{"title":"中国鳉原代肝细胞对雌酮(E1)、17β-雌二醇(E2)和雌三醇(E3)反应的基因表达图谱","authors":"Yue Wang , Junhui Lv , Zhongtang Xie , Narma Huai , Kailun Zhang , Ying Zhou , Yilihamu Reze , Hua Zhu , Xiqing Li , Zhaobin Zhang","doi":"10.1016/j.jes.2024.03.047","DOIUrl":null,"url":null,"abstract":"<div><p>The natural estrogens, including estrone (E<sub>1</sub>), 17<em>β</em>-estradiol (E<sub>2</sub>), and estriol (E<sub>3</sub>), are frequently detected in aquatic environment at relatively high levels. The most commonly used biomarkers for estrogens are mainly expressed in the liver of fish. Analyses of the global gene profiling in fish liver cells under estrogens treatment will provide precise toxicogenomic information of the natural estrogens which is still not well known. In this study, we developed methods for isolation and culture of primary hepatocytes from liver tissue of male Chinese medaka (<em>Oryzias sinensis</em>), and analyzed the global gene expression profiling in the primary hepatocytes treated with E<sub>1</sub> (1, 10, and 100 nmol/L), E<sub>2</sub> (0.01, 0.1, 1, 10, and 100 nmol/L), and E<sub>3</sub> (1, 10, and 100 nmol/L) using RNA-seq. It was found that 175, 248, and 218 genes were differentially expressed in the E<sub>1</sub>, E<sub>2</sub>, and E<sub>3</sub> groups, respectively. These differentially expressed genes (DEGs) were mainly enriched in Gene Ontology (GO) terms of “response to estradiol”, “response to estrogen”, and “lipid transport”. Of the DEGs, vitellogenin genes, including <em>vtg1, vtg2</em>, and <em>vtg3</em>, were the mostly up-regulated and followed by zona pellucida genes which include <em>zp2.3, zp2l1</em> and <em>zp3a.2</em>. In addition, genes of <em>slc41a1, zp2.1, esr1, pkd1l1, fam20ca, best3</em>, etc. were also obviously up-regulated by the estrogens in concentration-dependent patterns. RT-qPCR was used to validate the results of RNA-seq and found that <em>vtg2</em> should be the best biomarker gene for estrogen study, which could well response to natural estrogens and weak estrogenic chemical, propyl 4-hydroxybenzoate.</p></div>","PeriodicalId":15788,"journal":{"name":"Journal of Environmental Sciences-china","volume":"152 ","pages":"Pages 368-375"},"PeriodicalIF":5.9000,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Gene expression profiles of Chinese medaka (Oryzias sinensis) primary hepatocytes in response to estrone (E1), 17β-estradiol (E2) and estriol (E3)\",\"authors\":\"Yue Wang , Junhui Lv , Zhongtang Xie , Narma Huai , Kailun Zhang , Ying Zhou , Yilihamu Reze , Hua Zhu , Xiqing Li , Zhaobin Zhang\",\"doi\":\"10.1016/j.jes.2024.03.047\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The natural estrogens, including estrone (E<sub>1</sub>), 17<em>β</em>-estradiol (E<sub>2</sub>), and estriol (E<sub>3</sub>), are frequently detected in aquatic environment at relatively high levels. The most commonly used biomarkers for estrogens are mainly expressed in the liver of fish. Analyses of the global gene profiling in fish liver cells under estrogens treatment will provide precise toxicogenomic information of the natural estrogens which is still not well known. In this study, we developed methods for isolation and culture of primary hepatocytes from liver tissue of male Chinese medaka (<em>Oryzias sinensis</em>), and analyzed the global gene expression profiling in the primary hepatocytes treated with E<sub>1</sub> (1, 10, and 100 nmol/L), E<sub>2</sub> (0.01, 0.1, 1, 10, and 100 nmol/L), and E<sub>3</sub> (1, 10, and 100 nmol/L) using RNA-seq. It was found that 175, 248, and 218 genes were differentially expressed in the E<sub>1</sub>, E<sub>2</sub>, and E<sub>3</sub> groups, respectively. These differentially expressed genes (DEGs) were mainly enriched in Gene Ontology (GO) terms of “response to estradiol”, “response to estrogen”, and “lipid transport”. Of the DEGs, vitellogenin genes, including <em>vtg1, vtg2</em>, and <em>vtg3</em>, were the mostly up-regulated and followed by zona pellucida genes which include <em>zp2.3, zp2l1</em> and <em>zp3a.2</em>. In addition, genes of <em>slc41a1, zp2.1, esr1, pkd1l1, fam20ca, best3</em>, etc. were also obviously up-regulated by the estrogens in concentration-dependent patterns. RT-qPCR was used to validate the results of RNA-seq and found that <em>vtg2</em> should be the best biomarker gene for estrogen study, which could well response to natural estrogens and weak estrogenic chemical, propyl 4-hydroxybenzoate.</p></div>\",\"PeriodicalId\":15788,\"journal\":{\"name\":\"Journal of Environmental Sciences-china\",\"volume\":\"152 \",\"pages\":\"Pages 368-375\"},\"PeriodicalIF\":5.9000,\"publicationDate\":\"2024-04-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Environmental Sciences-china\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1001074224001645\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Environmental Sciences-china","FirstCategoryId":"93","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1001074224001645","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Gene expression profiles of Chinese medaka (Oryzias sinensis) primary hepatocytes in response to estrone (E1), 17β-estradiol (E2) and estriol (E3)
The natural estrogens, including estrone (E1), 17β-estradiol (E2), and estriol (E3), are frequently detected in aquatic environment at relatively high levels. The most commonly used biomarkers for estrogens are mainly expressed in the liver of fish. Analyses of the global gene profiling in fish liver cells under estrogens treatment will provide precise toxicogenomic information of the natural estrogens which is still not well known. In this study, we developed methods for isolation and culture of primary hepatocytes from liver tissue of male Chinese medaka (Oryzias sinensis), and analyzed the global gene expression profiling in the primary hepatocytes treated with E1 (1, 10, and 100 nmol/L), E2 (0.01, 0.1, 1, 10, and 100 nmol/L), and E3 (1, 10, and 100 nmol/L) using RNA-seq. It was found that 175, 248, and 218 genes were differentially expressed in the E1, E2, and E3 groups, respectively. These differentially expressed genes (DEGs) were mainly enriched in Gene Ontology (GO) terms of “response to estradiol”, “response to estrogen”, and “lipid transport”. Of the DEGs, vitellogenin genes, including vtg1, vtg2, and vtg3, were the mostly up-regulated and followed by zona pellucida genes which include zp2.3, zp2l1 and zp3a.2. In addition, genes of slc41a1, zp2.1, esr1, pkd1l1, fam20ca, best3, etc. were also obviously up-regulated by the estrogens in concentration-dependent patterns. RT-qPCR was used to validate the results of RNA-seq and found that vtg2 should be the best biomarker gene for estrogen study, which could well response to natural estrogens and weak estrogenic chemical, propyl 4-hydroxybenzoate.
期刊介绍:
The Journal of Environmental Sciences is an international journal started in 1989. The journal is devoted to publish original, peer-reviewed research papers on main aspects of environmental sciences, such as environmental chemistry, environmental biology, ecology, geosciences and environmental physics. Appropriate subjects include basic and applied research on atmospheric, terrestrial and aquatic environments, pollution control and abatement technology, conservation of natural resources, environmental health and toxicology. Announcements of international environmental science meetings and other recent information are also included.