揭示早期人类唾液污染对引导骨再生膜的影响。

IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Marcel F. Kunrath, Paula Milena Giraldo-Osorno, Karina Mendes, Ana T. P. C. Gomes, Nuno Rosa, Marlene Barros, Christer Dahlin
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引用次数: 0

摘要

目的 GBR 膜具有各种表面特性,可在再生临床程序中引起积极反应;牙科临床医生试图采用各种技术防止受污染的口腔液体与这些生物材料直接相互作用。然而,在应用 GBR 膜的口腔外科手术中,唾液会不间断地流出,这表明唾液会与生物材料和周围的口腔组织发生持续的相互作用。这项基础研究旨在调查用于引导骨再生(GBR)的膜的物理、化学和关键生物特性可能因与人类唾液的早期分离相互作用而发生的改变。随后,对膜的理化性质、蛋白质定量、DNA 和 16S rRNA 水平、两种不同细胞系 1 天和 7 天的存活率以及 ALP 活性进行了评估。结果发现了定量的形态学变化;DNA 提取和 16S 定量显示了显著较高的数值。此外,唾液与可降解膜相互作用后,HGF-1 和 MC3T3-E1 细胞的活力明显降低(p < .05)。这些结果表明,生物降解膜对隔离的早期人类唾液相互作用具有易感性,表明其结构形态受损,HGF-1 和 MC3T3-E1 的存活率降低,DNA/16S rRNA 的吸收/粘附率升高。因此,临床口腔程序可能需要相应的改进。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Unveiling the consequences of early human saliva contamination on membranes for guided bone regeneration

Unveiling the consequences of early human saliva contamination on membranes for guided bone regeneration

Aims

GBR membranes have various surface properties designed to elicit positive responses in regenerative clinical procedures; dental clinicians attempt to employ techniques to prevent the direct interaction of contaminated oral fluids with these biomaterials. However, saliva is uninterruptedly exhibited in oral surgical procedures applying GBR membranes, suggesting a persistent interaction with biomaterials and the surrounding oral tissues. This fundamental study aimed to investigate potential alterations in the physical, chemical, and key biological properties of membranes for guided bone regeneration (GBR) caused by isolated early interaction with human saliva.

Methods

A reproducible step-by-step protocol for collecting and interacting human saliva with membranes was developed. Subsequently, membranes were evaluated for their physicochemical properties, protein quantification, DNA, and 16S rRNA levels viability of two different cell lines at 1 and 7 days, and ALP activity. Non-interacted membranes and pure saliva of donors were applied as controls.

Results

Qualitative morphological alterations were noticed; DNA extraction and 16S quantification revealed significantly higher values. Furthermore, the viability of HGF-1 and MC3T3-E1 cells was significantly (p < .05) reduced following saliva interaction with biodegradable membranes. Saliva contamination did not prejudice PTFE membranes significantly in any biological assay.

Conclusions

These outcomes demonstrated a susceptible response of biodegradable membranes to isolated early human saliva interaction, suggesting impairment of structural morphology, reduced viability to HGF-1 and MC3T3-E1, and higher absorption/adherence of DNA/16S rRNA. As a result, clinical oral procedures may need corresponding refinements.

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来源期刊
Journal of periodontal research
Journal of periodontal research 医学-牙科与口腔外科
CiteScore
6.90
自引率
5.70%
发文量
103
审稿时长
6-12 weeks
期刊介绍: The Journal of Periodontal Research is an international research periodical the purpose of which is to publish original clinical and basic investigations and review articles concerned with every aspect of periodontology and related sciences. Brief communications (1-3 journal pages) are also accepted and a special effort is made to ensure their rapid publication. Reports of scientific meetings in periodontology and related fields are also published. One volume of six issues is published annually.
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