I. Chóez-Guaranda, Michael Rendon, Stalin Peralta, Andrea Villegas, Patricia Manzano
{"title":"利用毛细管电泳和二极管阵列检测法同时测定商业植物生物刺激剂中脱落酸、3-吲哚乙酸和赤霉素的有效方法","authors":"I. Chóez-Guaranda, Michael Rendon, Stalin Peralta, Andrea Villegas, Patricia Manzano","doi":"10.17268/sci.agropecu.2024.014","DOIUrl":null,"url":null,"abstract":"Phytohormones, also known as plant hormones, are naturally occurring chemical compounds that regulate various physiological processes in plants. In this work, a capillary electrophoretic coupled to a diode array detector (CE-DAD) method was developed and validated for the simultaneous quantification of abscisic acid (ABA), 3-indolacetic acid (IAA), and gibberellic acid (GA3) in commercial plant biostimulants. Sample preparation was carried out by liquid-liquid extraction using ethyl acetate. CE separation was performed in a fused-silica capillary and background electrolyte (BGE) consisting of borate buffer (50 mM, pH 9.3) applying a high voltage of 20 kV, a pressure of 50 mbar, and injection time of 35 s. The ABA, IAA, and GA3 were detected at 254, 220 and 195 nm respectively. The CE-DAD method validation results showed acceptable specificity, linearity, accuracy, and precision in the concentration range of 10-100 µg/mL for all compounds according to the (International Conference Harmonisation) ICH guidelines. The proposed method was satisfactory applied to the analysis of cited plant hormones in biostimulants and suggest that sample preparation is a reliable step for extraction of phytohormones containing carboxyl groups. Therefore, the developed and validated method could be implemented as a low-cost and fast analytical tool for quality control purposes.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":"175 1","pages":""},"PeriodicalIF":16.4000,"publicationDate":"2024-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Effective method for simultaneous determination of abscisic acid, 3-indolacetic acid and gibberellic acid in commercial plant biostimulants by capillary electrophoresis with diode array detection\",\"authors\":\"I. Chóez-Guaranda, Michael Rendon, Stalin Peralta, Andrea Villegas, Patricia Manzano\",\"doi\":\"10.17268/sci.agropecu.2024.014\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Phytohormones, also known as plant hormones, are naturally occurring chemical compounds that regulate various physiological processes in plants. In this work, a capillary electrophoretic coupled to a diode array detector (CE-DAD) method was developed and validated for the simultaneous quantification of abscisic acid (ABA), 3-indolacetic acid (IAA), and gibberellic acid (GA3) in commercial plant biostimulants. Sample preparation was carried out by liquid-liquid extraction using ethyl acetate. CE separation was performed in a fused-silica capillary and background electrolyte (BGE) consisting of borate buffer (50 mM, pH 9.3) applying a high voltage of 20 kV, a pressure of 50 mbar, and injection time of 35 s. The ABA, IAA, and GA3 were detected at 254, 220 and 195 nm respectively. The CE-DAD method validation results showed acceptable specificity, linearity, accuracy, and precision in the concentration range of 10-100 µg/mL for all compounds according to the (International Conference Harmonisation) ICH guidelines. The proposed method was satisfactory applied to the analysis of cited plant hormones in biostimulants and suggest that sample preparation is a reliable step for extraction of phytohormones containing carboxyl groups. Therefore, the developed and validated method could be implemented as a low-cost and fast analytical tool for quality control purposes.\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":\"175 1\",\"pages\":\"\"},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2024-04-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.17268/sci.agropecu.2024.014\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17268/sci.agropecu.2024.014","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Effective method for simultaneous determination of abscisic acid, 3-indolacetic acid and gibberellic acid in commercial plant biostimulants by capillary electrophoresis with diode array detection
Phytohormones, also known as plant hormones, are naturally occurring chemical compounds that regulate various physiological processes in plants. In this work, a capillary electrophoretic coupled to a diode array detector (CE-DAD) method was developed and validated for the simultaneous quantification of abscisic acid (ABA), 3-indolacetic acid (IAA), and gibberellic acid (GA3) in commercial plant biostimulants. Sample preparation was carried out by liquid-liquid extraction using ethyl acetate. CE separation was performed in a fused-silica capillary and background electrolyte (BGE) consisting of borate buffer (50 mM, pH 9.3) applying a high voltage of 20 kV, a pressure of 50 mbar, and injection time of 35 s. The ABA, IAA, and GA3 were detected at 254, 220 and 195 nm respectively. The CE-DAD method validation results showed acceptable specificity, linearity, accuracy, and precision in the concentration range of 10-100 µg/mL for all compounds according to the (International Conference Harmonisation) ICH guidelines. The proposed method was satisfactory applied to the analysis of cited plant hormones in biostimulants and suggest that sample preparation is a reliable step for extraction of phytohormones containing carboxyl groups. Therefore, the developed and validated method could be implemented as a low-cost and fast analytical tool for quality control purposes.
期刊介绍:
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.