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利用低温电子断层扫描技术进行膜生物学原位研究

IF 6 2区 生物学 Q1 CELL BIOLOGY
Current Opinion in Cell Biology Pub Date : 2024-04-26 DOI:10.1016/j.ceb.2024.102363
Jenny Keller , Rubén Fernández-Busnadiego
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引用次数: 0

摘要

低温电子断层扫描（cryo-ET）可在近原生环境中对生物样本进行高分辨率三维成像。因此，低温电子断层扫描已成为分析细胞膜未受干扰组织的首选方法。在此，我们将简要讨论当前的低温电子显微镜工作流程及其在原位、基础和病理条件下研究膜生物学的应用。
本文章由计算机程序翻译，如有差异，请以英文原文为准。

In situ studies of membrane biology by cryo-electron tomography

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In situ studies of membrane biology by cryo-electron tomography

Cryo-electron tomography (cryo-ET) allows high resolution 3D imaging of biological samples in near-native environments. Thus, cryo-ET has become the method of choice to analyze the unperturbed organization of cellular membranes. Here, we briefly discuss current cryo-ET workflows and their application to study membrane biology in situ, under basal and pathological conditions.

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来源期刊
Current Opinion in Cell Biology
Current Opinion in Cell Biology 生物-细胞生物学
CiteScore
14.60
自引率
1.30%
发文量
79
审稿时长
93 days
期刊介绍： Current Opinion in Cell Biology (COCEBI) is a highly respected journal that specializes in publishing authoritative, comprehensive, and systematic reviews in the field of cell biology. The journal's primary aim is to provide a clear and readable synthesis of the latest advances in cell biology, helping specialists stay current with the rapidly evolving field. Expert authors contribute to the journal by annotating and highlighting the most significant papers from the extensive body of research published annually, offering valuable insights and saving time for readers by distilling key findings. COCEBI is part of the Current Opinion and Research (CO+RE) suite of journals, which leverages the legacy of editorial excellence, high impact, and global reach to ensure that the journal is a widely read resource integral to scientists' workflow. It is published by Elsevier, a publisher known for its commitment to excellence in scientific publishing and the communication of reproducible biomedical research aimed at improving human health. The journal's content is designed to be an invaluable resource for a diverse audience, including researchers, lecturers, teachers, professionals, policymakers, and students.
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