Oerskovia flava sp.

IF 1.8 3区 生物学 Q4 MICROBIOLOGY
Wei Wei, Wenjun Guo, Biyue Yang, Kaili Wang, Xinyu Hu, Guanya Ji, Xiangru Xu, Shenkui Liu, Lin Zhu
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引用次数: 0

摘要

JB1-3-2 T降解脱氧雪腐镰刀菌(DON)是从中国东部镇江的一个温室中采集的黄瓜根瘤土壤样本中分离出来的。JB1-3-2 T 菌株是一种革兰氏染色阳性、无运动性的圆形放线菌。该菌株在 15 至 40 ℃(最适温度为 35 ℃)、15%(w/v)NaCl(最适浓度为 3%)、pH 值为 3 和 11(最适浓度为 7)的条件下生长。鉴定出的主要细胞脂肪酸为前异-C15:0、异-C16:0 和前异-C17:0。基因组测序显示基因组大小为 4.11 Mb,DNA G + C 含量为 72.5 mol%。基于 16S rRNA 基因序列的系统发育分析表明,JB1-3-2 T 菌株与 Oerskovia 菌种的模式菌株关系最为密切,与 Oerskovia turbata NRRL B-8019 T 的序列相似度最高(98.2%),与该属其他有效模式菌株的序列相似度为 98.1%。数字 DNA-DNA 杂交(dDDH)和平均核苷酸同一性(ANI)显示,JB1-3-2 T 与 Oerskovia 属的模式菌株之间的亲缘关系分别为 21.8%-22.2%和 77.2%-77.3%。根据基因型、系统发生学、化学分类学、生理学和生物化学特征,提出 Oerskovia flava 为 Oerskovia 属的一个新种,模式菌株为 JB1-3-2 T(= CGMCC 1.18555 T = JCM 35248 T)。此外,该新菌株具有其他 Oerskovia 属物种所不具备的 DON 降解能力,谷胱甘肽-S-转移酶被推测为 JB1-3-2 T 菌株降解 DON 的关键酶。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Oerskovia flava sp. nov., a deoxynivalenol (DON)-degrading actinomycete isolated from the rhizosphere soil of long-term continuous cropping cucumber

Oerskovia flava sp. nov., a deoxynivalenol (DON)-degrading actinomycete isolated from the rhizosphere soil of long-term continuous cropping cucumber

Oerskovia flava sp. nov., a deoxynivalenol (DON)-degrading actinomycete isolated from the rhizosphere soil of long-term continuous cropping cucumber

The deoxynivalenol (DON)-degrading bacterium JB1-3-2 T was isolated from a rhizosphere soil sample of cucumber collected from a greenhouse located in Zhenjiang, Eastern China. The JB1-3-2 T strain is a Gram-stain-positive, nonmotile and round actinomycete. Growth was observed at temperatures between 15 and 40 ℃ (optimum, 35 ℃), in the presence of 15% (w/v) NaCl (optimum, 3%), and at pH 3 and 11 (optimum, 7). The major cellular fatty acids identified were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. Genome sequencing revealed a genome size of 4.11 Mb and a DNA G + C content of 72.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the JB1-3-2 T strain was most closely related to type strains of the Oerskovia species, with the highest sequence similarity to Oerskovia turbata NRRL B-8019 T (98.2%), and shared 98.1% sequence identity with other valid type strains of this genus. Digital DNA‒DNA hybridization (dDDH) and average nucleotide identity (ANI) showed 21.8–22.2% and 77.2–77.3% relatedness, respectively, between JB1-3-2 T and type strains of the genus Oerskovia. Based on genotypic, phylogenetic, chemotaxonomic, physiological and biochemical characterization, Oerskovia flava, a novel species in the genus Oerskovia, was proposed, and the type strain was JB1-3-2 T (= CGMCC 1.18555 T = JCM 35248 T). Additionally, this novel strain has a DON degradation ability that other species in the genus Oerskovia do not possess, and glutathione-S-transferase was speculated to be the key enzyme for strain JB1-3-2 T to degrade DON.

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来源期刊
CiteScore
5.60
自引率
11.50%
发文量
104
审稿时长
3 months
期刊介绍: Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.
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