{"title":"烟草水蒸气素 NtAQP1 和人类水蒸气素 hAQP1 对 Synechococcus 的单细胞光合作用做出了贡献。","authors":"Franziska M. Joseph, Ralf Kaldenhoff","doi":"10.1111/boc.202470003","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background Information</h3>\n \n <p>Aquaporins are H<sub>2</sub>O-permeable membrane protein pores. However, some aquaporins are also permeable to other substances such as CO<sub>2</sub>. In higher plants, overexpression of such aquaporins has already led to an enhanced photosynthetic performance due to improved CO<sub>2</sub> mesophyll conductance. In this work, we investigated the effects of such aquaporins on unicellular photosynthetically active organisms, specifically cyanobacteria.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Overexpression of aquaporins NtAQP1 or hAQP1 that might have a function to improve CO<sub>2</sub> membrane permeability lead to increased photosynthesis rates in the cyanobacterium <i>Synechococcus sp</i>. PCC7002 as concluded by the rate of evolved O<sub>2</sub>. A shift in the Plastoquinone pool state of the cells supports our findings. Water permeable aquaporins without CO<sub>2</sub> permeability, such as NtPIP2;1, do not have this effect.</p>\n </section>\n \n <section>\n \n <h3> Conclusions and Significance</h3>\n \n <p>We conclude that also in single cell organisms like cyanobacteria, membrane CO<sub>2</sub> conductivity could be rate limiting and CO<sub>2</sub>-porins reduce the respective membrane resistance. We could show that besides the tobacco aquaporin NtAQP1 also the human hAQP1 most likely functions as CO<sub>2</sub> diffusion facilitator in the photosynthesis assay.</p>\n </section>\n </div>","PeriodicalId":8859,"journal":{"name":"Biology of the Cell","volume":"116 6","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/boc.202470003","citationCount":"0","resultStr":"{\"title\":\"Tobacco aquaporin NtAQP1 and human aquaporin hAQP1 contribute to single cell photosynthesis in Synechococcus\",\"authors\":\"Franziska M. Joseph, Ralf Kaldenhoff\",\"doi\":\"10.1111/boc.202470003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background Information</h3>\\n \\n <p>Aquaporins are H<sub>2</sub>O-permeable membrane protein pores. However, some aquaporins are also permeable to other substances such as CO<sub>2</sub>. In higher plants, overexpression of such aquaporins has already led to an enhanced photosynthetic performance due to improved CO<sub>2</sub> mesophyll conductance. In this work, we investigated the effects of such aquaporins on unicellular photosynthetically active organisms, specifically cyanobacteria.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Overexpression of aquaporins NtAQP1 or hAQP1 that might have a function to improve CO<sub>2</sub> membrane permeability lead to increased photosynthesis rates in the cyanobacterium <i>Synechococcus sp</i>. PCC7002 as concluded by the rate of evolved O<sub>2</sub>. A shift in the Plastoquinone pool state of the cells supports our findings. Water permeable aquaporins without CO<sub>2</sub> permeability, such as NtPIP2;1, do not have this effect.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions and Significance</h3>\\n \\n <p>We conclude that also in single cell organisms like cyanobacteria, membrane CO<sub>2</sub> conductivity could be rate limiting and CO<sub>2</sub>-porins reduce the respective membrane resistance. 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引用次数: 0
摘要
背景资料水汽素是一种透水蒸气的膜蛋白孔。然而，有些水汽素也能渗透其他物质，如二氧化碳。在高等植物中，过量表达这类水汽素已能改善叶绿体对 CO2 的传导，从而提高光合作用的性能。结果在蓝藻 Synechococcus sp. PCC7002 中，过量表达可能具有改善 CO2 膜通透性功能的水汽素 NtAQP1 或 hAQP1 可提高光合作用速率，这一点可通过 O2 的进化速率得出结论。细胞中塑醌池状态的变化支持了我们的发现。我们的结论是，在蓝藻等单细胞生物中，膜的 CO2 传导性也可能限制速率，而 CO2-孔蛋白可降低相应的膜电阻。我们可以证明，除了烟草水孔蛋白 NtAQP1 外，人类 hAQP1 也很有可能在光合作用试验中起到二氧化碳扩散促进剂的作用。
Tobacco aquaporin NtAQP1 and human aquaporin hAQP1 contribute to single cell photosynthesis in Synechococcus
Background Information
Aquaporins are H2O-permeable membrane protein pores. However, some aquaporins are also permeable to other substances such as CO2. In higher plants, overexpression of such aquaporins has already led to an enhanced photosynthetic performance due to improved CO2 mesophyll conductance. In this work, we investigated the effects of such aquaporins on unicellular photosynthetically active organisms, specifically cyanobacteria.
Results
Overexpression of aquaporins NtAQP1 or hAQP1 that might have a function to improve CO2 membrane permeability lead to increased photosynthesis rates in the cyanobacterium Synechococcus sp. PCC7002 as concluded by the rate of evolved O2. A shift in the Plastoquinone pool state of the cells supports our findings. Water permeable aquaporins without CO2 permeability, such as NtPIP2;1, do not have this effect.
Conclusions and Significance
We conclude that also in single cell organisms like cyanobacteria, membrane CO2 conductivity could be rate limiting and CO2-porins reduce the respective membrane resistance. We could show that besides the tobacco aquaporin NtAQP1 also the human hAQP1 most likely functions as CO2 diffusion facilitator in the photosynthesis assay.
期刊介绍：
The journal publishes original research articles and reviews on all aspects of cellular, molecular and structural biology, developmental biology, cell physiology and evolution. It will publish articles or reviews contributing to the understanding of the elementary biochemical and biophysical principles of live matter organization from the molecular, cellular and tissues scales and organisms.
This includes contributions directed towards understanding biochemical and biophysical mechanisms, structure-function relationships with respect to basic cell and tissue functions, development, development/evolution relationship, morphogenesis, stem cell biology, cell biology of disease, plant cell biology, as well as contributions directed toward understanding integrated processes at the organelles, cell and tissue levels. Contributions using approaches such as high resolution imaging, live imaging, quantitative cell biology and integrated biology; as well as those using innovative genetic and epigenetic technologies, ex-vivo tissue engineering, cellular, tissue and integrated functional analysis, and quantitative biology and modeling to demonstrate original biological principles are encouraged.
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