Chee-Seng Lee , Chia-Ray Lin , Huey-Huey Chua , Jia-Feng Wu , Kai-Chi Chang , Yen-Hsuan Ni , Mei-Hwei Chang , Huey-Ling Chen
{"title":"肠道长双歧杆菌与胆道闭锁患者较好的原肝存活率有关","authors":"Chee-Seng Lee , Chia-Ray Lin , Huey-Huey Chua , Jia-Feng Wu , Kai-Chi Chang , Yen-Hsuan Ni , Mei-Hwei Chang , Huey-Ling Chen","doi":"10.1016/j.jhepr.2024.101090","DOIUrl":null,"url":null,"abstract":"<div><h3>Background & Aims</h3><p>The gut microbiome plays an important role in liver diseases, but its specific impact on biliary atresia (BA) remains to be explored. We aimed to investigate the microbial signature in the early life of patients with BA and to analyze its influence on long-term outcomes.</p></div><div><h3>Methods</h3><p>Fecal samples (n = 42) were collected from infants with BA before and after Kasai portoenterostomy (KPE). The stool microbiota was analyzed using 16S rRNA next-generation sequencing and compared with that of age-matched healthy controls (HCs). Shotgun metagenomic sequencing analysis was employed to confirm the bacterial composition in 10 fecal samples before KPE. The correlation of the microbiome signature with liver function and long-term outcomes was assessed.</p></div><div><h3>Results</h3><p>In the 16S rRNA next-generation sequencing analysis of fecal microbiota, the alpha and beta diversity analyses revealed significant differences between HCs and patients with BA before and after KPE. The difference in microbial composition analyzed by linear discriminant analysis and random forest classification revealed that the abundance of <em>Bifidobacterium longum</em> (<em>B. longum</em>) was significantly lower in patients before and after KPE than in HCs. The abundance of <em>B. longum</em> was negatively correlated with the gamma-glutamyltransferase level after KPE (<em>p <</em>0.05). Patients with early detectable <em>B. longum</em> had significantly lower total and direct bilirubin 3 months after KPE (<em>p <</em>0.005) and had a significantly lower liver transplantation rate (hazard ratio: 0.16, 95% CI 0.03-0.83, <em>p =</em> 0.029). Shotgun metagenomic sequencing also revealed that patients with BA and detectable <em>B. longum</em> had reduced total and direct bilirubin after KPE.</p></div><div><h3>Conclusion</h3><p>The gut microbiome of patients with BA differed from that of HCs, with a notable abundance of <em>B. longum</em> in early infancy correlating with better long-term outcomes.</p></div><div><h3>Impact and implications</h3><p><em>Bifidobacterium longum</em> (<em>B. longum</em>) is a beneficial bacterium commonly found in the human gut. It has been studied for its potential impacts on various health conditions. In patients with biliary atresia, we found that a greater abundance of <em>B. longum</em> in the fecal microbiome is associated with improved clinical outcomes. This suggests that early colonization and increasing <em>B. longum</em> levels in the gut could be a therapeutic strategy to improve the prognosis of patients with biliary atresia.</p></div>","PeriodicalId":14764,"journal":{"name":"JHEP Reports","volume":null,"pages":null},"PeriodicalIF":9.5000,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2589555924000946/pdfft?md5=d79304e95bb4211ee9327dafd74559e5&pid=1-s2.0-S2589555924000946-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Gut Bifidobacterium longum is associated with better native liver survival in patients with biliary atresia\",\"authors\":\"Chee-Seng Lee , Chia-Ray Lin , Huey-Huey Chua , Jia-Feng Wu , Kai-Chi Chang , Yen-Hsuan Ni , Mei-Hwei Chang , Huey-Ling Chen\",\"doi\":\"10.1016/j.jhepr.2024.101090\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background & Aims</h3><p>The gut microbiome plays an important role in liver diseases, but its specific impact on biliary atresia (BA) remains to be explored. We aimed to investigate the microbial signature in the early life of patients with BA and to analyze its influence on long-term outcomes.</p></div><div><h3>Methods</h3><p>Fecal samples (n = 42) were collected from infants with BA before and after Kasai portoenterostomy (KPE). The stool microbiota was analyzed using 16S rRNA next-generation sequencing and compared with that of age-matched healthy controls (HCs). Shotgun metagenomic sequencing analysis was employed to confirm the bacterial composition in 10 fecal samples before KPE. The correlation of the microbiome signature with liver function and long-term outcomes was assessed.</p></div><div><h3>Results</h3><p>In the 16S rRNA next-generation sequencing analysis of fecal microbiota, the alpha and beta diversity analyses revealed significant differences between HCs and patients with BA before and after KPE. The difference in microbial composition analyzed by linear discriminant analysis and random forest classification revealed that the abundance of <em>Bifidobacterium longum</em> (<em>B. longum</em>) was significantly lower in patients before and after KPE than in HCs. The abundance of <em>B. longum</em> was negatively correlated with the gamma-glutamyltransferase level after KPE (<em>p <</em>0.05). Patients with early detectable <em>B. longum</em> had significantly lower total and direct bilirubin 3 months after KPE (<em>p <</em>0.005) and had a significantly lower liver transplantation rate (hazard ratio: 0.16, 95% CI 0.03-0.83, <em>p =</em> 0.029). Shotgun metagenomic sequencing also revealed that patients with BA and detectable <em>B. longum</em> had reduced total and direct bilirubin after KPE.</p></div><div><h3>Conclusion</h3><p>The gut microbiome of patients with BA differed from that of HCs, with a notable abundance of <em>B. longum</em> in early infancy correlating with better long-term outcomes.</p></div><div><h3>Impact and implications</h3><p><em>Bifidobacterium longum</em> (<em>B. longum</em>) is a beneficial bacterium commonly found in the human gut. It has been studied for its potential impacts on various health conditions. In patients with biliary atresia, we found that a greater abundance of <em>B. longum</em> in the fecal microbiome is associated with improved clinical outcomes. This suggests that early colonization and increasing <em>B. longum</em> levels in the gut could be a therapeutic strategy to improve the prognosis of patients with biliary atresia.</p></div>\",\"PeriodicalId\":14764,\"journal\":{\"name\":\"JHEP Reports\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":9.5000,\"publicationDate\":\"2024-04-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2589555924000946/pdfft?md5=d79304e95bb4211ee9327dafd74559e5&pid=1-s2.0-S2589555924000946-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"JHEP Reports\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2589555924000946\",\"RegionNum\":1,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"GASTROENTEROLOGY & HEPATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"JHEP Reports","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2589555924000946","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}
Gut Bifidobacterium longum is associated with better native liver survival in patients with biliary atresia
Background & Aims
The gut microbiome plays an important role in liver diseases, but its specific impact on biliary atresia (BA) remains to be explored. We aimed to investigate the microbial signature in the early life of patients with BA and to analyze its influence on long-term outcomes.
Methods
Fecal samples (n = 42) were collected from infants with BA before and after Kasai portoenterostomy (KPE). The stool microbiota was analyzed using 16S rRNA next-generation sequencing and compared with that of age-matched healthy controls (HCs). Shotgun metagenomic sequencing analysis was employed to confirm the bacterial composition in 10 fecal samples before KPE. The correlation of the microbiome signature with liver function and long-term outcomes was assessed.
Results
In the 16S rRNA next-generation sequencing analysis of fecal microbiota, the alpha and beta diversity analyses revealed significant differences between HCs and patients with BA before and after KPE. The difference in microbial composition analyzed by linear discriminant analysis and random forest classification revealed that the abundance of Bifidobacterium longum (B. longum) was significantly lower in patients before and after KPE than in HCs. The abundance of B. longum was negatively correlated with the gamma-glutamyltransferase level after KPE (p <0.05). Patients with early detectable B. longum had significantly lower total and direct bilirubin 3 months after KPE (p <0.005) and had a significantly lower liver transplantation rate (hazard ratio: 0.16, 95% CI 0.03-0.83, p = 0.029). Shotgun metagenomic sequencing also revealed that patients with BA and detectable B. longum had reduced total and direct bilirubin after KPE.
Conclusion
The gut microbiome of patients with BA differed from that of HCs, with a notable abundance of B. longum in early infancy correlating with better long-term outcomes.
Impact and implications
Bifidobacterium longum (B. longum) is a beneficial bacterium commonly found in the human gut. It has been studied for its potential impacts on various health conditions. In patients with biliary atresia, we found that a greater abundance of B. longum in the fecal microbiome is associated with improved clinical outcomes. This suggests that early colonization and increasing B. longum levels in the gut could be a therapeutic strategy to improve the prognosis of patients with biliary atresia.
期刊介绍：
JHEP Reports is an open access journal that is affiliated with the European Association for the Study of the Liver (EASL). It serves as a companion journal to the highly respected Journal of Hepatology.
The primary objective of JHEP Reports is to publish original papers and reviews that contribute to the advancement of knowledge in the field of liver diseases. The journal covers a wide range of topics, including basic, translational, and clinical research. It also focuses on global issues in hepatology, with particular emphasis on areas such as clinical trials, novel diagnostics, precision medicine and therapeutics, cancer research, cellular and molecular studies, artificial intelligence, microbiome research, epidemiology, and cutting-edge technologies.
In summary, JHEP Reports is dedicated to promoting scientific discoveries and innovations in liver diseases through the publication of high-quality research papers and reviews covering various aspects of hepatology.
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