整合药代动力学和网络药理学，解读扶芳沁兰口服液对感染后咳嗽的疗效

IF 3.3 3区医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE

Journal of Traditional and Complementary Medicine Pub Date : 2025-05-01 DOI:10.1016/j.jtcme.2024.04.006

Leyi Huang , Yangyang Wang , Xinyi Yue , Fangfang Yu , Tong Wu , Yimeng Ge , Chunmiao Wang , Meihong Tong

{"title":"整合药代动力学和网络药理学，解读扶芳沁兰口服液对感染后咳嗽的疗效","authors":"Leyi Huang , Yangyang Wang , Xinyi Yue , Fangfang Yu , Tong Wu , Yimeng Ge , Chunmiao Wang , Meihong Tong","doi":"10.1016/j.jtcme.2024.04.006","DOIUrl":null,"url":null,"abstract":"<div><h3>Background and aim</h3><div>Fufang Qinlan oral liquid (QLOL) is a traditional Chinese medicine formula used to treat fevers, sore throats and cough. This study was to evaluate QLOL's therapeutic effects on post infectious cough (PIC) and to investigate Q-markers and action mechanisms by integrating pharmacokinetics and network pharmacology.</div></div><div><h3>Materials and methods</h3><div>PIC rats were measured for cough frequency, organ index, pathological section of lung and inflammatory factors to evaluate the effects of QLOL. Pharmacokinetic analyses were performed using HPLC-QQQ-MS/MS to explore the possible Q-markers of QLOL and the changes of them <em>in vivo</em>. Network pharmacology was used to obtain potential important targets and action mechanisms of treating PIC. The possible hub genes were evaluated using QPCR.</div></div><div><h3>Results</h3><div>The symptoms of cough and lung injury were significantly alleviated and IL-6 and IL-1β were significantly decreased after treatment of QLOL. 6 compounds were considered as possible Q-makers and their pharmacokinetic parameters were analyzed. The compound-target network was constructed to identify 53 important targets. Among them, HSPA8, HSP90AA1, HSP90AB1, YWHAZ, EGFR, ESR1 and EP300 were selected as the core targets because of high degree value and direct connection with inflammation or microbial infection. All 6 compounds had potently binding activity to core targets in molecular docking. The QPCR results showed the up-regulation of core targets expression in QLOL group compared with PIC rats, which validated the effects of QLOL and the accuracy of Q-markers selection.</div></div><div><h3>Conclusion</h3><div>QLOL alleviated PIC symptoms through various targets and mechanisms. The potential Q-markers were baicalein, baicalin, oroxylin A-7-<em>O</em>-glucuronideloside, wogonoside, oroxylin A and forsythoside E.</div></div><div><h3>Taxonomy (classification by evise)</h3><div>the experimental approach.</div></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"15 3","pages":"Pages 250-263"},"PeriodicalIF":3.3000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Integrating pharmacokinetics and network pharmacology to decipher the efficacy of Fufang Qinlan oral liquid on post infectious cough\",\"authors\":\"Leyi Huang , Yangyang Wang , Xinyi Yue , Fangfang Yu , Tong Wu , Yimeng Ge , Chunmiao Wang , Meihong Tong\",\"doi\":\"10.1016/j.jtcme.2024.04.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background and aim</h3><div>Fufang Qinlan oral liquid (QLOL) is a traditional Chinese medicine formula used to treat fevers, sore throats and cough. This study was to evaluate QLOL's therapeutic effects on post infectious cough (PIC) and to investigate Q-markers and action mechanisms by integrating pharmacokinetics and network pharmacology.</div></div><div><h3>Materials and methods</h3><div>PIC rats were measured for cough frequency, organ index, pathological section of lung and inflammatory factors to evaluate the effects of QLOL. Pharmacokinetic analyses were performed using HPLC-QQQ-MS/MS to explore the possible Q-markers of QLOL and the changes of them <em>in vivo</em>. Network pharmacology was used to obtain potential important targets and action mechanisms of treating PIC. The possible hub genes were evaluated using QPCR.</div></div><div><h3>Results</h3><div>The symptoms of cough and lung injury were significantly alleviated and IL-6 and IL-1β were significantly decreased after treatment of QLOL. 6 compounds were considered as possible Q-makers and their pharmacokinetic parameters were analyzed. The compound-target network was constructed to identify 53 important targets. Among them, HSPA8, HSP90AA1, HSP90AB1, YWHAZ, EGFR, ESR1 and EP300 were selected as the core targets because of high degree value and direct connection with inflammation or microbial infection. All 6 compounds had potently binding activity to core targets in molecular docking. The QPCR results showed the up-regulation of core targets expression in QLOL group compared with PIC rats, which validated the effects of QLOL and the accuracy of Q-markers selection.</div></div><div><h3>Conclusion</h3><div>QLOL alleviated PIC symptoms through various targets and mechanisms. The potential Q-markers were baicalein, baicalin, oroxylin A-7-<em>O</em>-glucuronideloside, wogonoside, oroxylin A and forsythoside E.</div></div><div><h3>Taxonomy (classification by evise)</h3><div>the experimental approach.</div></div>\",\"PeriodicalId\":17449,\"journal\":{\"name\":\"Journal of Traditional and Complementary Medicine\",\"volume\":\"15 3\",\"pages\":\"Pages 250-263\"},\"PeriodicalIF\":3.3000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Traditional and Complementary Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2225411024000427\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"INTEGRATIVE & COMPLEMENTARY MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Traditional and Complementary Medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2225411024000427","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"INTEGRATIVE & COMPLEMENTARY MEDICINE","Score":null,"Total":0}

引用次数: 0

摘要

背景与目的复方芩兰口服液（QLOL）是一种治疗发热、喉咙痛、咳嗽的中药方剂。本研究旨在结合药代动力学和网络药理学方法，评价清汤寡糖对感染性咳嗽（PIC）的治疗作用，探讨清汤寡糖的q标记物及其作用机制。材料与方法通过对spic大鼠咳嗽频次、脏器指数、肺病理切片及炎症因子的测定，评价QLOL的作用。采用HPLC-QQQ-MS/MS进行药动学分析，探讨QLOL可能的q -marker及其在体内的变化。利用网络药理学方法获得治疗PIC的潜在重要靶点和作用机制。采用QPCR方法对可能的枢纽基因进行鉴定。结果QLOL治疗后咳嗽、肺损伤症状明显减轻，IL-6、IL-1β水平明显降低。考虑6种可能的q -maker化合物，并分析其药动学参数。构建复合靶点网络，确定53个重要靶点。其中，HSPA8、HSP90AA1、HSP90AB1、YWHAZ、EGFR、ESR1和EP300因其高度值高且与炎症或微生物感染直接相关而被选为核心靶点。在分子对接过程中，6种化合物均与核心靶点具有较强的结合活性。QPCR结果显示，与PIC大鼠相比，QLOL组核心靶点表达上调，验证了QLOL的作用和q标记选择的准确性。结论qlol可通过多种靶点和机制缓解PIC症状。潜在的q标记为黄芩素、黄芩苷、oroxlin A-7- o -葡糖醛酸苷、木犀草苷、oroxlin A和连叶合苷e。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Integrating pharmacokinetics and network pharmacology to decipher the efficacy of Fufang Qinlan oral liquid on post infectious cough

查看原文本刊更多论文

Integrating pharmacokinetics and network pharmacology to decipher the efficacy of Fufang Qinlan oral liquid on post infectious cough

Background and aim

Fufang Qinlan oral liquid (QLOL) is a traditional Chinese medicine formula used to treat fevers, sore throats and cough. This study was to evaluate QLOL's therapeutic effects on post infectious cough (PIC) and to investigate Q-markers and action mechanisms by integrating pharmacokinetics and network pharmacology.

Materials and methods

PIC rats were measured for cough frequency, organ index, pathological section of lung and inflammatory factors to evaluate the effects of QLOL. Pharmacokinetic analyses were performed using HPLC-QQQ-MS/MS to explore the possible Q-markers of QLOL and the changes of them in vivo. Network pharmacology was used to obtain potential important targets and action mechanisms of treating PIC. The possible hub genes were evaluated using QPCR.

Results

The symptoms of cough and lung injury were significantly alleviated and IL-6 and IL-1β were significantly decreased after treatment of QLOL. 6 compounds were considered as possible Q-makers and their pharmacokinetic parameters were analyzed. The compound-target network was constructed to identify 53 important targets. Among them, HSPA8, HSP90AA1, HSP90AB1, YWHAZ, EGFR, ESR1 and EP300 were selected as the core targets because of high degree value and direct connection with inflammation or microbial infection. All 6 compounds had potently binding activity to core targets in molecular docking. The QPCR results showed the up-regulation of core targets expression in QLOL group compared with PIC rats, which validated the effects of QLOL and the accuracy of Q-markers selection.

Conclusion

QLOL alleviated PIC symptoms through various targets and mechanisms. The potential Q-markers were baicalein, baicalin, oroxylin A-7-O-glucuronideloside, wogonoside, oroxylin A and forsythoside E.

Taxonomy (classification by evise)

the experimental approach.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine

CiteScore

9.30

自引率

6.70%

发文量

审稿时长

66 days

期刊介绍： eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.