大肠杆菌中 yghZ 的缺失可促进以木糖为碳源的糠醛的生长。

IF 2.2 4区 生物学 Q3 MICROBIOLOGY
Bilal Jilani
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引用次数: 0

摘要

木质纤维素生物质需要进行热酸预处理,以使其适于微生物代谢,并在戊糖分解过程中产生糠醛。糠醛对微生物的新陈代谢具有毒性,会降低微生物的生产率,增加生产成本。yghZ 基因编码一种 NADPH 依赖性醛还原酶,本研究询问删除该基因是否会提高大肠杆菌宿主对糠醛的耐受性。在 AM1 最小培养基中,以 5% 木糖为碳源,测试了 ∆yghZ 菌株-SSK201 对糠醛的耐受性。与亲本菌株 SSK42 相比,在 1.0 g/L 糠醛存在下培养 96 小时后,携带菌株 SSK201 的生物量增加了 4.5 倍,糠醛浓度降低了 2 倍,特定生长率(µ)增加了 15.75 倍。当在 AM1 培养基中将碳源转换为葡萄糖时,SSK201 的糠醛耐受性优势得以保留,而在富含 LB 的培养基中则丧失了这一优势。这些发现有可能推广到生物反应器培养下的水解物培养基中,并观察 ∆yghZ 宿主的生长优势。它具有产生强健工业菌株的潜力,能以经济高效的方式将木质纤维素碳转化为感兴趣的代谢物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Deletion of yghZ in Escherichia coli promotes growth in presence of furfural with xylose as carbon source.
Thermo-acidic pretreatment of lignocellulosic biomass is required to make it amenable to microbial metabolism and results in generation of furfural due to breakdown of pentose sugars. Furfural is toxic to microbial metabolism and results in reduced microbial productivity and increased production costs. This study asks if deletion of yghZ gene which encodes a NADPH-dependent aldehyde reductase enzyme results in improved furfural tolerance in Escherichia coli host. The ∆yghZ strain-SSK201-was tested for tolerance to furfural in presence of 5% xylose as a carbon source in AM1 minimal medium. At 96 h and in presence of 1.0 g/L furfural, the culture harboring strain SSK201 displayed 4.5-fold higher biomass, 2-fold lower furfural concentration and 15.75-fold higher specific growth rate (µ) as compared to the parent strain SSK42. The furfural tolerance advantage of SSK201 was retained when the carbon source was switched to glucose in AM1 medium and was lost in rich LB medium. The findings have potential to be scaled up to a hydrolysate culture medium, which contains furan inhibitors and lack nutritionally rich components, under bioreactor cultivation and observe growth advantage of the ∆yghZ host. It harbors potential to generate robust industrial strains which can convert lignocellulosic carbon into metabolites of interest in a cost-efficient manner.
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来源期刊
Fems Microbiology Letters
Fems Microbiology Letters 生物-微生物学
CiteScore
4.30
自引率
0.00%
发文量
112
审稿时长
1.9 months
期刊介绍: FEMS Microbiology Letters gives priority to concise papers that merit rapid publication by virtue of their originality, general interest and contribution to new developments in microbiology. All aspects of microbiology, including virology, are covered. 2019 Impact Factor: 1.987, Journal Citation Reports (Source Clarivate, 2020) Ranking: 98/135 (Microbiology) The journal is divided into eight Sections: Physiology and Biochemistry (including genetics, molecular biology and ‘omic’ studies) Food Microbiology (from food production and biotechnology to spoilage and food borne pathogens) Biotechnology and Synthetic Biology Pathogens and Pathogenicity (including medical, veterinary, plant and insect pathogens – particularly those relating to food security – with the exception of viruses) Environmental Microbiology (including ecophysiology, ecogenomics and meta-omic studies) Virology (viruses infecting any organism, including Bacteria and Archaea) Taxonomy and Systematics (for publication of novel taxa, taxonomic reclassifications and reviews of a taxonomic nature) Professional Development (including education, training, CPD, research assessment frameworks, research and publication metrics, best-practice, careers and history of microbiology) If you are unsure which Section is most appropriate for your manuscript, for example in the case of transdisciplinary studies, we recommend that you contact the Editor-In-Chief by email prior to submission. Our scope includes any type of microorganism - all members of the Bacteria and the Archaea and microbial members of the Eukarya (yeasts, filamentous fungi, microbial algae, protozoa, oomycetes, myxomycetes, etc.) as well as all viruses.
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