中国鳉原代肝细胞对雌酮(E1)、17β-雌二醇(E2)和雌三醇(E3)反应的基因表达图谱

IF 5.9 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Yue Wang , Junhui Lv , Zhongtang Xie , Narma Huai , Kailun Zhang , Ying Zhou , Yilihamu Reze , Hua Zhu , Xiqing Li , Zhaobin Zhang
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引用次数: 0

摘要

水生环境中经常检测到含量相对较高的天然雌激素,包括雌酮(E1)、17β-雌二醇(E2)和雌三醇(E3)。最常用的雌激素生物标志物主要在鱼类肝脏中表达。对雌激素处理下的鱼类肝细胞进行全基因谱分析,可提供天然雌激素的精确毒物基因组学信息,而这些信息目前仍鲜为人知。本研究开发了从雄性中华绒鳉(Oryzias sinensis)肝组织中分离和培养原代肝细胞的方法,并利用 RNA-seq 分析了 E1(1、10 和 100 nmol/L)、E2(0.01、0.1、1、10 和 100 nmol/L)和 E3(1、10 和 100 nmol/L)处理下原代肝细胞的全基因表达谱。结果发现,在 E1、E2 和 E3 组中,分别有 175、248 和 218 个基因有差异表达。这些差异表达基因(DEGs)主要富集在基因本体(Gene Ontology,GO)术语 "对雌二醇的反应"、"对雌激素的反应 "和 "脂质转运 "中。此外,slc41a1、zp2.1、esr1、pkd1l1、fam20ca、best3等基因也明显受雌激素的影响而上调,且呈浓度依赖性。利用 RT-qPCR 验证了 RNA-seq 的结果,发现 vtg2 应是研究雌激素的最佳生物标记基因,它能很好地响应天然雌激素和弱雌激素化学物(4-羟基苯甲酸丙酯)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Gene expression profiles of Chinese medaka (Oryzias sinensis) primary hepatocytes in response to estrone (E1), 17β-estradiol (E2) and estriol (E3)

Gene expression profiles of Chinese medaka (Oryzias sinensis) primary hepatocytes in response to estrone (E1), 17β-estradiol (E2) and estriol (E3)

The natural estrogens, including estrone (E1), 17β-estradiol (E2), and estriol (E3), are frequently detected in aquatic environment at relatively high levels. The most commonly used biomarkers for estrogens are mainly expressed in the liver of fish. Analyses of the global gene profiling in fish liver cells under estrogens treatment will provide precise toxicogenomic information of the natural estrogens which is still not well known. In this study, we developed methods for isolation and culture of primary hepatocytes from liver tissue of male Chinese medaka (Oryzias sinensis), and analyzed the global gene expression profiling in the primary hepatocytes treated with E1 (1, 10, and 100 nmol/L), E2 (0.01, 0.1, 1, 10, and 100 nmol/L), and E3 (1, 10, and 100 nmol/L) using RNA-seq. It was found that 175, 248, and 218 genes were differentially expressed in the E1, E2, and E3 groups, respectively. These differentially expressed genes (DEGs) were mainly enriched in Gene Ontology (GO) terms of “response to estradiol”, “response to estrogen”, and “lipid transport”. Of the DEGs, vitellogenin genes, including vtg1, vtg2, and vtg3, were the mostly up-regulated and followed by zona pellucida genes which include zp2.3, zp2l1 and zp3a.2. In addition, genes of slc41a1, zp2.1, esr1, pkd1l1, fam20ca, best3, etc. were also obviously up-regulated by the estrogens in concentration-dependent patterns. RT-qPCR was used to validate the results of RNA-seq and found that vtg2 should be the best biomarker gene for estrogen study, which could well response to natural estrogens and weak estrogenic chemical, propyl 4-hydroxybenzoate.

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来源期刊
Journal of Environmental Sciences-china
Journal of Environmental Sciences-china 环境科学-环境科学
CiteScore
13.70
自引率
0.00%
发文量
6354
审稿时长
2.6 months
期刊介绍: The Journal of Environmental Sciences is an international journal started in 1989. The journal is devoted to publish original, peer-reviewed research papers on main aspects of environmental sciences, such as environmental chemistry, environmental biology, ecology, geosciences and environmental physics. Appropriate subjects include basic and applied research on atmospheric, terrestrial and aquatic environments, pollution control and abatement technology, conservation of natural resources, environmental health and toxicology. Announcements of international environmental science meetings and other recent information are also included.
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