揭示气液界面铜绿假单胞菌生物膜的分子动力学。

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2024-04-25 DOI:10.2217/fmb-2023-0234

K. Sung, Miseon Park, Jung-Hwa Chon, Ohgew Kweon, Saeed Khan

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引用次数: 0

摘要

目的：本研究旨在通过全局蛋白质组分析探究铜绿假单胞菌 PA14 气液界面（ALI）生物膜随时间变化的动态。材料与方法：收集铜绿假单胞菌 PA14 ALI 生物膜样本 48-144 小时，进行差异表达分析，以确定每个时间点的不同蛋白质。结果确定了一组一致的 778 种蛋白质，其表达随时间变化。上调的蛋白质主要与 "氨基酸转运和代谢 "有关。与生物膜相关的途径，包括 cAMP/Vfr 和 QS，都发生了显著变化。鞭毛比纤毛虫更有影响力，尤其是在生物膜发展的早期。与毒力、转运体和铁有关的蛋白质在整个过程中都有不同的表达。结论这些发现加深了我们对 ALI 生物膜发展的了解。

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Unraveling the molecular dynamics of Pseudomonas aeruginosa biofilms at the air-liquid interface.

Aim: The aim of this study was to probe the dynamics of Pseudomonas aeruginosa PA14 air-liquid interface (ALI) biofilms over time through global proteomic analysis. Materials & methods: P. aeruginosa PA14 ALI biofilm samples, collected over 48-144 h, underwent differential expression analysis to identify varying proteins at each time point. Results: A consistent set of 778 proteins was identified, with variable expression over time. Upregulated proteins were mainly linked to 'amino acid transport and metabolism'. Biofilm-related pathways, including cAMP/Vfr and QS, underwent significant changes. Flagella were more influential than pili, especially in early biofilm development. Proteins associated with virulence, transporters and iron showed differential expression throughout. Conclusion: The findings enhance our understanding of ALI biofilm development.

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.