伊萨洛蝎毒素肽通过嗜水气单胞菌挑战草鱼免疫器官的 JAKs/STATs 信号通路增强其免疫功能

Yuanlin He, Qiyu Hu, Xiaoqiu Zhou, Pei Wu, Weidan Jiang, Yang Liu, Xiaowan Jin, Hongmei Ren, Lin Feng
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引用次数: 0

摘要

为了研究伊沙洛蝎毒素肽(IsCT)对草鱼(Ctenopharyngodon idella)免疫器官(头肾、脾脏和皮肤)免疫功能的影响,给 540 尾鱼(136.88 ± 0.72 克)添加不同剂量的伊沙洛蝎毒素肽(0、0.6、1.2、1.8、2.4 和 3.0 毫克/千克饲料),为期 60 天。然后,从每组中随机挑选 24 条鱼接种嗜水气单胞菌 6 天。我们的研究结果表明,适当的 IsCT 补充:(1)减轻了皮肤发病率和头肾脾的组织病理学结构变化(p < 0.05),确保了免疫器官结构的完整性;(2)提高了免疫物质的活性和表达(p < 0.05),进而增加了免疫器官的功能,促进了免疫反应的发生;(3)通过调节Janus激酶/信号转导子和转录激活子(JAKs/STATs)信号通路,使抗炎细胞因子的mRNA表达增加,促炎细胞因子的mRNA表达减少,进而增加了免疫器官的功能,减轻了炎症反应(P<0.05)。然而,添加 IsCT 并不影响免疫器官中 IL-12p35、STAT2 和 STAT3a 的表达。最终,本研究提供了证据,证明在草鱼受到嗜水气单胞菌挑战后,IsCT可通过JAKs/STATs信号通路增强免疫器官的免疫功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Isalo scorpion cytotoxic peptide-strengthened immune function through the JAKs/STATs signaling pathway of the immune organ of grass carp (Ctenopharyngodon idella) challenged with Aeromonas hydrophila

Isalo scorpion cytotoxic peptide-strengthened immune function through the JAKs/STATs signaling pathway of the immune organ of grass carp (Ctenopharyngodon idella) challenged with Aeromonas hydrophila

To investigate the impact of Isalo scorpion cytotoxic peptide (IsCT) on the immune function of immune organ (head kidney, spleen, and skin) of grass carp (Ctenopharyngodon idella), 540 fish (136.88 ± 0.72 g) were supplied with a different amount of IsCT (0, 0.6, 1.2, 1.8, 2.4, and 3.0 mg/kg diet) through a period of 60 days. Afterward, 24 fish were randomly selected from each group and were inoculated with Aeromonas hydrophila for a period of 6 days. Our findings suggested that appropriate IsCT complementation: (1) attenuated skin morbidity and histopathological structural changes in the head kidney and spleen (p < 0.05), which ensured the structural integrity of the immune organs; (2) increased the activity and expression of immune substances (p < 0.05), which in turn increased the function of the immune organs, promoting immune responses; (3) through the regulation of the Janus kinase/signal transducers and activators of transcription (JAKs/STATs) signaling pathway, the mRNA expression of anti-inflammatory cytokines increased and the mRNA expression of pro-inflammatory cytokines decreased, which in turn increased the function of the immune organs, reducing the inflammatory response (p < 0.05). However, the addition of IsCT did not affect the expression of IL-12p35, STAT2, and STAT3a in the immune organ. Ultimately, this study provided evidence that IsCT enhanced immune function via the JAKs/STATs signaling pathway in the immune organ in grass carp after challenged with Aeromonas hydrophila.

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