{"title":"整合液相色谱-串联质谱代谢组学和高通量测序技术揭示中国不同地区传统腌制芥菜块茎的理化特征和细菌多样性","authors":"Siyu Liu, Jiaqi Luo, Xiayu Liu, Ying Shi, Qihe Chen","doi":"10.1093/fqsafe/fyae022","DOIUrl":null,"url":null,"abstract":"\n \n \n This work aimed to investigate and compare the physicochemical properties and bacterial diversity of traditionally pickled mustard tuber from different regions in China.\n \n \n \n The pickles were obtained from Ningbo (NB), Nei Monggol (NMG), and Harbin (HEB). The pH was monitored by a pH meter. The titratable acidity and salinity were determined via titrimetric analysis. The nitrite content was measured using N-(1-naphthyl)-ethylenediamine dihydrochloride spectrophotometric method. The organic acids were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The bacterial diversity was conducted through Illumina high-throughput sequencing platform.\n \n \n \n There were significant differences in the physicochemical properties of pickled mustard tuber from various locations, with the NB pickles having the highest pH, salinity, and nitrite content and the lowest titratable acidity. Importantly, pickles with higher titratable acidity levels had relatively lower nitrite and salinity contents. A total of 63 organic acids were detected, with lactic acid and succinic acid being the most prevalent. Moreover, the organic acid compositions of the NMG and HEB samples were more similar than those of the NB pickles. Additionally, bioinformatic analysis revealed that Firmicutes and Proteobacteria appeared as the predominant bacterial phyla in all samples. Among these, Lactobacillus was the major genera. The most abundant bacteria were found in the HEB pickles. Further correlation analysis showed that there was a strong correlation between bacterial community and 11 internal physicochemical parameters.\n \n \n \n This study could contribute to a better understanding of the physicochemical characteristics and bacterial diversity of pickled mustard tuber from different locations.\n","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":"52 13","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Integrative liquid chromatography-tandem mass spectrometry metabolomics and high-throughput sequencing technology reveal physicochemical characteristics and bacterial diversity of traditionally pickled mustard tuber from different regions of China\",\"authors\":\"Siyu Liu, Jiaqi Luo, Xiayu Liu, Ying Shi, Qihe Chen\",\"doi\":\"10.1093/fqsafe/fyae022\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"\\n \\n \\n This work aimed to investigate and compare the physicochemical properties and bacterial diversity of traditionally pickled mustard tuber from different regions in China.\\n \\n \\n \\n The pickles were obtained from Ningbo (NB), Nei Monggol (NMG), and Harbin (HEB). The pH was monitored by a pH meter. The titratable acidity and salinity were determined via titrimetric analysis. The nitrite content was measured using N-(1-naphthyl)-ethylenediamine dihydrochloride spectrophotometric method. The organic acids were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The bacterial diversity was conducted through Illumina high-throughput sequencing platform.\\n \\n \\n \\n There were significant differences in the physicochemical properties of pickled mustard tuber from various locations, with the NB pickles having the highest pH, salinity, and nitrite content and the lowest titratable acidity. Importantly, pickles with higher titratable acidity levels had relatively lower nitrite and salinity contents. A total of 63 organic acids were detected, with lactic acid and succinic acid being the most prevalent. Moreover, the organic acid compositions of the NMG and HEB samples were more similar than those of the NB pickles. Additionally, bioinformatic analysis revealed that Firmicutes and Proteobacteria appeared as the predominant bacterial phyla in all samples. Among these, Lactobacillus was the major genera. The most abundant bacteria were found in the HEB pickles. Further correlation analysis showed that there was a strong correlation between bacterial community and 11 internal physicochemical parameters.\\n \\n \\n \\n This study could contribute to a better understanding of the physicochemical characteristics and bacterial diversity of pickled mustard tuber from different locations.\\n\",\"PeriodicalId\":3,\"journal\":{\"name\":\"ACS Applied Electronic Materials\",\"volume\":\"52 13\",\"pages\":\"\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2024-04-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Electronic Materials\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1093/fqsafe/fyae022\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/fqsafe/fyae022","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
Integrative liquid chromatography-tandem mass spectrometry metabolomics and high-throughput sequencing technology reveal physicochemical characteristics and bacterial diversity of traditionally pickled mustard tuber from different regions of China
This work aimed to investigate and compare the physicochemical properties and bacterial diversity of traditionally pickled mustard tuber from different regions in China.
The pickles were obtained from Ningbo (NB), Nei Monggol (NMG), and Harbin (HEB). The pH was monitored by a pH meter. The titratable acidity and salinity were determined via titrimetric analysis. The nitrite content was measured using N-(1-naphthyl)-ethylenediamine dihydrochloride spectrophotometric method. The organic acids were detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The bacterial diversity was conducted through Illumina high-throughput sequencing platform.
There were significant differences in the physicochemical properties of pickled mustard tuber from various locations, with the NB pickles having the highest pH, salinity, and nitrite content and the lowest titratable acidity. Importantly, pickles with higher titratable acidity levels had relatively lower nitrite and salinity contents. A total of 63 organic acids were detected, with lactic acid and succinic acid being the most prevalent. Moreover, the organic acid compositions of the NMG and HEB samples were more similar than those of the NB pickles. Additionally, bioinformatic analysis revealed that Firmicutes and Proteobacteria appeared as the predominant bacterial phyla in all samples. Among these, Lactobacillus was the major genera. The most abundant bacteria were found in the HEB pickles. Further correlation analysis showed that there was a strong correlation between bacterial community and 11 internal physicochemical parameters.
This study could contribute to a better understanding of the physicochemical characteristics and bacterial diversity of pickled mustard tuber from different locations.
期刊介绍:
ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric.
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