在新西兰奥特亚罗瓦,仅使用 CHROMagar™ STEC 培养基并不能回收所有临床相关的产志贺毒素大肠埃希菌。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Lucia Rivas, David Duncan, Jing Wang, Hilary Miller, J. Wright
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引用次数: 0

摘要

新西兰奥特亚罗瓦的诊断实验室将志贺毒素基因阳性的粪便样本培养物转给国家肠道参考实验室,以分离出产志贺毒素的大肠埃希氏菌(STEC),进行流行病学分型。由于转介的培养基存在差异,因此使用了代表 28 种不同血清型的 75 例 STEC 临床分离样本来评估六种市售培养基,并为临床实验室提供指导。随后对建议进行了为期三个月的测试,通过全基因组测序分析对照所推荐的培养基对 STEC 分离和确证情况进行评估。经证实,CHROMagar™ STEC(CHR-STEC;法国巴黎 CHROMagar 微生物公司)或 CH-STEC 加头孢克肟-碲化山梨醇麦康凯琼脂(CT-SMAC)的效果不如 CH-STEC 加血琼脂加万古霉素、头孢磺啶和头孢克肟 (BVCC)。与 CH-STEC 和 BVCC 的组合(42 个)相比,前者确认的 STEC 类型较少(18 个)。STEC 在 CH-STEC 上的生长能力与 ter 基因簇和 eae 的存在有明显的关联(P <0.05)。在 CH-STEC 和 BVCC 上培养筛选出的 STEC 阳性样本,可确保从新西兰所有临床样本中回收的 STEC 始终高于仅在 CH-STEC 上培养的样本。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Using CHROMagar™ STEC medium exclusively does not recover all clinically relevant Shiga-toxin producing Escherichia coli in Aotearoa, New Zealand.
Diagnostic laboratories in Aotearoa New Zealand refer cultures from faecal samples positive for Shiga-toxin genes to the national Enteric Reference Laboratory for isolation of Shiga-toxin producing Escherichia coli (STEC) for epidemiological typing. As there was variation in the culture media being referred, a panel of 75 clinical isolates of STEC, representing 28 different serotypes, was used to assess six commercially available media and provide guidance to clinical laboratories. Recommendations were subsequently tested for a three-month period where STEC isolations and confirmations were assessed by whole genome sequencing analysis against the culture media referred. CHROMagar™ STEC (CH-STEC; CHROMagar Microbiology, Paris, France) or CH-STEC plus cefixime-tellurite sorbitol MacConkey agar (CT-SMAC) was confirmed inferior to CH-STEC plus Blood agar with vancomycin, cefsulodin and cefixime (BVCC). The former resulted in fewer STEC types (n = 18) being confirmed compared to those from a combination of CH-STEC and BVCC (n = 42). A significant (P <0.05) association with an STEC's ability to grow on CH-STEC and the presence of the ter gene cluster, and eae was observed. Culturing screen positive STEC samples onto both CH-STEC and BVCC ensures a consistently higher recovery of STEC from all clinical samples in NZ than CH-STEC alone.
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
期刊介绍: ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.
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