Kaiqin Chen, R. Tao, Y. Qu, Junnan Lu, Yuan Ping, Yilun Zhang, Pengyu Chen, Chengtao Li
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引用次数: 0
摘要
与常染色体和 Y 染色体遗传标记不同,X 染色体短串联重复序列(X-STR)在特定情况下是不可或缺的。DXS10135、DXS6797、DXS10074、DXS7424、DXS9902、DXS7423、DXS10148、DXS10162、DXS6809、GATA31E08、DXS6803、DXS10075、DXS6807、DXS10164、DXS6789、DXS10146、DXS981、DXS9895、DXS101、DXS6800、DXS907 和 DXS8377)以及 Amelogenin 基因座。在本研究中,我们根据 SWGDAM(DNA 分析方法科学工作组)的指导原则验证了其在法医鉴定中的适用性,包括 PCR 条件、精确度、准确性、案件类型样本、灵敏度、重复性、再现性、物种特异性、稳定性、滞后计算和 DNA 混合物。此外,还利用 577 个汉族个体研究了该系统在法医人口遗传学中的实用性。结果表明,该系统灵敏、稳定、可靠,适用于典型的法医案例。随后的人群调查证实,该系统在法医应用中是一种有效的辅助工具。
Validation and forensic application of a new 36 X-STR loci multiplex system
X-chromosomal short tandem repeats (X-STRs) are indispensable in specific cases, distinct from autosomal and Y chromosome genetic markers. SureID® X37 is an innovative six-color fluorescence multiplex detection system that can simultaneously amplify 36 X-STR loci (including DXS6795, DXS7132, DXS8378, DXS10101, DXS10103, DXS10079, DXS10134, GATA172D05, HPRTB, DXS6810, DXS10135, DXS6797, DXS10074, DXS7424, DXS9902, DXS7423, DXS10148, DXS10162, DXS6809, GATA31E08, DXS6803, DXS10075, DXS6807, DXS10164, DXS6789, DXS10146, DXS981, DXS9895, DXS101, DXS6800 DXS907, and DXS8377) and the Amelogenin locus. In this study, we validated its suitability for forensic identification per SWGDAM (Scientific Working Group on DNA analysis methods) guidelines, including PCR conditions, precision, accuracy, case-type samples, sensitivity, repeatability, reproducibility, species specificity, stability, stutter calculation, and DNA mixtures. Additionally, 577 Han Chinese individuals were used to investigate the utility of the system in forensic population genetics. The results indicated that the system is sensitive, stable, and reliable, and is suitable for typical forensic case. Subsequent population investigations confirmed that it serves as a potent supplementary tool in forensic applications.
期刊介绍:
Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance.
Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.