医学-医学：研究与实验

CiteScore

6.80

自引率

15.40%

发文量

审稿时长

>12 weeks

期刊介绍： Xenotransplantation provides its readership with rapid communication of new findings in the field of organ and tissue transplantation across species barriers.The journal is not only of interest to those whose primary area is xenotransplantation, but also to veterinarians, microbiologists and geneticists. It also investigates and reports on the controversial theological, ethical, legal and psychological implications of xenotransplantation.