揭示气液界面铜绿假单胞菌生物膜的分子动力学。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-04-25 DOI:10.2217/fmb-2023-0234

K. Sung, Miseon Park, Jung-Hwa Chon, Ohgew Kweon, Saeed Khan

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引用次数: 0

摘要

目的：本研究旨在通过全局蛋白质组分析探究铜绿假单胞菌 PA14 气液界面（ALI）生物膜随时间变化的动态。材料与方法：收集铜绿假单胞菌 PA14 ALI 生物膜样本 48-144 小时，进行差异表达分析，以确定每个时间点的不同蛋白质。结果确定了一组一致的 778 种蛋白质，其表达随时间变化。上调的蛋白质主要与 "氨基酸转运和代谢 "有关。与生物膜相关的途径，包括 cAMP/Vfr 和 QS，都发生了显著变化。鞭毛比纤毛虫更有影响力，尤其是在生物膜发展的早期。与毒力、转运体和铁有关的蛋白质在整个过程中都有不同的表达。结论这些发现加深了我们对 ALI 生物膜发展的了解。

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Unraveling the molecular dynamics of Pseudomonas aeruginosa biofilms at the air-liquid interface.

Aim: The aim of this study was to probe the dynamics of Pseudomonas aeruginosa PA14 air-liquid interface (ALI) biofilms over time through global proteomic analysis. Materials & methods: P. aeruginosa PA14 ALI biofilm samples, collected over 48-144 h, underwent differential expression analysis to identify varying proteins at each time point. Results: A consistent set of 778 proteins was identified, with variable expression over time. Upregulated proteins were mainly linked to 'amino acid transport and metabolism'. Biofilm-related pathways, including cAMP/Vfr and QS, underwent significant changes. Flagella were more influential than pili, especially in early biofilm development. Proteins associated with virulence, transporters and iron showed differential expression throughout. Conclusion: The findings enhance our understanding of ALI biofilm development.

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464

期刊介绍： ACS Applied Bio Materials is an interdisciplinary journal publishing original research covering all aspects of biomaterials and biointerfaces including and beyond the traditional biosensing, biomedical and therapeutic applications. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrates knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important bio applications. The journal is specifically interested in work that addresses the relationship between structure and function and assesses the stability and degradation of materials under relevant environmental and biological conditions.