{"title":"从塔萨蚕（antheraea mylitta drury）的各种身体组织中分离出较高分子量的基因组 DNA","authors":"Kumari K, Seraphim E R","doi":"10.36106/ijar/4210207","DOIUrl":null,"url":null,"abstract":"In the present study Genomic DNA was isolated from different body tissue of 3 and 5 instars larva and pupae of Tasar\nsilkworm, Antheraea mylitta Drury. The current article focuses to investigate the various samples for higher molecular\nweight genomic DNA isolation from Tissues commonly used for molecular studies. The modication was targeted to collect the tissue and the\nprimary process of genomic DNA isolation. The cationic detergents used and performance was recorded in gel documentation. This article\ndescribes the best sample to obtain higher molecular weight of genomic DNA from Tasar silkworm.","PeriodicalId":13502,"journal":{"name":"Indian journal of applied research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"ISOLATION OF HIGHER MOLECULAR WEIGHT OF GENOMIC DNA FROM VARIOUS BODY TISSUES OF TASAR SILKWORM, ANTHERAEA MYLITTA DRURY\",\"authors\":\"Kumari K, Seraphim E R\",\"doi\":\"10.36106/ijar/4210207\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"In the present study Genomic DNA was isolated from different body tissue of 3 and 5 instars larva and pupae of Tasar\\nsilkworm, Antheraea mylitta Drury. The current article focuses to investigate the various samples for higher molecular\\nweight genomic DNA isolation from Tissues commonly used for molecular studies. The modication was targeted to collect the tissue and the\\nprimary process of genomic DNA isolation. The cationic detergents used and performance was recorded in gel documentation. This article\\ndescribes the best sample to obtain higher molecular weight of genomic DNA from Tasar silkworm.\",\"PeriodicalId\":13502,\"journal\":{\"name\":\"Indian journal of applied research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2024-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian journal of applied research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.36106/ijar/4210207\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian journal of applied research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.36106/ijar/4210207","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
本研究从 Tasarsilkworm, Antheraea mylitta Drury 的 3 和 5 龄幼虫和蛹的不同身体组织中分离了基因组 DNA。本文重点研究了从分子研究常用组织中分离高分子量基因组 DNA 的各种样本。修改cation的目标是收集组织和基因组 DNA 分离的主要过程。所使用的阳离子洗涤剂及其性能记录在凝胶文件中。本文介绍了从塔沙蚕中获得高分子量基因组DNA的最佳样品。
ISOLATION OF HIGHER MOLECULAR WEIGHT OF GENOMIC DNA FROM VARIOUS BODY TISSUES OF TASAR SILKWORM, ANTHERAEA MYLITTA DRURY
In the present study Genomic DNA was isolated from different body tissue of 3 and 5 instars larva and pupae of Tasar
silkworm, Antheraea mylitta Drury. The current article focuses to investigate the various samples for higher molecular
weight genomic DNA isolation from Tissues commonly used for molecular studies. The modication was targeted to collect the tissue and the
primary process of genomic DNA isolation. The cationic detergents used and performance was recorded in gel documentation. This article
describes the best sample to obtain higher molecular weight of genomic DNA from Tasar silkworm.
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