蛋白质 L 非受力区的突变影响了受力折叠行为

IF 1.5 4区物理与天体物理 Q2 PHYSICS, MULTIDISCIPLINARY

Chinese Physics B Pub Date : 2024-04-12 DOI:10.1088/1674-1056/ad3dcd

Huanjie Jiang, Yanwei Wang, Jiayuan Chen, Dan Hu, Hai Pan, Zilong Guo, Hu Chen

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引用次数: 0

摘要

单分子磁镊（MT）揭示了几种双态蛋白质（如 GB1 和 Csp）在展开路径上的多重转换障碍。在这项研究中，我们利用 MT 测定了蛋白质 L（PLWT）及其 Y47W 突变体（PLY47W）随力折叠和展开的速率。测量在 3 至 120 pN 的力范围内进行。值得注意的是，PLWT 和 PWY47W 的展开率在低于 50 pN 和高于 60 pN 时表现出不同的力敏感性，这意味着存在双屏障自由能景观。PLWT 和 PLY47W 都具有相同的随力折叠率和相同的转换壁垒，但 PLY47W 的解折率比 PLWT 快。我们的发现表明，受力区域外的残基也会影响力诱导的解折动力学。

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Mutation in a Non-Force-Bearing Region of Protein L Influences Force-Dependent Unfolding Behavior

Single-molecule magnetic tweezers (MT) have revealed multiple transition barriers along the unfolding pathway of several two-state proteins, such as GB1 and Csp. In this study, we utilized MT to measure the force-dependent folding and unfolding rates of both the Protein L (PLWT) and its Y47W mutant (PLY47W) where the mutation point is not at the force-bearing β-strands. The measurements were conducted within a force range of 3 to 120 pN. Notably, the unfolding rates of both PLWT and PWY47W exhibit distinct force sensitivities below 50 pN and above 60 pN, implying a two-barrier free energy landscape. Both PLWT and PLY47W share the same force-dependent folding rate and the same transition barriers, but the unfolding rate of PLY47W is faster than PLWT. Our finding demonstrates that the residue outside of the force-bearing region will also affect the force-induced unfolding dynamics.

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来源期刊

Chinese Physics B 物理-物理：综合

CiteScore

2.80

自引率

23.50%

发文量

15667

审稿时长

2.4 months

期刊介绍： Chinese Physics B is an international journal covering the latest developments and achievements in all branches of physics worldwide (with the exception of nuclear physics and physics of elementary particles and fields, which is covered by Chinese Physics C). It publishes original research papers and rapid communications reflecting creative and innovative achievements across the field of physics, as well as review articles covering important accomplishments in the frontiers of physics. Subject coverage includes: Condensed matter physics and the physics of materials Atomic, molecular and optical physics Statistical, nonlinear and soft matter physics Plasma physics Interdisciplinary physics.