活体肝移植后 CD4+ T 细胞亚群的变化可预测移植物排斥反应

IF 3.3 Q2 GASTROENTEROLOGY & HEPATOLOGY
Ankur Vagadiya , Rashi Sehgal , Nirupma Trehanpati , Viniyendra Pamecha
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引用次数: 0

摘要

背景和目的调节性 T 细胞(Tregs)在器官移植后的免疫平衡中发挥着关键作用。然而,CD4+ T细胞亚群在早期急性排斥反应中的作用仍不十分清楚。因此,我们的目的是确定活体肝移植(LDLT)中 CD4+ T 细胞亚群的变化。结果 33 例患者接受了随访,其中 11 例(33%)患者出现了早期急性细胞排斥反应(ACR)。在移植周围时间点,Foxp3+ Tregs 的 MFI 比 HC 显著增加(P = 0.04)。然而,CD4+CD25+Foxp3+/CD127- Tregs数量以及分泌IL-10、IL-17和TGF-β的功能性Tregs数量在3个月时比移植前明显减少(P = 0.003)。但与无排斥反应组相比,排斥反应患者的 CD4+CD25+FOXP3+ 和 CD4+CD25+CD127- Tregs 在第 3 天明显减少(P = 0.048)。与无排斥反应组相比,排斥反应患者在移植前后分泌 IL-17 和 TGF-β 的 CD4+CD25+FOXP3+ Tregs 数量也明显减少(P = 0.04,P = 0.03)。此外,排斥反应组在移植前后和第7天显示终末分化效应记忆(TEMRA)减少(P = 0.048 和 P = 0.01)。此外,CD4+中央记忆(CM)在移植周(P = 0.05)、1 个月(P = 0.04)和 3 至 6 个月(P = 0.02)时均有所下降。此外,第 7 天和 1 个月时 CD4+ TEMRA 和 CD4+ CM 的频率降低与排斥反应有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Alterations in CD4+ T-cell Subsets in Living Donor Liver Transplantation Associated With Graft Rejection

Background and objectives

Regulatory T-cells (Tregs) play a key role in immune homeostasis after organ transplantation. However, the role of CD4+ T cell subsets in early acute rejection is still not well understood. Therefore, our aim was to determine changes in CD4+ T-cell subsets in living donor liver transplantation (LDLT).

Methods

LDLT patients were assessed for T-cell subsets, Tregs frequencies and their functionality by flow-cytometry at peri- and post-transplant in the span of 1 year.

Results

33 patients were followed up and 11 (33%) patients have developed early acute cellular rejection (ACR). At peri-transplant time point, MFI of Foxp3+ Tregs was significantly increased compared to HC (P = 0.04). However, CD4+CD25+Foxp3+/CD127 Tregs numbers and IL-10, IL-17 and TGF-β secreting functional Tregs were significantly decreased at 3 months compared to peri-transplant (P = 0.003). But in patients with rejection, CD4+CD25+FOXP3+ and CD4+CD25+CD127 Tregs were significantly decreased at day 3 compared to no rejection group (P = 0.048). Patients with rejection also showed significantly decreased numbers of IL-17 and TGF-β secreting CD4+CD25+FOXP3+ Tregs at peri-transplant time (P = 0.04, P = 0.03) compared to no rejection. Further, rejection group showed decreased terminally differentiated effector memory (TEMRA) at peri-transplant and day 7 (P = 0.048 and P = 0.01). Additionally, CD4+ central memory (CM) was decreased at peri-transplant (P = 0.05), 1 month (P = 0.04), and 3 to 6 month (P = 0.02).

Interpretation and conclusion

Tregs frequencies were significantly decreased in peri-TX in rejection patients. Further, decreased frequencies of CD4+ TEMRA and CD4+ CM at day 7 and 1 month were associated with rejection.

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来源期刊
Journal of Clinical and Experimental Hepatology
Journal of Clinical and Experimental Hepatology GASTROENTEROLOGY & HEPATOLOGY-
CiteScore
4.90
自引率
16.70%
发文量
537
审稿时长
64 days
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