结直肠腺癌微卫星不稳定性与 BRAF 和 p53 基因突变及组织病理学参数的关系

IF 0.9 4区 医学 Q3 SURGERY
Özgecan Gündoğar, S. Bektaş, Emine Yıldırım, Doğan Gönüllü
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引用次数: 0

摘要

目的:本研究旨在阐明微卫星不稳定性(MSI)状态、BRAF突变和p53反应与结直肠癌病理参数和生存结果之间的关系。 材料与方法:对130份腺癌样本(其中65份来自右侧结肠,65份来自左侧结肠)进行了MutL同源1(MLH1)、减数分裂后分离增加2(PMS2)、MutS同源2(MSH2)、MutS同源6(MSH6)、BRAF和p53抗体检测。统计分析了 MSI 状态与 BRAF 突变、p53 反应、临床和病理参数以及存活时间的关系。 结果发现MSI与右侧结肠定位、肿瘤大小、组织学分级、肿瘤上皮内浸润淋巴细胞、克罗恩样淋巴细胞反应、膨胀性生长模式和BRAF突变之间存在统计学意义上的显著关系(P < 0.05)。MSI状态与无病生存期或总生存期之间没有明显相关性(P > 0.05)。 结论在结直肠腺癌中,MSI和BRAF突变与表明宿主免疫反应和预后组织病理学参数(包括肿瘤大小和组织学分级)的参数相关。对 MSI 状态和 BRAF 突变的评估尤其有助于预测分化较差的结直肠腺癌的预后并指导治疗管理。了解结直肠癌的分子致癌机制并根据分子基础制定治疗算法将提高治疗的成功率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Relationship of Microsatellite Instability with BRAF and p53 Mutations and Histopathological Parameters in Colorectal Adenocarcinoma
Aim: This study aims to elucidate the associations between microsatellite instability (MSI) status, BRAF mutation, and p53 reactions with pathological parameters and survival outcomes in colorectal carcinoma.  Material and Method: MutL homologous 1 (MLH1), Postmeiotic segregation increased 2 (PMS2), MutS homologous 2 (MSH2), MutS homologous 6 (MSH6), BRAF, and p53 antibodies were performed on 130 adenocarcinoma samples, including 65 from the right colon and 65 from the left colon. The relationships of MSI status with BRAF mutation, p53 reaction, clinical and pathological parameters, and survival times were statistically analyzed.  Results: A statistically significant relationship was found between MSI and right colon localization, tumor size, histological grade, intraepithelial tumor-infiltrating lymphocytes, Crohn-like lymphocytic reaction, expansive growth pattern, and BRAF mutation (p < 0.05). No significant correlation was found between MSI status and the disease-free or overall survival times (p > 0.05).  Conclusion: In colorectal adenocarcinoma, MSI and BRAF mutation are associated with parameters, indicating the host immune response and prognostic histopathological parameters, including tumor size and histological grade. The evaluation of MSI status and BRAF mutation can be particularly informative for predicting the prognosis and guiding the treatment management in poorly differentiated colorectal adenocarcinoma. Understanding the mechanisms of molecular carcinogenesis in colorectal carcinoma and organizing treatment algorithms based on molecular foundations will increase the success of the treatment.
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来源期刊
CiteScore
0.90
自引率
12.50%
发文量
116
审稿时长
>12 weeks
期刊介绍: Annali Italiani di Chirurgia is a bimonthly journal and covers all aspects of surgery:elective, emergency and experimental surgery, as well as problems involving technology, teaching, organization and forensic medicine. The articles are published in Italian or English, though English is preferred because it facilitates the international diffusion of the journal (v.Guidelines for Authors and Norme per gli Autori). The articles published are divided into three main sections:editorials, original articles, and case reports and innovations.
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