高频听力损伤与 Hensen 细胞的不同支持潜能有关:SMART-Seq2 RNA测序。

IF 5.7 4区 生物学 Q1 BIOLOGY
Yiding Yu, Yueping Li, C. Wen, Fengbo Yang, Xuemin Chen, Wenqi Yi, Lin Deng, Xiaohua Cheng, Ning Yu, Lihui Huang
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引用次数: 0

摘要

听力损失是影响沟通、健康和医疗成本的第三大身体疾病。感音神经性听力损失通常首先发生在高频区域(基底转向),然后向低频区域(顶端转向)发展。然而,其机制仍不清楚。支持细胞在维持耳蜗正常功能方面起着至关重要的作用。不同频率区域的支持细胞的功能和支持能力可能不同。Hensen's 细胞是独特的支持细胞类型之一,其特点是胞质中有脂滴(LDs)。在这里,我们研究了 Hensen's 细胞沿耳蜗轴线的形态和基因表达差异。我们观察到 Hensen's 细胞的形态特征沿耳蜗声调轴呈梯度变化,在顶端 Hensen's 细胞中观察到更大更丰富的 LDs。Smart-seq2 RNA-seq发现了顶端和基底Hensen's细胞之间的差异表达基因(DEGs),这些基因聚集在几个通路中,包括不饱和脂肪酸生物合成、胆固醇代谢和脂肪酸分解,这些通路与不同的能量储存能力和代谢潜力有关。这些发现表明,顶端和基底亨森氏细胞在脂质代谢和氧化供能方面可能存在差异,这与亨森氏细胞的形态差异是一致的。我们还发现了与遗传性听力损失(HHL)、噪声诱导性听力损失(NIHL)和年龄相关性听力损失(ARHL)有关的候选基因的不同表达模式。这些发现表明耳蜗轴上的SC具有功能异质性,有助于我们了解耳蜗生理学,并为未来的听力损失研究提供分子基础证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
High-frequency hearing vulnerability associated with the different supporting potential of Hensen's cells: SMART-Seq2 RNA sequencing.
Hearing loss is the third most prevalent physical condition affecting communication, well-being, and healthcare costs. Sensorineural hearing loss often occurs first in the high-frequency region (basal turn), then towards the low-frequency region (apical turn). However, the mechanism is still unclear. Supporting cells play a critical role in the maintenance of normal cochlear function. The function and supporting capacity of these cells may be different from different frequency regions. Hensen's cells are one of the unique supporting cell types characterized by lipid droplets (LDs) in the cytoplasm. Here, we investigated the morphological and gene expression differences of Hensen's cells along the cochlear axis. We observed a gradient change in the morphological characteristics of Hensen's cells along the cochlear tonotopic axis, with larger and more abundant LDs observed in apical Hensen's cells. Smart-seq2 RNA-seq revealed differentially expressed genes (DEGs) between apical and basal Hensen's cells that clustered in several pathways, including unsaturated fatty acid biosynthesis, cholesterol metabolism, and fatty acid catabolism, which are associated with different energy storage capacities and metabolic potential. These findings suggest potential differences in lipid metabolism and oxidative energy supply between apical and basal Hensen's cells, which is consistent with the morphological differences of Hensen's cells. We also found differential expression patterns of candidate genes associated with hereditary hearing loss (HHL), noise-induced hearing loss (NIHL), and age-related hearing loss (ARHL). These findings indicate functional heterogeneity of SCs along the cochlear axis, contribute to our understanding of cochlear physiology and provide molecular basis evidence for future studies of hearing loss.
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来源期刊
CiteScore
13.60
自引率
1.80%
发文量
47
审稿时长
>12 weeks
期刊介绍: BioScience Trends (Print ISSN 1881-7815, Online ISSN 1881-7823) is an international peer-reviewed journal. BioScience Trends devotes to publishing the latest and most exciting advances in scientific research. Articles cover fields of life science such as biochemistry, molecular biology, clinical research, public health, medical care system, and social science in order to encourage cooperation and exchange among scientists and clinical researchers.
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