整合液相色谱-串联质谱代谢组学和高通量测序技术揭示中国不同地区传统腌制芥菜块茎的理化特征和细菌多样性

IF 4.3 3区 材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Siyu Liu, Jiaqi Luo, Xiayu Liu, Ying Shi, Qihe Chen
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引用次数: 0

摘要

本研究旨在调查和比较中国不同地区传统腌制芥菜块茎的理化性质和细菌多样性。 这些腌菜分别来自宁波(NB)、内蒙古(NMG)和哈尔滨(HEB)。pH 值由 pH 计监测。可滴定酸度和盐度通过滴定分析法测定。亚硝酸盐含量采用 N-(1-萘基)-乙二胺二盐酸盐分光光度法测定。有机酸采用液相色谱-串联质谱法(LC-MS/MS)检测。细菌多样性通过 Illumina 高通量测序平台进行检测。 各地腌制芥菜块茎的理化性质存在明显差异,其中北部湾腌菜的 pH 值、盐度和亚硝酸盐含量最高,可滴定酸度最低。重要的是,可滴定酸度较高的腌菜亚硝酸盐和盐分含量相对较低。共检测到 63 种有机酸,其中以乳酸和琥珀酸最多。此外,与 NB 酱腌菜相比,NMG 和 HEB 样品的有机酸组成更为相似。此外,生物信息分析表明,在所有样品中,固着菌和变形菌是主要的细菌门类。其中,乳酸杆菌是主要菌属。HEB 酱腌菜中的细菌数量最多。进一步的相关分析表明,细菌群落与 11 个内部理化参数之间存在很强的相关性。 这项研究有助于更好地了解不同地区腌制芥菜块茎的理化特性和细菌多样性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Integrative liquid chromatography-tandem mass spectrometry metabolomics and high-throughput sequencing technology reveal physicochemical characteristics and bacterial diversity of traditionally pickled mustard tuber from different regions of China
This work aimed to investigate and compare the physicochemical properties and bacterial diversity of traditionally pickled mustard tuber from different regions in China. The pickles were obtained from Ningbo (NB), Nei Monggol (NMG), and Harbin (HEB). The pH was monitored by a pH meter. The titratable acidity and salinity were determined via titrimetric analysis. The nitrite content was measured using N-(1-naphthyl)-ethylenediamine dihydrochloride spectrophotometric method. The organic acids were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The bacterial diversity was conducted through Illumina high-throughput sequencing platform. There were significant differences in the physicochemical properties of pickled mustard tuber from various locations, with the NB pickles having the highest pH, salinity, and nitrite content and the lowest titratable acidity. Importantly, pickles with higher titratable acidity levels had relatively lower nitrite and salinity contents. A total of 63 organic acids were detected, with lactic acid and succinic acid being the most prevalent. Moreover, the organic acid compositions of the NMG and HEB samples were more similar than those of the NB pickles. Additionally, bioinformatic analysis revealed that Firmicutes and Proteobacteria appeared as the predominant bacterial phyla in all samples. Among these, Lactobacillus was the major genera. The most abundant bacteria were found in the HEB pickles. Further correlation analysis showed that there was a strong correlation between bacterial community and 11 internal physicochemical parameters. This study could contribute to a better understanding of the physicochemical characteristics and bacterial diversity of pickled mustard tuber from different locations.
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来源期刊
CiteScore
7.20
自引率
4.30%
发文量
567
期刊介绍: ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric. Indexed/​Abstracted: Web of Science SCIE Scopus CAS INSPEC Portico
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