Ajuga chamaepitys (L.) Schreb.提取物的植物化学、体外、体内和硅学研究

Plants Pub Date : 2024-04-25 DOI:10.3390/plants13091192
Elis Ionus, Verginica Schröder, C. Chițescu, L. Bucur, C. Lupu, D. Dumitrescu, Liliana Popescu, D. Mihai, O. T. Olaru, G. Nițulescu, R. Boscencu, C. Gîrd
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引用次数: 0

摘要

本研究的重点是从 Ajuga chamaepitys (L.) Schreb 树种中提取的干提取物的化学特征,评估其抗氧化特性、毒性和硅学特征。干提取物的定量分析显示,其中含有大量植物化学化合物:59.932 ± 21.167 毫克芦丁当量(mg REs)/克干重,45.864 ± 4.434 毫克绿原酸当量(mg ChAEs)/克干重,83.307 ± 3.989 毫克单宁酸当量(TAEs)/克干重。通过 UHPLC-HRMS/MS 方法,定量分析了以下主要化合物:咖啡酸(3253.8 μg/g 提取物)和山柰醇(3041.5 μg/g 提取物);定量分析了超过 11 种多酚化合物(染料木苷 730.2 μg/g 提取物、柚皮苷 395 μg/g 提取物、芹菜苷 325.7 μg/g 提取物、高良姜素 283.3 μg/g 提取物、阿魏酸 254.3 μg/g 提取物、对香豆素 198.2 μg/g 提取物、芦丁 110.6 μg/g 提取物、菊黄素 90.22 μg/g 提取物、丁香酸 84.2 μg/g 提取物、松果菊素 32.7 μg/g 提取物、鞣花酸 18.2 μg/g 提取物)。抗氧化活性与植物化学物质的含量成正比:IC50DPPH = 483.6 ± 41.4 µg/mL, IC50ABTS-+ = 127.4 ± 20.2 µg/mL, EC50FRAP = 491.6 ± 2 µg/mL.研究发现,萃取物对黄颡鱼幼虫的细胞毒性较低。硅学研究强调了芹菜素、高良姜素和山奈酚抑制蛋白激酶 CDK5 和 GSK-3b 活性的可能性,这可能有助于治疗神经退行性病变和神经性疼痛。有必要开展进一步的研究,以确认预测的分子作用机制,并进一步研究在神经系统疾病动物模型中的治疗潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phytochemical, In Vitro, In Vivo, and In Silico Research on the Extract of Ajuga chamaepitys (L.) Schreb.
The present study focuses on the chemical characterization of a dry extract obtained from the species Ajuga chamaepitys (L.) Schreb, evaluating its antioxidant properties, toxicity, and in silico profile. Quantitative analysis of the dry extract revealed a notable amount of phytochemical compounds: 59.932 ± 21.167 mg rutin equivalents (mg REs)/g dry weight, 45.864 ± 4.434 mg chlorogenic acid equivalents (mg ChAEs)/g dry weight and, respectively, 83.307 ± 3.989 mg tannic acid equivalents (TAEs)/g dry weight. By UHPLC-HRMS/MS, the following were quantified as major compounds: caffeic acid (3253.8 μg/g extract) and kaempherol (3041.5 μg/g extract); more than 11 types of polyphenolic compounds were quantified (genistin 730.2 μg/g extract, naringenin 395 μg/g extract, apigenin 325.7 μg/g extract, galangin 283.3 μg/g extract, ferulic acid 254.3 μg/g extract, p-coumaric acid 198.2 μg/g extract, rutin 110.6 μg/g extract, chrysin 90.22 μg/g extract, syringic acid 84.2 μg/g extract, pinocembrin 32.7 μg/g extract, ellagic acid 18.2 μg/g extract). The antioxidant activity was in accordance with the amount of phytochemical compounds: IC50DPPH = 483.6 ± 41.4 µg/mL, IC50ABTS•+ = 127.4 ± 20.2 µg/mL, and EC50FRAP = 491.6 ± 2 µg/mL. On the larvae of Artemia sp., it was found that the extract has a low cytotoxic action. In silico studies have highlighted the possibility of inhibiting the activity of protein kinases CDK5 and GSK-3b for apigenin, galangin, and kaempferol, with possible utility for treating neurodegenerative pathologies and neuropathic pain. Further studies are warranted to confirm the predicted molecular mechanisms of action and to further investigate the therapeutic potential in animal models of neurological disorders.
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