通过傅立叶变换红外光谱法、MALDI 质谱法和全基因组测序对细菌进行鉴别。

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2024-04-23 DOI:10.2217/fmb-2024-0043

Rachel McGalliard, H. Muhamadali, N. AlMasoud, S. Haldenby, Valeria Romero-Soriano, Ellie Allman, Yun Xu, Adam P Roberts, Steve Paterson, E. Carrol, R. Goodacre

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引用次数: 0

摘要

目的：概念验证研究，强调傅立叶变换红外光谱与 MALDI-TOF MS 相比，结合 WGS 的临床诊断能力。材料与方法：分析了 104 株脑膜炎奈瑟菌、肺炎链球菌、化脓性链球菌和金黄色葡萄球菌的致病性分离物。结果显示傅立叶变换红外光谱的总体预测准确率为 99.6%，MALDI-TOF-MS 为 95.8%。与 MALDI-TOF-MS 相比，傅立叶变换红外光谱对脑膜炎双球菌血清群的分析更为准确。通过傅立叶变换红外光谱和 WGS 分析，化脓性链球菌的系统发育关系相似，但金黄色葡萄球菌和肺炎双球菌的系统发育关系不相似。肺炎双球菌的临床严重程度与锌ABC转运体和DNA修复基因有关，而金黄色葡萄球菌的细胞壁蛋白（生物膜形成、抗生素和补体渗透性）则与WGS有关。结论傅立叶变换红外光谱作为一种有前途的诊断工具，值得临床进一步评估。

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Bacterial discrimination by Fourier transform infrared spectroscopy, MALDI-mass spectrometry and whole-genome sequencing.

Aim: Proof-of-concept study, highlighting the clinical diagnostic ability of FT-IR compared with MALDI-TOF MS, combined with WGS. Materials & methods: 104 pathogenic isolates of Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus pyogenes and Staphylococcus aureus were analyzed. Results: Overall prediction accuracy was 99.6% in FT-IR and 95.8% in MALDI-TOF-MS. Analysis of N. meningitidis serogroups was superior in FT-IR compared with MALDI-TOF-MS. Phylogenetic relationship of S. pyogenes was similar by FT-IR and WGS, but not S. aureus or S. pneumoniae. Clinical severity was associated with the zinc ABC transporter and DNA repair genes in S. pneumoniae and cell wall proteins (biofilm formation, antibiotic and complement permeability) in S. aureus via WGS. Conclusion: FT-IR warrants further clinical evaluation as a promising diagnostic tool.

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.